“…Primary mAbs directed to the following antigens were used at predefined concentrations: CD3 (DAKO), CD4 (DAKO), CD8 (Monosan, Canton, MA), CD69 (Biolegend, San Diego, CA), IL-15 (R&D Systems, Minneapolis, MN), grB (DAKO), HLA class II (DAKO), VZV ORF62 (Millipore, Billerica, MA), and the appropriate isotype controls. Stainings were made on acetone-fixed cryosections, or paraformaldehydefixed paraffin sections, in case of the combined analysis of grB and CD8, with the avidin-biotin complex system (DAKO) (49). HSV-1 LAT in situ hybridization was performed on cryosections by using a digoxigenin-conjugated LAT oligonucleotide probe, as described (7).…”