Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis

Zhang, Megan Q.; El‐Mofty, Samir K.; Dávila, Rosa M.

doi:10.1002/cncr.23348

Cited by 103 publications

(96 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Markers of HPV-associated tumors have been utilized in recent studies addressing this problem. Evaluation of fine-needle aspiration biopsies of neck metastases by in situ hybridization for HPV (Begum et al, 2007;Zhang et al, 2008) and by immunohistochemistry for p16 overexpression (Begum et al, 2007) have been shown to be predictive of an oropharyngeal primary tumor (Fig. 2).…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: Recent Evidence and Clinical Implications

2009

View full text Add to dashboard Cite

Over the past 20 years, high-risk human papillomavirus (HPV) infection has been established as a risk factor for developing head and neck squamous cell carcinoma, independent of tobacco and alcohol use. In particular, HPV is strongly associated with the development of oropharyngeal cancer and a small minority of oral cavity cancers. In this review, we summarize what is currently known about the biology of HPV, the mechanisms by which it effects malignant transformation, and the potential impact of HPV status on the clinical management of persons with head and neck cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: Recent Evidence and Clinical Implications

2009

View full text Add to dashboard Cite

show abstract

“…Densely eosinophilic cytoplasm characteristic of K SCC is absent or sparse. However, FNA classification as NK or K does not always correlate with histology [23]. This may be due to a subset of poorly differentiated K SCCs lacking overtly keratinized cells and thus mimicking an NK SCC.…”

Section: Squamous Cell Carcinoma Of Unknown Primary In the Era Of Hummentioning

confidence: 96%

“…NK features typical of HPV-related oropharyngeal SCC have been described on FNA [23]. These include cohesive clusters of cells that are relatively monomorphic and have high nuclear to cytoplasmic ratios.…”

Section: Squamous Cell Carcinoma Of Unknown Primary In the Era Of Hummentioning

confidence: 99%

Approach to Metastatic Carcinoma of Unknown Primary in the Head and Neck: Squamous Cell Carcinoma and Beyond

Chernock

Lewis

2015

Head and Neck Pathol

View full text Add to dashboard Cite

show abstract

“…We have recently shown that HPV positive carcinomas in metastatic cervical lymph nodes could also be reliably identified, in fine needle aspiration (FNA) biopsies, by characteristic non-keratinizing cytologic features [30].…”

Section: Discussionmentioning

confidence: 99%

Histologic Identification of Human Papillomavirus (HPV)-Related Squamous Cell Carcinoma in Cervical Lymph Nodes: A Reliable Predictor of the Site of an Occult Head and Neck Primary Carcinoma

El‐Mofty

Zhang

Dávila

2008

Head and Neck Pathol

Self Cite

View full text Add to dashboard Cite

Objective Patients with head and neck squamous cell carcinoma (SCC) often present with cervical lymph node metastasis. Occasionally the primary tumor site remains unknown even after thorough investigation. Management of such cases is problematic and may result in over-treatment and consequent increased morbidity. High risk HPV has been advocated recently as an important etiologic factor for a subset of head and neck SCC. These are believed to have a special predilection for the oropharyngeal tonsils and are characterized by nonkeratinizing basaloid morphology, and a strong reactivity to p16 immunostain. Identifying HPV-related SCC in the lymph nodes may thus provide a means for localizing the primary tumor site. Design Ninety-three cases of SCC metastatic to the neck from known primary tumors were classified morphologically into conventional keratinizing SCC (KSCC) and non-keratinizing SCC (NKCa). In situ hybridization (ISH) for high risk HPV as well as immunostaining for p16 were performed on all metastsatic and primary tumors. Results Of the 93 cases of metastatic carcinomas 32 were oropharyngeal, 35 oral, and 26 arose in the laryx/hypopharynx. Twenty-three cases were found to be HPV+ by ISH, of which 22/23 had oropharyngeal origin (P \ 0.0001), with 95.7% sensitivity and 85.7% specificity. Twenty-one of these HPV+ oropharyngeal tumors were NKCa (P \ 0.0001). The remaining case showed overlapping NKCa/KSCC hybrid morphology. All NKCa were HPV+ and stained diffusely and strongly with p16 antibodies. Conclusion We have demonstrated that HPV status of the lymph node metastasis can be assessed not only by ISH and p16 immunoreactivity but also histomorphologically. In addition, a positive microscopic identification of HPVrelated carcinoma is a reliable predictor of oropharyngeal origin.

show abstract

Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis

Cited by 103 publications

References 14 publications

Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: Recent Evidence and Clinical Implications

Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: Recent Evidence and Clinical Implications

Approach to Metastatic Carcinoma of Unknown Primary in the Head and Neck: Squamous Cell Carcinoma and Beyond

Histologic Identification of Human Papillomavirus (HPV)-Related Squamous Cell Carcinoma in Cervical Lymph Nodes: A Reliable Predictor of the Site of an Occult Head and Neck Primary Carcinoma

Contact Info

Product

Resources

About