Detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction

Hoshikawa, Tomohide; Nakajima, Takashi; Uhara, Hisashi; Gotoh, Masahiro; Shimosato, Yukío; Tsutsumi, Kouichiro; Ono, Isamu; Ebihara, Satoshi

doi:10.1288/00005537-199006000-00018

Cited by 88 publications

(41 citation statements)

References 16 publications

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“…A link between HPV infection and cervical cancer has been demonstrated in over 99% of specimens (Walboomers et al, 1999). Recent investigations, on the other hand, do not support HPV as an important factor in the pathogenesis of laryngeal epithelial lesions (Brandwein et al, 1993), although the prevalence of HPV in laryngeal carcinomas has been found to range from 5 to 54.1% (Hoshikawa et al, 1990;Perez-Ayala et al, 1990;Brandwein et al, 1993). The aetiology of laryngeal cancer is more likely to be related to cigarette smoking, alcohol abuse and exposure to other extrinsic irritants.…”

Section: Discussionmentioning

confidence: 99%

Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions

et al. 2003

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Histological classification of laryngeal epithelial lesions is highly subjective, and methods of cytological detection are not well developed. Improved determination of aberrant cell cycle entry may allow increased objectivity in histological assessment and enable the development of less invasive diagnostic cytology tests. Sections of normal larynx (n ¼ 10), laryngeal dysplasia (n ¼ 20) and laryngeal squamous cell carcinoma (SCC) (n ¼ 10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. Expression patterns were compared using double labelling confocal microscopy. There was a correlation between Mcm-2 and Ki67 labelling indices (r ¼ 0.93; 95% CI [0.84, 0.97]) and both markers showed increased expression from normal epithelium to SCC (Mcm-2, P ¼ 0.001; Ki67, P ¼ 0.0002). Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. Clusters of Mcm-2/5-positive cells were present in cytological preparations from SCC, but not from those showing atypical hyperplasia or inflammation in non-neoplastic tissue. Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions. Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

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Section: Discussionmentioning

confidence: 99%

Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions

et al. 2003

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“…Cerca de 40% dos carcinomas espinocelulares invasivos de laringe apresentam o tipo 16 do HPV 27 . Não se pode, contudo, afirmar que a transformação maligna na papilomatose ocorre exclusivamente associada à presença do HPV, ou se trata-se de um fenômeno paralelo associado a outros estímulos carcinógenos a que a laringe está exposta.. Co-fatores capazes de modificar genes celulares e/ou controladores intracelulares como álcool, tabaco, Vírus Herpes Simplex e Citomegalovírus podem agir como iniciadores responsáveis pela oncogênese 8,28,29 .…”

Section: Discussionunclassified

Papilomatose laríngea recorrente: experiência de 10 anos

Filho

Simoceli

Imamura

et al. 2003

Rev. Bras. Otorrinolaringol.

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Recurrent laryngeal papillomatosis: a 10-year experience Resumo / SummaryInt rodução: Os papilomas de vias respiratórias são tumores benignos, de caráter recorrente e progressivo, induzidos por vírus. Sua etiologia está relacionada com infecções por papilloma virus humano, sendo descritas duas formas clínicas: juvenil e adulta. Objetivo: Comparar as duas formas de apresentação, juvenil e adulta, observando se há diferenças epidemiológicas ou clínicas entre elas. Desenho do Estudo: Coorte histórica. Material e Método: Realizou-se estudo dos pacientes atendidos na Clínica Otorrinolaringológica do HC-FMUSP com diagnóstico de papilomatose laríngea entre 1990 e 1999. Cinqüenta e um pacientes foram identificados, mas sete não confirmaram o diagnóstico. Assim, 44 indivíduos foram a base desta revisão, sendo 47,72% da forma juvenil e 52,27% da forma adulta. Resultados: Não houve predomínio entre os sexos (p=0,98). A idade média de início dos sintomas foi de 5,3 anos na forma juvenil e 42,6 anos na forma adulta. A dispnéia foi mais prevalente na forma juvenil (p=0,03). A taxa de recidiva geral foi de 66%, sendo de 76,2% na forma juvenil e 56,5% na forma adulta (p=0,17). A incidência de recidiva precoce (<3 meses) foi maior na forma juvenil (p<0,001). Observamos 13% de transformação maligna na forma adulta da doença. Conclusões: Na forma juvenil, ocorreram recidivas precoces associadas a quadros de dispnéia, necessitando de múltiplas intervenções. Na forma adulta, também observamos alta taxa de recidivas, com índice elevado de transformação maligna. Tratamentos que aumentem o tempo entre as recidivas se fazem necessários nas duas formas de apresentação da doença.

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“…Very rarely, squamous cell carcinomas can arise in pre-existing laryngeal papillomas after a long latency period. Surprisingly, most of the HPV-positive laryngeal carcinomas contain HPV-16 (Hoshikawa et al, 1990;Perez-Ayala et al, 1990;McCuUough & McNicol, 1991), but the malignant progression of laryngopharyngeal lesions containing HPV-6 or-11 has also been reported (Zarod et al, 1988;Lindeberg et al, 1989). However, duplications and insertions are often found in the upstream regulatory region (URR) of low-risk HPVs present in high-grade lesions (Boshart & zur Hausen, 1986;Rando et al, 1986a;Kasher & Roman, 1988;DiLorenzo et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Classification of Human Papillomaviruses: Correlation With Clinical Manifestations

Ranst

Kaplan²,

Burk

1992

Journal of General Virology

201

104

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Human papillomaviruses (HPVs) are a heterogeneous group of small dsDNA viruses which cause a variety of proliferative epithelial lesions at specific anatomical sites. Although more than 65 different virus types have been cloned and characterized, no uniform classification system exists. In order to classify HPV DNA types, phylogenetic trees were constructed based on nucleotide sequence alignments using parsimony and distance matrix algorithms. The resulting phylogenetic trees provide a classification of the HPVs into specific groups encompassing the known tissue tropism and oncogenic potential of each HPV type. The implications of a phylogenetie taxonomy on the diagnostic detection of HPVs and the concept of different HPV species are discussed.

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Detection of human papillomavirus dna in laryngeal squamous cell carcinomas by polymerase chain reaction

Cited by 88 publications

References 16 publications

Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions

Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions

Papilomatose laríngea recorrente: experiência de 10 anos

Phylogenetic Classification of Human Papillomaviruses: Correlation With Clinical Manifestations

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