Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera

Cesari, Italo M.; Ballén, Diana; Mendoza, Leydi; Ferrer, Alain; Pointier, Jean‐Pierre; Kombila, Maryvonne; Richard-Lenoble, D; Thèron, André

doi:10.1007/s00436-010-1798-x

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…S. mansoni adult worm and soluble egg antigens were also recently used successfully in the diagnosis of S. mekongi infection (143). Adult Schistosoma worm membrane extracts have been applied in immunoblots for detecting antibodies against S. intercalatum, and an alkaline phosphatase immune capture assay (APIA) has also been developed for diagnosing this infection in patients (144,145). An ELISA detecting specific IgG against schistosomula tegument antigens (ELISA-SmTeg) has shown an improved performance compared to that for an assay detecting antibody against AWA, particularly in diagnosing early-stage acute S. mansoni infections among individuals in a travel group from an area in Brazil where the disease is not endemic, who were infected in a new focus of S. mansoni (146).…”

Section: Antibody Detectionmentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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Section: Antibody Detectionmentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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“…Scale bars 50 μm A. lumbricoides, and T. vitulorum. Similarly, Cesari et al (2010) have shown the presence of a common membrane antigen between African schistosome species and speciesspecific antigens in Schistosoma mansoni BE that could be useful to discriminate between species and/or to detect Schistosoma infections. Moreover, immunolocalization of two novel genes, lactate dehydrogenase A and B, on the tegument of Taenia solium was determined recently by Du et al (2011).…”

Section: Resultsmentioning

confidence: 96%

Immunolocalization of arginine kinase (AK) in Toxocara canis, Toxocara vitulorum, and Ascaris lumbricoides

et al. 2012

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Arginine kinase (AK) is a member of the phosphagen kinase family. AK plays a major role in cellular energy metabolism in invertebrates including nematodes. In the present study, we performed the direct immunofluorescence test to determine the immunolocalization of AK in different stages of the life cycle (eggs, larvae, and adult worms) of Toxocara canis, Toxocara vitulorum, and Ascaris lumbricoides. Our results indicated variable levels of expression of AK in different stages. Moreover, strong fluorescence was observed in cleaving eggs than in dormant eggs. The highest activity of the enzyme was observed in the fully developed eggs. This may be due to high expression of AK in embryonic development, which is associated with increased energy demand due to cleavage and cellular differentiation. Surprisingly, expression of AK is significantly higher in the middle part and posterior end compared to anterior end of the larvae. In addition, AK is highly concentrated in cellular and metabolically active parts of the body such as hypodermis, muscle, intestine, ovaries, oviducts, and uterus, while it is absent in noncellular areas like cuticle. The present study revealed the presence of AK in T. canis, A. lumbricoides, and T. vitulorum and that it plays a major role in energy metabolism of these nematodes. Interestingly, antiserum was prepared against the recombinant T. canis AK and reacts with the native AKs of T. canis, A. lumbricoides, and T. vitulorum. AK levels could vary in relation to maximum potential rates of ATP turnover, oxidative capacity, and energy output. Further studies on subcellular localization of AK in these important helminths provide new information for researchers to develop effective anthelmintics against the parasites of veterinary and of public health importance.

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“…Some of these perform a variety of important physiological functions including transmembrane ion channels, enzymatic activities, and receptors for several molecules (Cesari et al 2010). …”

Section: Discussionmentioning

confidence: 99%

“…However, the possibility of LDL internalization in S. mansoni through LDL receptors in a way similar or not to human receptors cannot be excluded, as seen by Dinguirard and Yoshino (2006) who found LDL internalization through endocytosis in the larval stage of S. mansoni. Therefore, the characterization of tegument proteins is relevant to the improvement of the functional understanding of this structure and the identification of molecules that may act as targets for protective immune responses or may be useful in diagnosis (Abath et al 2000;Cesari et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy of the human low-density lipoprotein interaction with the tegument of Schistosoma mansoni

et al. 2011

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The interaction between host molecules and Schistosoma mansoni has been regarded as a key feature for parasite survival. In this work, scanning electron microscopy was used to study the interaction of human low-density lipoprotein (LDL) with the tegument of the adult worm of S. mansoni. Worms were incubated in RPMI 1640 containing 10% of LPDS and 40 μg LDL/mL during 30, 60, and 120 min. Control worms were processed in the same way, without LDL. After the incubations, the samples were fixed and processed to scanning electron microscopy. The results demonstrated interaction of the LDL particles with the male parasite tegument. Male and female worms incubated without LDL from 0 (control) to 120 min did not show alterations in the tegument. It was observed a larger number of LDL particles on the dorsal region of male adult worm than others regions (anterior, posterior and gynecophoral canal). The female tegument did not show adherence of LDL. Aggregates on the tegument of the male worm were in greater number and size in the incubation times of 30 and 60 min than 120 min. The comparison between 30 and 120 min of incubation showed that the particles' size diminished from 2,650-860 nm to 634-363 nm, respectively. Such reduction can be due to the capture and the use of the lipids by the worm. Therefore, the internalization of lipids from LDL by the male worms seems to be a mechanism independent of endocytosis. Differences between males and females suggest lipid transference from male to female through gynecophoral canal.

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Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera

Cited by 10 publications

References 31 publications

Advances in the Diagnosis of Human Schistosomiasis

Advances in the Diagnosis of Human Schistosomiasis

Immunolocalization of arginine kinase (AK) in Toxocara canis, Toxocara vitulorum, and Ascaris lumbricoides

Scanning electron microscopy of the human low-density lipoprotein interaction with the tegument of Schistosoma mansoni

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