Detection of <i>ALK</i> Gene Rearrangement in Cell-free RNA from Lung Cancer Malignant Pleural Effusion

Chu, Mingliang; Zhu, Yanqiu; Hu, Jianjun; Zhang, Zhuxue; Luo, Meng; Ma, Xiaobo

doi:10.1155/2020/6124106

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…In the current study, we did not perform a gene panel test using CB specimens. However, we were able to obtain a large number of malignant cells in the CB specimens, suggesting that the testing of genetic alterations is possible, as reported in cases of lung cancer [ 33 , 34 ]. It is necessary to evaluate whether the CB samples would be suitable for NGS in a future study.…”

Section: Discussionmentioning

confidence: 71%

Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis

Okuno

Mukai

Iwata

et al. 2022

Cancers

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The specimen collection and subsequent pathological diagnosis of malignant biliary stricture (MBS) are difficult. This study aimed to determine whether the cell block (CB) method using overnight-stored bile is useful in the diagnosis of MBS. This trial was a single-arm prospective study involving a total of 59 patients with suspected MBS. The primary endpoint was cancer detectability and accuracy using the CB method, and a comparison with the detectability and accuracy achieved with bile cytology was made. The immunohistochemical sensitivity for maspin and p53 was also investigated in the CB and surgical specimens. We were able to collect bile from all 59 patients, and 45 of these patients were clinically diagnosed with MBS. The cancer detectability using the CB method (62.2%) was significantly higher than that using cytology (37.8%) (p = 0.0344). When CB was combined with biopsy, the rates of cancer detectability (75.6%) and accuracy (81.4%) increased. In eight patients who received surgical therapy, maspin- and p53-immunohistochemistry was applied to the surgical and CB specimens, and cancer cells in both specimens showed positive cytoplasmic and nuclear staining for maspin and nuclear staining for p53. The CB method is, thus, useful for detecting malignancy (UMIN000034707).

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Section: Discussionmentioning

confidence: 71%

Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis

Okuno

Mukai

Iwata

et al. 2022

Cancers

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show abstract

Section: Discussionmentioning

confidence: 87%

“…For the pleural effusion cfRNA detection, previous study showed that the pleural effusion cfRNA can successfully be extracted and achieving a 100% concordance rate of ALK fusion detection compared with cell blocks by PCR method. 29 However, this study did not obtain matched tissue specimens as reference. Another study also showed the success rates of pleural effusion cell free total nucleic acid extraction and NGS sequencing have been verified to be higher than that of cell blocks by Xiang et al 22 In this study, we confirmed that fusions could be successfully detected in the cfRNA derived from MPE supernatant by using a PCR-based method, achieving a concordance rate of 97.9% (46/47) with paired tissue samples.…”

Section: Discussionmentioning

confidence: 92%

Multigene PCR using both cfDNA and cfRNA in the supernatant of pleural effusion achieves accurate and rapid detection of mutations and fusions of driver genes in patients with advanced NSCLC

Chen

Liu

et al. 2021

Cancer Medicine

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“…Previously, up to 7% EML4/ALK fusion rate was found in patients with brain metastases [ 55 ]. The ALK gene is often fused with EML4 gene in NSCLC, leading to continuous expression of ALK and the activation of downstream PI3K/Akt/MAPK signal pathway and occurrence of tumors [ 56 , 57 ]. In this study, we found that ALK mutation is associated with pathological features of tumors (lymph node metastasis, TNM stage, differentiation) as well as mortality.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of Beclin 1, EGFR and ALK in non-squamous non-small cell lung cancer

Wan

Wang

et al. 2022

Discov Onc

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Non-small cell lung cancer (NSCLC) is one of the most malignant tumors. The study was carried out to investigate the prognostic value of Beclin 1, EGFR and ALK for this cancer. Patients diagnosed with non-squamous NSCLC and admitted to our hospital from January 2011 to September 2016 were analyzed. Expression of Beclin 1 and mutation of EGFR and ALK were assessed using polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) and analyzed for their relationship with demographic and clinical characteristics of the patients. Multivariate Cox regression models were applied to analyze the risk factors associated with survival and receiver response curves (ROC) were plotted to determine the prognostic value of Beclin 1, EGFR and ALK for patients with non-squamous NSCLC. Compared with adjacent normal tissue, Beclin 1 expression was elevated in the cancer tissue significantly; assessments of EGFR and ALK mutations showed that out of the 480 patients, 233 (48.5%) and 75 (12.6%) patients had EGFR and ALK mutations. Univariate analysis revealed that Beclin 1 level, EGFR and ALK mutations were associated with lymph node metastasis, TNM stage, tumor differentiation and prognosis, but not with gender, age and smoking status. The Kaplan–Meier survival analysis indicated that low Beclin 1 expression and positive EGFR and ALK rearrangements were associated with higher survival rate and longer progress-free survival (PFS). Multivariate Cox regression analysis showed that Beclin 1, EGFR, ALK mutations, tumor differentiation grade, TNM stage and lymph node metastasis were independently associated with PFS. ROC analysis showed that Beclin 1, EGFR and ALK were significant predictors for PFS; the areas under curve (AUC) for Beclin 1, EGFR and ALK were 0.812 (P = 0.018, cut-off value: 1.2), 0.781 (P = 0.011, cut-off value: 15%) and 0.722 (P = 0.010, cut-off value: 11%), respectively, suggesting that they have significant prognostic value for lung cancer patients. Our data indicate that Beclin 1, EGFR and ALK genes are associated with the prognosis of patients with non-squamous NSCLC. High Beclin 1 expression and negative EGFR and ALK mutations predict a poor prognosis with PFS.

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Detection of ALK Gene Rearrangement in Cell-free RNA from Lung Cancer Malignant Pleural Effusion

Cited by 4 publications

References 12 publications

Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis

Evaluation of the Cell Block Method Using Overnight-Stored Bile for Malignant Biliary Stricture Diagnosis

Multigene PCR using both cfDNA and cfRNA in the supernatant of pleural effusion achieves accurate and rapid detection of mutations and fusions of driver genes in patients with advanced NSCLC

Prognostic value of Beclin 1, EGFR and ALK in non-squamous non-small cell lung cancer

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