MicroRNA‐224 inhibition prevents progression of cervical carcinoma by targeting PTX3

Abstract

Cervical carcinoma is known as one of the most lethal and common conditions in women worldwide. Increasing evidence shows that microRNAs (miRs) may be involved in the pathogenesis of cervical carcinoma. This study investigates the correlation between expression of miR-224 in peripheral blood mononuclear cells and both diagnosis and prognosis of cervical carcinoma to clarify the effect miR-224 has on the biological behaviors of the subjected cervical carcinoma cells. Initially, 132 patients diagnosed with cervical carcinoma and 120 healthy subjects were recruited. Peripheral blood expression of miR-224 and PTX3 was detected. A telephone follow-up was performed every 3 months after treatment. The diagnostic value of miR-224 in cervical carcinoma was analyzed using the Receiver Operating Characteristic curve. The effects of both miR-224 and PTX3 on cell proliferation, migration, and invasion were evaluated with an intervention of miR-224 ectopic expression or depletion and PTX3 silencing. The bioinformatics prediction website and dual-luciferase reporter assay revealed PTX3 to be a target gene for miR-224. Moreover, miR-224 was detected as over-expressed, but PTX3 was under-expressed in cervical carcinoma. Additionally, as a diagnostic biomarker, a high miR-224 expression was closely linked with the progression of cervical carcinoma. Both miR-224 overexpression and PTX3 silencing promoted cell proliferation, migration, and invasion, whereas, the aforementioned properties were depressed when miR-224 was inhibited. Altogether, the miR-224 overexpression may be a biological indicator in predicting the progression of cervical carcinoma. Thus, miR-224 inhibition may significantly prevent cervical carcinoma progression by targeting the PTX3 gene.

miR‑34c‑5p targets Notch1 and suppresses the metastasis and invasion of cervical cancer

“…miRNAs either degrade certain specific genes or inhibit their expression by binding to 3'-untranslated regions (3'-UTRs) of their mRNAs in a complimentary manner (5,6). miRNAs are widely involved in various biological processes including proliferation, tumor metastasis and drug tolerance (7,8). miR-34c-5p has been reported to function as a tumor suppressor in numerous cancer types (9).…”

miR-224 targets BTRC and promotes cell migration and invasion in colorectal cancer

“…The aberrant expression of hsa-miR-224-5p (miR-224) has been found to differ in distinct tumor tissues in comparison to adjacent normal tissues. It has been reported that miR-224 was upregulated in pancreatic cancer (Zhu et al 2018), renal cell carcinoma (Pan et al 2018), cervical 1 3 485 Page 2 of 10 carcinoma (Yu et al 2018), hepatocellular carcinoma (Hung et al 2018), and bladder cancer (Miao et al 2018), but low expression of miR-224 has been reported in prostate cancer (Gan et al 2018) and oral squamous cell carcinoma (Lu et al 2019). miR-224 seems to function differently in different cancer types.…”

MicroRNA‑432 is downregulated in cervical cancer and directly targets FN1 to inhibit cell proliferation and invasion

“…It has been demonstrated that a number of miRNAs are abnormally expressed during the occurrence and progression of cervical cancer (8). For example, miR-143 (9), miR-152 (10) and miR-506 (11) were downregulated in cervical cancer, whereas miR-21 (12), miR-224 (13) and miR-1297 (14) were expressed at high levels. Recent evidence has indicated that deregulated miRNAs may serve crucial roles in cervical cancer initiation and progression by regulating a wide range of biological functions (15)(16)(17).…”