Detection of<i>mec</i>a gene from methicillin resistant<i>staphylococcus aureus</i>isolates of north sumatera

Nasution, Gabriella Septiani; Suryanto, Dwi; Kusumawati, R. Lia

doi:10.1088/1755-1315/130/1/012026

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…53.85% isolates of MRSA were reported sensitive to vancomycin during present study. Existance of vancomycin resistant MRSA during present study was well supported by Shah et al (2019) [18] , Nasution et al (2018) [17] and Al-Zaidi and Al-Sulami (2013) [15] . In contrast, Mahmood et al (2010) [23] , Selvabai et al (2017) [20] , Sonth et al (2015) [26] , Li et al (2017) [27] , Sharma et al (2017) [28] and Krishnan et al (2019) [21] reported 100% sensitivity of MRSA to vancomycin.…”

Section: Antibiogram Of Mrsasupporting

confidence: 83%

“…Only 2 (15.38%) out of 13 MRSA isolates were sensitive to amoxicillin whereas 84.62% isolates were found resistant. Similarly, higher resistance to amoxicillin was reported by Al-Zaidi and Al-Sulami (2013) [15] , Imran et al (2015) [16] , Nasution et al (2018) [17] and Shah et al (2019) [18] . Resistance of MRSA to beta-lactam antibiotics including penicillin, amoxicillin, methhicillin and cefoxitin is attributed to the presence of the mecA gene.…”

Section: Antibiogram Of Mrsamentioning

confidence: 52%

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Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Sannat¹,

Hirpurkar²,

Hazari³

et al. 2021

Pharma Innovation

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Present investigation was made to study antibiogram profile of Methicillin Resistant Staphylococcus aureus (MRSA) of animal origin. MRSA isolates were obtained from bovine mastitis and wound infection of animals using cultural isolation and were confirmed by S. aureus specific thermonuclease (nuc) and MRSA specific mecA gene in PCR assay. A total of 13 MRSA isolates were obtained and subjected to antibiotic sensitivity test using 10 numbers of selected antibiotics by disc diffusion test. MRSA isolates exhibited variable degree of sensitivity towards various antibiotics. MRSA isolates were detected 100% senisitive to linezolid and imipenem followed by clindamycin (92.31%), tetracycline (76.92%), vancomycin and gentamycin (53.85%). Isolates showed higher rate of resistance towards amoxicillin (84.62%) and penicillin (76.92%). All the isolates were found resistant to cefoxitin and methicillin. It can be concluded from present study that isolates harbouring mecA/ MRSA gene have tendency to exhibit resistant towards multidrug and treatment option can be explored by studying antibiogram of MRSA of particular region.

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Section: Antibiogram Of Mrsasupporting

confidence: 83%

Section: Antibiogram Of Mrsamentioning

confidence: 52%

Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Sannat¹,

Hirpurkar²,

Hazari³

et al. 2021

Pharma Innovation

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“…MRSA emerged as a S. aureus variant with methicillin resistance. Methicillin resistance in MRSA is mediated by the 78-kDa penicillin-binding protein PBP2 0 (or PBP2a), which has a very low affinity for β-lactam antibiotics and exhibits resistance not only to β-lactam antibiotics but also to other common antibiotics such as aminoglycosides, macrolides, cyclic peptides and fluoroquinolones (Miragaia 2018;Nasution et al 2018;Yehia et al 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Vancomycin has been considered the gold standard for treatment of serious MRSA infection, but the recent emergence of vancomycin‐resistant S. aureus necessitates the search for new antibiotics against MRSA (Nasution et al . 2018; Shah et al . 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The selection of an appropriate antibiotic for MRSA treatment is always a matter of concern. Vancomycin has been considered the gold standard for treatment of serious MRSA infection, but the recent emergence of vancomycin-resistant S. aureus necessitates the search for new antibiotics against MRSA (Nasution et al 2018;Shah et al 2019). Alternative approaches, such as antibiotic combination therapy or prolonged administration of highdose antibiotics, could be used to treat biofilm-associated recalcitrant pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Methanolic extract of Hemidesmus indicus root augments the antibacterial and antibiofilm activity of amoxicillin and clindamycin against methicillin-resistant Staphylococcus aureus of bovine origin

Sannat

Hirpurkar

Shakya

et al. 2022

Letters in Applied Microbiology

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The present study evaluated the antibacterial and antibiofilm activity of methanolic extract of Hemidesmus indicus root (MHIR) in combination with amoxicillin and clindamycin against biofilm‐forming methicillin‐resistant Staphylococcus aureus (MRSA) isolated from milk samples of mastitic cows. Microdilution susceptibility testing and microtitre plate assays were used to evaluate the in vitro efficacy of MHIR and antibiotic combinations against MRSA (n = 12). Furthermore, in vitro findings were validated in a murine model. Minimum inhibitory concentration and minimum biofilm inhibitory concentration of amoxicillin and clindamycin in combination with MHIR were significantly (P < 0·05) lower than when used alone against MRSA. In terms of antibacterial activity, MHIR showed additive interaction (fractional inhibitory concentrationindex >0·5–4) with amoxicillin and clindamycin against all the MRSA isolates, whereas MHIR synergizes (fractional biofilm inhibitory concentrationindex ≤0·5) the antibiofilm activity of amoxicillin and clindamycin against 58·33% and 83·33% of the MRSA isolates respectively. Amoxicillin/clindamycin in combination with MHIR significantly (P < 0·05) reduced disease activity score, and bacterial load and Gram‐positive spots in kidney and liver of MRSA‐infected mice. The combined efficacy of MHIR and amoxicillin/clindamycin was comparable to clindamycin alone but superior to amoxicillin alone. Hence, the combination of MHIR with amoxicillin/clindamycin is advocated in the treatment of MRSA‐associated infections.

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Amplifying PCR productivity and environmental sustainability through shortened cycling protocols

Pedlar,

Emery,

Warburton

2024

Biochimie

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Detection ofmeca gene from methicillin resistantstaphylococcus aureusisolates of north sumatera

Cited by 5 publications

References 14 publications

Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Methanolic extract of Hemidesmus indicus root augments the antibacterial and antibiofilm activity of amoxicillin and clindamycin against methicillin-resistant Staphylococcus aureus of bovine origin

Amplifying PCR productivity and environmental sustainability through shortened cycling protocols

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