Detection of human papillomavirus and expression of osteopontin in cervical cancer specimens

Abstract

Abstract. To understand human papillomavirus (HPV) and expression of osteopontin (OPN) in cervical diseased tissues, HPV infection was detected in paraffin-embedded specimens of cervical lesions from 90 patients with cervical cancer. Three polymerase chain reaction (PCR) techniques were used to determine the detectable rate of HPV infection. Expression of HPV OPN protein was detected using immunohistochemical methods. When a pairwise comparison was made among the three PCR methods, χ 2 analysis indicated P>0.05 for detection through the methods of MY09/11 and GP5 + /6 + , and P>0.05 through the methods of MY09/11 and Nested-PCR. This indicated that there was no statistically significant difference in the HPV infection detection sensitivity of these methods. However, χ 2 comparison of the methods of GP5 + /6 + and Nested-PCR indicated P<0.05, which demonstrated that there was a statistically significant difference. The rate of positive HPV DNA measured with Nested-PCR was significantly higher than that measured using the GP5 + /6 + PCR method. The HPV OPN protein is expressed in cervical cancer, and the HPV OPN polypeptide antibody has broad spectrum reaction capacity and significant multivalence for HPV infection. Immunohistochemical detection was performed on tissue specimens using the purified rabbit HPV OPN polypeptide antibody. Sixty-one cases exhibited a positive result and 29 a negative result. The total rate of positive detection was 67.78%. HPV OPN may therefore serve as a candidate target for tumor treatment, including targeted therapies and vaccine development.

MicroRNA‐224 inhibition prevents progression of cervical carcinoma by targeting PTX3

“…23,24 Further, the 5-year overall rate of advanced-stage patient survival has remained below 40%. 23,24 Further, the 5-year overall rate of advanced-stage patient survival has remained below 40%.…”

“…Cervical carcinoma is a malignant solid tumor, and it is also one of the main causes of both morbidity and mortality in women, with China having approximately one-third of the incidence and mortality in the world based on statistical data. 23,24 Further, the 5-year overall rate of advanced-stage patient survival has remained below 40%. 25 Thus, it is paramount to explore more effective prognostic evaluations as well as to identify improved therapeutic strategies.…”

Biological and Clinicopathological Characteristics of OPN in Cervical Cancers

“…In previous reports, OPN was found to act as an oncogene during tumorigenesis of CC ( Cho et al, 2008 ; Bao et al, 2015 ; Xu et al, 2019 ). High expression of OPN was correlated with a higher FIGO stage, larger tumor size, lymph node metastasis, HPV infection, cisplatin resistance and shorter survival ( Bao et al, 2015 ; Huang et al, 2015 ; Xu et al, 2019 ).…”

“…In previous reports, OPN was found to act as an oncogene during tumorigenesis of CC ( Cho et al, 2008 ; Bao et al, 2015 ; Xu et al, 2019 ). High expression of OPN was correlated with a higher FIGO stage, larger tumor size, lymph node metastasis, HPV infection, cisplatin resistance and shorter survival ( Bao et al, 2015 ; Huang et al, 2015 ; Xu et al, 2019 ). Nonetheless, the immunological and metabolic features of OPN in CC have not been sufficiently investigated, and clinical characteristic analysis using gene datasets from multiple databases has never been performed.…”

The Roles of Matricellular Proteins in Oncogenic Virus-Induced Cancers and Their Potential Utilities as Therapeutic Targets

“…The experimental expression of the E6 and E7 proteins of HPV-16 increased the expression of VEGF/interleukin-8 and decreased that of TSP-1 in keratinocytes ( Table 1 ) [ 96 , 97 , 98 ], suggesting that those HPV proteins are involved in angiogenesis. Bao et al reported that OPN was highly detected in HPV-positive patients with cervical cancer, suggesting that OPN could be involved in the HPV-associated progression of cervical cancer [ 99 ]. However, that study did not describe any relationship between the HPV proteins and the enhanced OPN expression in HPV-positive lesions.…”