Detection of hydrolyzed zearalenone (HZEN) as intermediate in Zearalenone catabolism of Clonostachys rosea

Fruhauf, S.; Kunz-Vekiru, Elisavet; Schatzmayr, Gerd; Moll, Wulf-Dieter

doi:10.1016/j.nbt.2012.08.464

Cited by 3 publications

(6 citation statements)

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“…Deoxynivalenol (DON) and deepoxy-deoxynivalenol (DOM-1) were dissolved in sterile water to obtain a 5 mM stock solution. Zearalenone (ZEN) and hydrolyzed-zearalenone (HZEN) were dissolved in sterile water and DMSO (1:1 dilution ratio) to obtain a 5 mM stock solution ( 46 ). Stock solutions were stored in aliquots at -20°C.…”

Section: Methodsmentioning

confidence: 99%

Effect of DON and ZEN and their metabolites DOM-1 and HZEN on B cell proliferation and antibody production

Pierron,

Kleber,

Mayer

et al. 2024

Front. Immunol.

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IntroductionThe mycotoxins deoxynivalenol (DON) and zearalenone (ZEN), produced by Fusarium fungi, are frequently found in the cereal-rich diet of pigs and can modulate the immune system. Some enzymes or bacteria present in the digestive tract can de-epoxydize DON to deepoxy-deoxynivalenol (DOM-1) and biotransform ZEN into hydrolyzed ZEN (HZEN). The effects of these metabolites on immune cells, particularly with respect to the vaccine responses, are poorly documented. The aim of this study was to address the impact of DON and ZEN and their respective derivatives, on proliferation, and antibody production of porcine B cells in vitro.MethodsPeripheral blood mononuclear cells (PBMCs), isolated from healthy pigs, were stimulated with the Toll-like receptor (TLR) 7/8-agonist Resiquimod (R848) or the TLR/1/2-agonist Pam3Cys-SKKKK in combination with DON [0.1-1.6 µM] or DOM-1 [1.6 µM and 16 µM] and ZEN [2.5-40 µM] or HZEN [40 µM].ResultsA strong decrease in B-cell proliferation was observed at DON concentrations equal to or exceeding 0.8 µM and at ZEN concentrations equal to or exceeding 20 µM. Treatment with 1.6 µM DON or 40 µM ZEN led to almost a complete loss of live CD79α+ B cells. Moreover, CD21 expression of proliferating IgG+ and IgM+ B-cell subsets was decreased at DON concentrations equal to and exceeding 0.4 µM and at ZEN concentrations equal to or exceeding 10 µM. ELISpot assays revealed a decrease of IgG-secreting B cells at concentrations of and exceeding 0.4 µM and at ZEN concentrations equal to and exceeding 10 µM. ELISA assays showed a decrease of IgM, IgG, and IgA secretion at concentrations equal to or exceeding 0.4 µM DON. ZEN reduced IgM secretion at 20-40 µM (both R848 and Pam3Cys-SKKKK), IgG secretion at 40 µM (both R848 and Pam3Cys-SKKKK) and IgA secretion at 20-40 µM.DiscussionOur in vitro experiments show that while DON and ZEN impair immunoglobulin production and B-cell proliferation, this effect is abrogated by HZEN and DOM-1.

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Section: Methodsmentioning

confidence: 99%

Effect of DON and ZEN and their metabolites DOM-1 and HZEN on B cell proliferation and antibody production

Pierron,

Kleber,

Mayer

et al. 2024

Front. Immunol.

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“…Screening of soil samples reported in the study [53] revealed an R. erythropolis PFA D8-1 strain able to convert ZEN via the cleavage of the lactone ring with a formation of hydrolyzed ZEN (HZEN). The loss of estrogenicity in the final product was confirmed by the MCF7 bioassay, as well as by the test with an estrogen reporter yeast strain YZHB817.…”

Section: Zen-transforming Bacteriamentioning

confidence: 99%

“…Finally, ZenA, a zearalenone hydrolase derived from Rhodococcus erythropolis PFA D8-1, was shown to possess a higher substrate affinity and catalytic rate than ZHD101, and successfully cloned in E. coli. [53]. The enzyme manufactured by BIOMIN Holding GmbH (Getzersdorf, Austria) with the commercial name Zenzyme ® was successfully tested on cows, pigs, chickens, and rainbow trout [79,80].…”

Section: Zen-transforming Microbial Enzymesmentioning

confidence: 99%

“…Another possible way is a screening of microbial strains belonging to the genera that provided some effective mycotoxin-transforming strains. For example, a number of ZEN-degrading strains were revealed among the genus Rhodococcus [50][51][52][53], and a number of DON-degrading strains were reported among the genera Devosia [115,[117][118][119][120] and Nocardioides [120][121][122][123]. In the case of ZEN, good screening results may be also obtained using some biotests providing an evaluation of the estrogenic potential of ZEN transformation products [50].…”

Section: Promising Directions Of Studies Intended To Search For Poten...mentioning

confidence: 99%

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Post-Harvest Prevention of Fusariotoxin Contamination of Agricultural Products by Irreversible Microbial Biotransformation: Current Status and Prospects

Statsyuk

Popletaeva

Щербакова

2023

BioTech

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Biological degradation of mycotoxins is a promising environmentally-friendly alternative to chemical and physical detoxification methods. To date, a lot of microorganisms able to degrade them have been described; however, the number of studies determining degradation mechanisms and irreversibility of transformation, identifying resulting metabolites, and evaluating in vivo efficiency and safety of such biodegradation is significantly lower. At the same time, these data are crucial for the evaluation of the potential of the practical application of such microorganisms as mycotoxin-decontaminating agents or sources of mycotoxin-degrading enzymes. To date, there are no published reviews, which would be focused only on mycotoxin-degrading microorganisms with the proved irreversible transformation of these compounds into less toxic compounds. In this review, the existing information about microorganisms able to efficiently transform the three most common fusariotoxins (zearalenone, deoxinyvalenol, and fumonisin B1) is presented with allowance for the data on the corresponding irreversible transformation pathways, produced metabolites, and/or toxicity reduction. The recent data on the enzymes responsible for the irreversible transformation of these fusariotoxins are also presented, and the promising future trends in the studies in this area are discussed.

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“…It relies on its unique structure to regulate the anti-thrombin AT-III, inhibits the activity of coagulation factor IIa and Xa, and achieves specific stages that interfere with the coagulation process. Fruhauf et al (2012) reported that yeast β-glucan can adsorb a variety of mycotoxins including zearalenone and aflatoxin [127]. Xu et al (2015) and Zhang et al (2006) reported that the unique three-dimensional structure of yeast β-glucan has a good adsorption effect on mycotoxins [128,129].…”

Section: Other Biological Activitymentioning

confidence: 99%

Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review

Yuan

Lan

et al. 2019

Molecules

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β-Glucan exhibits many biological activities and functions such as stimulation of the immune system and anti-inflammatory, anti-microbial, anti-infective, anti-viral, anti-tumor, anti-oxidant, anti-coagulant, cholesterol-lowering, radio protective, and wound healing effects. It has a wide variety of uses in pharmaceutical, cosmetic, and chemical industries as well as in food processing units. However, due to its dense triple helix structure, formed by the interaction of polyhydroxy groups in the β-d-glucan molecule, it features poor solubility, which not only constrains its applications, but also inhibits its physiological function in vivo. One aim is to expand the applications for modified β-glucan with potential to prevent disease, various therapeutic purposes and as health-improving ingredients in functional foods and cosmetics. This review introduces the major modification methods required to understand the bioactivity of β-glucan and critically provides a literature survey on the structural features of this molecule and reported biological activity. We also discuss a new method to create novel opportunities to exploit maximally various properties of β-glucan, namely ultrasound-assisted enzymatic modification.

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Detection of hydrolyzed zearalenone (HZEN) as intermediate in Zearalenone catabolism of Clonostachys rosea

Cited by 3 publications

References 0 publications

Effect of DON and ZEN and their metabolites DOM-1 and HZEN on B cell proliferation and antibody production

Effect of DON and ZEN and their metabolites DOM-1 and HZEN on B cell proliferation and antibody production

Post-Harvest Prevention of Fusariotoxin Contamination of Agricultural Products by Irreversible Microbial Biotransformation: Current Status and Prospects

Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review

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