Detection of ‘CandidatusMycoplasma haemobos’,Mycoplasma wenyoniiandAnaplasma phagocytophilumfrom cattle in England

Ayling, Roger D.; Bisgaard-Frantzen, S.; Adler, Andy; Blowey, Roger; Barlow, Alex; Millar, Michael; Burgt, G. M. van der

doi:10.1136/vr.100636

Cited by 24 publications

(13 citation statements)

References 19 publications

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“…(Tono et al, 2015), M. haemocanis and ‘ Candidatus Mycoplasma haematoparvum ’ (Aquino et al, 2016). However, molecular evidence of ‘ Candidatus M. haemobos ’ has only been reported in Asia, America and Europe in cattle (Ayling et al, 2012; Girotto et al, 2012; Hoelzle et al, 2011; McFadden et al, 2016; Meli et al, 2010; Tagawa et al, 2008) and in dogs from northern Australia (Hii et al, 2012). The clinical significance of this organism in dogs remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Detection and identification of blood-borne infections in dogs in Nigeria using light microscopy and the polymerase chain reaction

Happi

Toepp

Ugwu

et al. 2018

Veterinary Parasitology: Regional Studies and Reports

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Many sick dogs brought to the University of Ibadan Veterinary Teaching Hospital (UIVTH) are infested with ticks and are anemic. Up until recently, light microscopy (LM) has been the only available means used for detection of blood-borne infections. In other parts of the world, PCR-based assays been used as a gold standard for accurate diagnosis of blood-borne infections. In this study, we used LM and broad-spectrum rRNA gene PCR-based assays on 116 blood samples from dogs brought to the UIVTH for detection of the 18S rRNA gene of Babesia and the 16S rRNA genes of Ehrlichia and hemotropic mycoplasmas. The relationship between clinicopathological findings and PCR results was evaluated. Age, sex, presence of ticks, anemia, co-infection status, and fever were also assessed in relation to PCR positivity to determine the risk factors using stepwise logistic regression analyses. Light microscopic examination revealed an overall prevalence of infection of 14.7% (17/116). Organisms detected were Babesia canis (3.5%), Ehrlichia canis (10.3%) and Trypanosoma congolense (0.9%) and a single coinfection with Babesia canis and Ehrlichia canis (0.9%). PCR analysis revealed 89/116 (76.7%) positive samples. Infections with 1, 2 and 3 infectious agents occurred in 49 (55.1%), 36 (40.4%) and 4 (4.5%) samples, respectively. Specifically, among the 89 PCR positive samples, Babesia spp. (85.4%) was the most abundant infection followed by Ehrlichia spp. (46.1%) and hemoplasmas (13.5%). Sequencing of PCR products identified two samples (1.7%) that contained Hepatozoon canis DNA. Sequencing of hemoplasma positive samples identified ‘Candidatus Mycoplasma haemobos’ in 0.8% of dogs. Using PCR, a 5-fold higher prevalence of blood-borne infections was found in the dogs (76.7%, 89/116) than with LM (14.7%, 17/116) alone” Dogs between 1 and 12 months were the most frequently infected with multiple agents (47.2% double and 50.0% triple infections). Male dogs had the highest prevalence of infection (80.4%) and more triple infections (75.0%). A total of 57.3% of infected dogs were anemic. Anemic dogs were 2.77 times more likely to test positive for Ehrlichia spp. (OR: 2.77 95% CI: 1.25–6.16) and dogs with ticks were 3.6 times more likely to test positive for hemoplasmas (OR = 3.60 95% CI: 1.05–12.38). This study underscores the abundance of blood-borne infections in dogs in Ibadan, Nigeria, which is underestimated using light microscopy. This is also the first evidence of existence of ‘Candidatus Mycoplasma haemobos’ in a dog in Nigeria and in Africa. Consequently there is a need for molecular diagnostic facilities for routine screening of sick animals, as multiple infections were not found by light microscopy.

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Section: Discussionmentioning

confidence: 99%

Detection and identification of blood-borne infections in dogs in Nigeria using light microscopy and the polymerase chain reaction

Happi

Toepp

Ugwu

et al. 2018

Veterinary Parasitology: Regional Studies and Reports

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“…We found that hemoplasmas are located outside the erythrocyte as has been reported as hemoplasmas, as is shown in Figure 4. Due to hemoplasmas can cause anemia, loss of weight, fever, edema, reproductive problems, mastitis and jaundice, their detection is the first step to characterize the agents present in the infected host (Ayling et al, 2012). Today, the use of molecular markers and their PCR amplification is the most accessible and common method to detect the presence of pathogens.…”

Section: Propidium Iodide Staining Of Hemoplasmasmentioning

confidence: 99%

Molecular detection of bovine hemotrophic mycoplasmas in Mexico

Quiroz-Castañeda¹,

Aragón²,

Diaz³

et al. 2020

RECEIN

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The presence of hemoplasmas Candidatus Mycoplasma haemobos and Mycoplasma wenyonii that infect bovine cattle has been reported during the last years. Hemoplasmas may affect animal health either alone or in coinfections with other microorganisms, resulting in anemia and other clinical signs. In Mexico, only Ca. M. haemobos has been detected in cattle; in this work, we report for the first time in our country the presence of M. wenyonii in animals from different geographical sources amd we detected both hemoplasmas by duplex PCR. Also, by single end-point PCR, we found Ca. M. haemobos and M. wenyonii in 96% and 96.29% of the blood samples, respectively. Both hemoplasmas were detected in 50% of the samples analyzed, which suggest that the duplex PCR developed in this work might improve if some modifications are performed. This molecular detection method will provide valuable information to know the health condition of national cattle to prevent pathogen dispersion.

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“…Recently, A. phagocytophilum has been reported in ruminants and I. ricinus, removed from humans, in the east Black Sea region of Turkey (10,11,12). Tick-born fever (TBF), which is caused by the prototype of Anaplasma phagocytophilum as a disease in cattle in England (13).…”

Section: Introductionmentioning

confidence: 99%