Detection of
            <i>Schistosoma mansoni</i>
            Membrane Antigens by Immunoblot Analysis of Sera of Patients from Low-Transmission Areas

Cesari, Italo M.; Ballén, Diana; Mendoza, Leydi; Matos, César

doi:10.1128/cdli.12.2.280-286.2005

Cited by 19 publications

(28 citation statements)

References 23 publications

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“…Immunodiagnostic detection of schistosome antibodies is more sensitive and less time consuming than parasitological diagnosis and has thus become a more attractive option for the diagnosis of schistosomiasis (Zhu, 2005). These alternative diagnostic techniques have proven particularly useful for diagnosing atypical forms of schistosomiasis (e.g., infections with low parasite burdens) and in disease surveillance (Doenhoff et al, 2004;Cesari et al, 2005;Sorgho et al, 2005;Sulahian et al, 2005;Enk et al, 2008). In Saudi Arabia, the prevalence of schistosomiasis was 2.2, 2.9, and 2.78/100,000 in 2000, 2004, and 2008 respectively, according to the Saudi Arabia Ministry of Health Statistic Book, (http://www.…”

Section: Introductionmentioning

confidence: 99%

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Elshahawy¹,

Mohamed

Adail

2016

Helminthologia

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SummarySchistosomiasis is a major cause of morbidity and mortality and has been estimated to infect over 200 million people. Diagnosis of schistosomiasis by detection of specifi c antibodies is likely to be more sensitive than the traditional method of diagnosis by detection of eggs in stool or urine. Therefore the present study was the fi rst attempt to highlight the seroepidemiology of schistosomiasis among the general population of Najran City, southern of Saudi Arabia, as well as to achieve the performance of the diagnostic tests used. A total of 180 participants attending King Khaled hospital in Najran Province, Saudi Arabia, over a one year period, from September 2013 to September 2014 were screened for the presence of Schistosoma antibody in their blood serum using an indirect hemagglutination assay (IHA). Specifi c immunoglobulin (Ig) G antibody was evaluated using an enzyme-linked immunosorbent assay (ELISA). Out of the 180 samples of sera tested using IHA, 32 (20 %) were found to be positive with a titer ranging from 1:160 to 1:1280, while 42(23.2 %) revealed Schistosoma IgG. A positive relationship was found between the seroprevalence of schistosomiasis and age of tested participants, especially in the age group of 20-40 years old. Additionally, prevalence of infection was more in males (36 %) than females (7.5 %), and showed statistical significance (P < 0.001). Similarly, there was signifi cant association between the presence of Schistosoma antibodies and the nationality of residence, and education of participants (P < 0.05). The current investigation reveals an alarmingly high prevalence of schistosomiasis among participants in Najran, southern region of Saudi Arabia and this supports an urgent need to re-evaluate the current control measures and implement an integrated, targeted and effective schistosomiasis control measures.

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Section: Introductionmentioning

confidence: 99%

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Elshahawy¹,

Mohamed

Adail

2016

Helminthologia

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“…1a); the antigens migrated similarly and fell into the same range of molecular masses as those characteristically seen by Venezuelan S. mansoni-infected patient sera (SmV) in the same BE preparation (Fig. 1a, (+) control, and Cesari et al 2005). Similar to SmV sera (Cesari et al 2005, Fig. 1a, (+) control), most SmS and the SmE sera reacted predominantly with the 24-28-kDa complex (14/26 and 18/26, respectively) and the 20-kDa (19/26) antigen.…”

Section: Resultsmentioning

confidence: 77%

“…Many membrane-bound schistosome components have been reported to be targets for the host immune response (Sher et al 1974;Smithers et al 1989Smithers et al , 1990Cesari et al 2005;Kusel et al 2007). Some of these components have been described to perform a variety of important physiological functions including transmembrane ion channels, enzymatic activities, and receptors (Tanford and Reynolds 1976;Cesari et al 1981;Low and Zilversmit 1980;Skelly 2005).…”

Section: Discussionmentioning

confidence: 98%

“…The homogenate was ultracentrifuged at 100,000×g/2 h/5°C. The membranous pellet was washed and resuspended in the original volume with Tris-Mg buffer, and the suspension mixed vol/vol with water-saturated n-butanol (Pujol and Cesari 1990;Cesari et al 2005). The resulting emulsion was centrifuged at 14,000×g/15 min/4°C, and the aqueous phase recovered and dialyzed against the Tris-Mg buffer.…”

Section: S Mansoni Antigensmentioning

confidence: 99%

“…Membrane-bound antigens from adult S. mansoni worms extracted with n-butanol (BE antigens) have been proven highly specific for the immunodiagnosis of schistosomiasis mansoni (Pujol et al 1989;Alarcón de Noya et al 1997;Cesari et al 1998Cesari et al , 2002Cesari et al , 2005. Indeed, Venezuelan S. mansoni-infected patient sera detect in BE immunoblots from S. mansoni a series of antigens in the range of ∼8-80 kDa.…”

Section: Introductionmentioning

confidence: 97%

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Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera

et al. 2010

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Antigens present in aqueous n-butanolic extracts (BE) of Schistosoma mansoni (Venezuelan JL strain), Schistosoma intercalatum (Cameroon EDEA strain), and Schistosoma haematobium (Yemen strain) adult worm membranes were compared in immunoblot against sera of patients infected with S. mansoni, S. intercalatum, S. haematobium, Schistosoma japonicum, or Schistosoma mekongi looking for similarities (common antigens) and differences (species-specific antigens). About 17 S. mansoni BE polypeptides (M (r) approximately 8 to >80 kDa) were commonly recognized by S. mansoni-infected patient sera from Venezuela, Senegal, and Ethiopia. S. intercalatum-, S. haematobium-, or S. japonicum-infected sera were almost unreactive with S. mansoni BE. Nonetheless, S. mekongi-infected sera weakly cross-reacted with a approximately 10-15-kDa subset of S. mansoni BE. About 72.7% of S. intercalatum-infected patient sera reacted with a approximately 19-21-kDa complex in S. intercalatum BE and cross-reacted with a similar complex in S. haematobium BE. Conversely, all S. haematobium-infected patient sera reacted with a approximately 19-21-kDa complex in S. haematobium BE and cross-reacted with the approximately 19-21-kDa complex in S. intercalatum BE; S. mansoni- and S. japonicum-infected patient sera did not react with S. intercalatum or S. haematobium BE. Results showed the presence of a common membrane antigen between African schistosome species and species-specific antigens in S. mansoni BE that could be useful to discriminate between species and/or to detect Schistosoma infections.

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Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA)

et al. 2014

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To know if alkaline phosphatase (AP) from schistosomes other than Schistosoma mansoni can be used as diagnostic marker for schistosomiasis in alkaline phosphatase immunocapture assay (APIA), we comparatively tested n-butanol extracts of adult worm membranes from a Venezuelan (JL) strain of S. mansoni (Ven/AWBE/Sm); a Cameroonian (EDEN) strain of Schistosoma intercalatum (Cam/AWBE/Si) and a Yemeni strain of Schistosoma haematobium (Yem/AWBE/Sh). APIA was evaluated with sera of patients from Venezuela, Senegal, and Gabon infected with S. mansoni, from Gabon infected with S. intercalatum or S. haematobium, from Chine infected with Schistosoma japonicum and from Cambodian patients infected with Schistosoma mekongi. Results indicate that 92.5% (37/40) of Venezuela sera, 75% (15/20) of Senegal sera, 39.5% (17/43) of S. haematobium sera, and 19.2% (5/26) S. intercalatum sera were APIA-positive with the Ven/AWBE/Sm preparation. APIA with the Cam/AWBE/Si preparation showed that 53.8% of S. intercalatum-positive sera had anti-AP antibodies, and 51.2% S. haematobium-positive sera cross-immunocapturing the S. intercalatum AP. APIA performed with Yem/AWBE/Sh showed that 55.8% S. haematobium sera were positive. Only two out of nine S. japonicum sera were APIA-positive with the Ven/AWBE/Sm and Cam/AWBE/Si, and no reaction was observed with Cambodian S. mekongi-positive sera. AP activity was shown to be present in all the schistosome species/strains studied. The use of APIA as a tool to explore the APs antigenicity and the presence of Schistosoma sp. infections through the detection of anti-Schistosoma sp. AP antibodies in a host, allowed us to demonstrate the antigenicity of APs of S. mansoni, S. intercalatum, and S. haematobium.

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Detection of Schistosoma mansoni Membrane Antigens by Immunoblot Analysis of Sera of Patients from Low-Transmission Areas

Cited by 19 publications

References 23 publications

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera

Comparative evaluation of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium alkaline phosphatase antigenicity by the alkaline phosphatase immunoassay (APIA)

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