Detection of <i>Helicobacter pylori</i> by Real‐Time <scp>PCR</scp> for 16s <scp>rRNA</scp> in Stools of NonInfected Healthy Children, Using <scp>ELISA</scp> Antigen Stool Test as the Gold Standard

George, Sergio; Mamaní, Nora; Lucero, Yalda; Torres, Juan Pablo; Farfán, Mauricio J.; Lagomarcino, Anne J.; Orellana, Andrea; O’Ryan, Miguel

doi:10.1111/hel.12318

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…Thus, HpSA using an ELISA is regarded as more convenient in children than the UBT . Furthermore, George et al . demonstrated, using a real‐time PCR in stools, that stool ELISA is a reliable method in characterizing H. pylori dynamics in apparently healthy children.…”

Section: Diagnosismentioning

confidence: 99%

“…Thus, HpSA using an ELISA is regarded as more convenient in children than the UBT. 30 Furthermore, George et al 31 29 Recently, antibody tests for detection of H. pylori-specific IgG by ELISA in urine have been tested on 806 junior and senior students in Japan. Its diagnostic accuracy was evaluated using UBT as a gold standard.…”

Section: Helicobacter Pylori Infection and Atopic Manifestationsmentioning

confidence: 99%

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Helicobacter pylori infection in children

2017

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Helicobacter pylori infection in children differs from that in adults, from the point of view of epidemiology, host response, clinical features, related diseases, and diagnosis, as well as treatment strategies. The prevalence of H. pylori infection, in both children and adults, is decreasing in the Western World as well as in some developing countries, which contrasts with the increase in childhood asthma and allergic diseases. Recurrent abdominal pain is not specific during H. pylori infection in children. The role of H. pylori infection and failure to thrive, children's growth, type I diabetes mellitus (T1DM) and celiac disease remains controversial. The main initial diagnosis is based on upper digestive endoscopy with biopsy-based methods. Nodular gastritis may be a pathognomonic endoscopic finding of childhood H. pylori infection. The infection eradication control is based on validated noninvasive tests. The main cause of treatment failure of H. pylori infection is its clarithromycin resistance. We recommend standard antibiotic susceptibility testing of H. pylori in pediatric patients prior to the initiation of eradication therapy. H. pylori treatment in children should be based on an evaluation of the rate of eradication in the local population, a systematic use of a treatment adapted to the susceptibility profile and a treatment compliance greater than 90%. The last meta-analysis in children did not show an advantage for sequential therapy when compared to a 14-day triple therapy. Finally, the high rate of antibiotic resistance responsible for therapy failure in recent years justifies the necessity of a novel vaccine to prevent H. pylori infection in children.

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Section: Diagnosismentioning

confidence: 99%

Section: Helicobacter Pylori Infection and Atopic Manifestationsmentioning

confidence: 99%

Helicobacter pylori infection in children

2017

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“…Furthermore, in another study that used a real-time PCR, it was shown that stool ELISA is a reliable method in characterizing H. pylori dynamics in apparently healthy children. Persistent infection was only detected in 2.7% of 36 children [84].…”

Section: Diagnosismentioning

confidence: 93%

Helicobacter pylori Infection in Pediatric Patients: Update on Diagnosis and Eradication Strategies

et al. 2018

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Helicobacter pylori infection is acquired mainly in childhood and remains an essential cause of peptic ulcer disease and gastric cancer. This article provides commentary on the last ESPGHAN/NASPGHAN guidelines and on publications made after the consensus conference of 2015. The majority of infected children are asymptomatic and pediatric studies do not support a role for H. pylori in functional disorders such as recurrent abdominal pain. The role of H. pylori infection in failure to thrive, children's growth, type I diabetes mellitus (T1DM), and celiac disease remains controversial. The diagnosis of infection should be based on upper-digestive endoscopy with biopsy-based methods. Eradication control after treatment should be based on validated non-invasive tests. Nodular gastritis is the main endoscopic finding of childhood H. pylori infection, but gastroduodenal erosions/ulcers are seen in some children, especially after 10 years of age. When indicated, eradication treatment should be given when good compliance is expected and based on the antimicrobial susceptibility profile.

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“…A real‐time PCR targeting the 16S rDNA in stools was used to complement the results of the stool antigen test (SAT). In the case of a SAT negative‐real‐time PCR positive result, a repeated PCR led to the conclusion of a persistent infection in 2.7% of the children …”

Section: Molecular Methodsmentioning

confidence: 99%

“…In the case of a SAT negative-real-time PCR positive result, a repeated PCR led to the conclusion of a persistent infection in 2.7% of the children. 24 This technique was also applied to look for H. pylori in an unusual niche, bronchoalveolar lavage of patients with lung cancer, and the result was positive in 11.5% of the patients. Contamination could occur by inhalation and contribute to the inflammation.…”

Section: Molecular Methodsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori infection

et al. 2017

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Important progress is being made in endoscopic methods which allow clinicians to predict the presence of Helicobacter pylori based on characteristics of gastric mucosa and to obtain targeted biopsies. There are also important developments in molecular methods with various techniques derived from standard PCR, applied both on gastric biopsies and stool specimens. Progress is being made in microfluidic systems to get a reliable diagnosis in a very short time. The interest of the C urea breath test has been confirmed as well as stool antigen tests. Attempts are being made to find biological markers of premalignant conditions by serology, other than pepsinogens.

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Detection of Helicobacter pylori by Real‐Time PCR for 16s rRNA in Stools of NonInfected Healthy Children, Using ELISA Antigen Stool Test as the Gold Standard

Cited by 7 publications

References 15 publications

Helicobacter pylori infection in children

Helicobacter pylori infection in children

Helicobacter pylori Infection in Pediatric Patients: Update on Diagnosis and Eradication Strategies

Diagnosis of Helicobacter pylori infection

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