Detection of<i>Candida albicans</i>mRNA in Archival Histopathology Samples by Reverse Transcription-PCR

Beggs, Kyle T.; Holmes, Ann R.; Cannon, Richard D.; Rich, Alison M.

doi:10.1128/jcm.42.5.2275-2278.2004

Cited by 10 publications

(8 citation statements)

References 25 publications

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“…To increase the sensitivity of mRNA detection, the number of PCR cycles was also increased from 30 to 45 cycles and the amount of template (CaADH2 cDNA) used for PCR amplification was doubled (to twice that used for detection of Ca18S rRNA and CaADH1 mRNA). Under this condition, only 3 of the 10 samples tested positive, suggesting differential expression for CaADH2 and similar results had also been reported by Beggs et al (12).…”

Section: Discussionsupporting

confidence: 90%

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Detection of Candida albicans ADH1 and ADH2 mRNAs in human archival oral biopsy samples

Bakri

Cannon

Holmes

et al. 2014

J Oral Pathology Medicine

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Candida albicans was the predominant species in the lesions diagnosed as CHC, and the presence of C. albicans in CHC lesions was associated with a high expression of C. albicans ADH1 mRNA. There was no association between the presence of Candida and malignant transformation in the cases examined; however, the number of cases was limited and further studies are needed to further elucidate the role of C. albicans ADH1 in the pathogenesis of oral squamous cell carcinoma.

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Section: Discussionsupporting

confidence: 90%

“…Under this condition, only 3 of the 10 samples tested positive, suggesting differential expression for CaADH2 and similar results had also been reported by Beggs et al. .…”

Section: Discussionsupporting

confidence: 90%

Detection of Candida albicans ADH1 and ADH2 mRNAs in human archival oral biopsy samples

Bakri

Cannon

Holmes

et al. 2014

J Oral Pathology Medicine

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“…Relative quantification of gene expression was performed using the comparative C T method (sequence detection systems chemistry guide; Applied Biosystems). The most stable housekeeping genes, EFB1 and ACT1 (11), across the sample states were chosen using the statistical technique described by Vandesompele et al (73). The internal normalization factor was the geometric mean of the stable housekeeping genes (73).…”

Section: Methodsmentioning

confidence: 99%

A Small Subpopulation of Blastospores in Candida albicans Biofilms Exhibit Resistance to Amphotericin B Associated with Differential Regulation of Ergosterol and β -1,6-Glucan Pathway Genes

Khot

Suci

Miller

et al. 2006

Antimicrob Agents Chemother

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The resistance of Candida albicans biofilms to a broad spectrum of antimicrobial agents has been well documented. Biofilms are known to be heterogeneous, consisting of microenvironments that may induce formation of resistant subpopulations. In this study we characterized one such subpopulation. C. albicans biofilms were cultured in a tubular flow cell (TF) for 36 h. The relatively large shear forces imposed by draining the TF removed most of the biofilm, which consisted of a tangled mass of filamentous forms with associated clusters of yeast forms. This portion of the biofilm exhibited the classic architecture and morphological heterogeneity of a C. albicans biofilm and was only slightly more resistant than either exponential-or stationary-phase planktonic cells. A submonolayer fraction of blastospores that remained on the substratum was resistant to 10 times the amphotericin B dose that eliminated the activity of the planktonic populations. A comparison between planktonic and biofilm populations of transcript abundance for genes coding for enzymes in the ergosterol (ERG1, -3, -5, -6, -9, -11, and -25) and ␤-1,6-glucan (SKN and KRE1, -5, -6, and -9) pathways was performed by quantitative RT-PCR. The results indicate a possible association between the high level of resistance exhibited by the blastospore subpopulation and differential regulation of ERG1, ERG25, SKN1, and KRE1. We hypothesize that the resistance originates from a synergistic effect involving changes in both the cell membrane and the cell wall.

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“…Numerous studies had shown a positive correlation between epithelial dysplasia, oral squamous cell carcinoma and elevated level of Candida species in the oral cavity [24][25][26][27]. It had been demonstrated that oral candidosis may play a significant role in malignant transformation generally.…”

Section: Experimental Part Materials and Methodsmentioning

confidence: 99%

Preventive andTherapeutic Effects of Chlorhexidine Containing Varnish on Candida Biofilm

Mensch¹,

Pongrácz²,

Nagy³

et al. 2018

Rev. Chim.

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The aim of the study was to assess the preventive and therapeutic efficacy of a sustained-release varnish (SRV) Cervitec Plus� compared with Corsodyl� mouthwash against Candida albicans, Candida parapsilosis, Candida tropicalis and Candida glabrata on acrylate surfaces. Nystatin as the positive control, and sterile saline solution as the negative control, were used. To examine the preventive efficacy of the agents, Candida biofilms were developed in Sabouraud liquid medium, and mature biofilms were treated with the antifungal. To investigate the preventive efficacy, the agents were applied to the wells of the plates and the biofilms were grown in the treated wells. To determine the metabolic activity of biofilms, the XTT-assay was used. In the case of C. albicans and C. parapsilosis, the most effective therapeutic product was Corsodyl� followed by Cervitec Plus� and Nystatin. The differences were statistically significant when Corsodyl� was compared to Nystatin ( p � 0.05). In relation to C. tropicalis and C. glabrata, Corsodyl� showed the greatest therapeutic efficacy followed by Nystatin and Cervitec Plus�. For both species, the results of Corsodyl� and Cervitec Plus� showed statistically significant difference ( p � 0.05). The most effective preventive product for each examined Candida species was Nystatin, followed by Cervitec Plus� and Corsodyl�. Significant differences were between Nystatin and Corsodyl� (p � 0.05). The preventive and therapeutic efficacy of Cervitec Plus� and Corsodyl�, against the examined Candida species, was detected. Because of the disadvantageous side-effects of Nystatin, Cervitec Plus� can be considered as an alternative preventive agent against Candida species.

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Detection ofCandida albicansmRNA in Archival Histopathology Samples by Reverse Transcription-PCR

Cited by 10 publications

References 25 publications

Detection of Candida albicans ADH1 and ADH2 mRNAs in human archival oral biopsy samples

Detection of Candida albicans ADH1 and ADH2 mRNAs in human archival oral biopsy samples

A Small Subpopulation of Blastospores in Candida albicans Biofilms Exhibit Resistance to Amphotericin B Associated with Differential Regulation of Ergosterol and β -1,6-Glucan Pathway Genes

Preventive andTherapeutic Effects of Chlorhexidine Containing Varnish on Candida Biofilm

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