Detection of human cytokines in situ using antibody and probe based methods

Hoefakker, S.; Boersma, Wirn J.A.; Claassen, Eric

doi:10.1016/0022-1759(95)00122-q

Cited by 33 publications

(24 citation statements)

References 122 publications

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“…Primary mAbs directed to the following antigens were used at predefined concentrations: CD3 (DAKO), CD4 (DAKO), CD8 (Monosan, Canton, MA), CD69 (Biolegend, San Diego, CA), IL-15 (R&D Systems, Minneapolis, MN), grB (DAKO), HLA class II (DAKO), VZV ORF62 (Millipore, Billerica, MA), and the appropriate isotype controls. Stainings were made on acetone-fixed cryosections, or paraformaldehydefixed paraffin sections, in case of the combined analysis of grB and CD8, with the avidin-biotin complex system (DAKO) (49). HSV-1 LAT in situ hybridization was performed on cryosections by using a digoxigenin-conjugated LAT oligonucleotide probe, as described (7).…”

Section: Ifn-␥ Elispotmentioning

confidence: 99%

Selective retention of herpes simplex virus-specific T cells in latently infected human trigeminal ganglia

Verjans¹,

Hintzen

Dun

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

198

227

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Section: Ifn-␥ Elispotmentioning

confidence: 99%

Selective retention of herpes simplex virus-specific T cells in latently infected human trigeminal ganglia

Verjans¹,

Hintzen

Dun

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

198

227

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“…The methodology of immunocytochemical procedures used here has been reviewed in detail previously. 21 In brief, frozen sections of 6 m were cut and thaw-mounted on glass slides. Slides were kept overnight at RT in humidified atmosphere.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Marked elevation of the chemokine CCL18/PARC in Gaucher disease: a novel surrogate marker for assessing therapeutic intervention

Boot

Verhoek

Fost

et al. 2004

Blood

298

210

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“…In situ hybridization was performed as described by Hoefakker et al (1995). Briefly, the digoxigenin-labelled riboprobe (5 ng µl -1 ) was added to a hybridization mixture containing 20% dextran sulphate, 12.5 mM Tris-HCl (pH 8.0), 2.5 × Denhardts solution, 2.5 mM EDTA, 2.5 mM DTT, 0.01 mg ml -1 herring sperm DNA, 0.002 mg ml -1 tRNA and 50% deionized formamide.…”

Section: Rna: Rna In Situ Hybridizationmentioning

confidence: 99%

Breast tumour cell-induced down-regulation of type I collagen mRNA in fibroblasts

et al. 1999

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This study investigated the modulation of type I collagen gene expression in normal fibroblasts by breast tumour cells. Northern analysis of total RNA extracted from stages I, II and III breast tumour tissue revealed that collagen mRNA levels were elevated in stage I tumours compared to the adjacent normal breast tissues, whereas they were decreased in stages II and III breast tumours. This aberrant collagen gene expression was confirmed by non-radioactive RNA:RNA in situ hybridization analysis of 30 breast carcinomas which localized the production of type I collagen mRNA to the stromal fibroblasts within the vicinity of the tumour cells. In order to determine whether the tumour cells were directly responsible for this altered collagen production by the adjacent fibroblasts, breast tumour cell lines were co-cultured with normal fibroblasts for in vitro assessment of collagen and steady-state collagen RNA levels. Co-culture of tumour cells and normal fibroblasts in the same dish resulted in down-regulation of collagen mRNA and protein. Treatment of the fibroblasts with tumour-cell conditioned medium also resulted in decreased collagen protein levels but the mRNA levels, however, remained unaltered. These results suggested that the tumour cells either secrete a labile ‘factor’, or express a cell surface protein requiring direct contact with the fibroblasts, resulting in down-regulation of collagen gene expression. Modulation of the ECM is a common characteristic of invading tumour cells and usually involves increased production of collagenases by the tumour cells or stromal fibroblasts. This study showed that tumour cells were also able to modulate collagen mRNA production by stromal fibroblasts, which may facilitate tumour cell invasion and metastasis. © 1999 Cancer Research Campaign

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Detection of human cytokines in situ using antibody and probe based methods

Cited by 33 publications

References 122 publications

Selective retention of herpes simplex virus-specific T cells in latently infected human trigeminal ganglia

Selective retention of herpes simplex virus-specific T cells in latently infected human trigeminal ganglia

Marked elevation of the chemokine CCL18/PARC in Gaucher disease: a novel surrogate marker for assessing therapeutic intervention

Breast tumour cell-induced down-regulation of type I collagen mRNA in fibroblasts

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