Detection of Human Cytomegalovirus in patients with Colorectal Cancer by Nested-PCR

Abstract

Background: The association of colorectal cancer with human cytomegalovirus (HCMV) is a controversial issue in cancer research. This study aimed to identify the HCMV virus in colorectal cancer tissues and to investigate the association of HCMV with colorectal cancer. In this study, 50 cancer tissue samples and 50 samples without colon cancer were studied in order to identify the HCMV virus through nested-polymerase chain reaction. The cases of adenocarcinoma tissues were in a moderately differentiated stage, and 7 cases had well-differentiated of p<0.05. The HCMV virus could play a role in creating malignancy and the progress of cancer through the process of oncomodulation. 1455 DOI:http://dx.doi.org/10.7314/APJCP.2014.15.3 .1453 Detection of Human Cytomegalovirus in Patients with Colorectal Cancer by Nested-PCRadenocarcinoma tissues in the moderately differentiated stage and 7 cases in the well-differentiated stage were DiscussionIn vitro transform cells, and cause disruption along many cell to other studies on colorectal cancer, which point to a results, including; study limitations and methodology, kept in formalin for a long time will fail when compared to cell in the early stages of the infection, then transforms the in cancerous cells, cell cycle progress, stimulating intracellular signalling pathway, and increasing telomerase and more importantly, resistance against chemotherapy

Relative Frequency of Cytomegalovirus (CMV) in Tissue Samples of Women with Breast Cancer in Sanandaj, Iran.

“…After an initial denaturation phase at 95°C for three minutes, 45 cycles programmed were along these lines: denaturation phase at 95°C for 35 seconds, annealing phase at 55°C for 45 seconds, primer extension at 72°C for 40 seconds and final extension phase at 72°C for five minutes. PCR products were determined by visualization of amplicons on 2% agarose gels stained with Safe Stain (22).…”

“…The second-round PCR amplification was performed via 12.5μl of the Amplicon master mix, 0.5μl of forward and reverse primers and 5μl of the firstround PCR products as templates in 25μL reactions (22). The sequences of the inside primer pair were: 5'GCCCGCCGCGGCAGCACCTGGCT-3' and 5'GTAAACCACATCACCCGTGGA-3' (23).…”

“…In the last part of the amplification, 6 μL of the PCR products were analyzed on 2% agarose gel. It was anticipated that the resultant product is a 174-bp fragment (22).…”

Low Expression of Estrogen Receptor-α and Progesterone Receptor in Human Breast Cancer Tissues Is Associated With High-Grade Human Cytomegalovirus Protein Expression

“…Human cytomegalovirus (HCMV) has been detected in glioblastoma multiforme 14,15 , medulloblastoma 16 , neuroblastoma 17 , colon cancer 18,19 , and prostate cancer 20 , as well as in primary BC and sentinel lymph nodes and distant metastases from BC patients [21][22][23] . In general, increased viral activity is associated with worse prognosis.…”

Identification of human cytomegalovirus in tumour tissues of colorectal cancer and its association with the outcome of non-elderly patients

“…Some earlier studies reported negative detection of HCMV in the tumour tissue of CRC (Akintola-Ogunremi et al, 2005;Boguszakova et al, 1988;Brichacek et al, 1980). However, more and more recent data have indicated that HCMV preferentially infects the tumour tissue of CRC (Cai et al, 2016;Dimberg et al, 2013;Li et al, 2015;Salyakina & Tsinoremas, 2013;Sole et al, 2015;Tafvizi & Fard, 2014;Taher et al, 2014). There is also a growing body of evidence suggesting that HCMV plays an oncomodulatory role and affects the disease course of human cancers.…”