Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction

Saito, Shigeru; Ando, Yumiko; Furuki, K.; Kakimoto, Kazuhiro; Tanigawa, Takuo; Moriyama, I.; Ichijo, Motohiko; Nakamura, Masataka; Ohtani, Kiyoshi; Sugamura, Kazuo

doi:10.1111/j.1349-7006.1989.tb01718.x

Cited by 35 publications

(13 citation statements)

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“…In general, HTLV-1-infected individuals have antibodies to structural proteins of the virus. However, it has been reported that some virus carriers and individuals with HTLV-1-associated diseases do not have detectable antibodies to HTLV-1 structural proteins [10][11][12][13][14] . Most virus carriers remain asymptomatic during their life, and the cofactors required for disease progression are largely unknown.…”

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confidence: 99%

Mycosis fungoides in European Russia: No Antibodies to Human T Cell Leukemia Virus Type I Structural Proteins, but Virus-Like Sequences in Blood and Saliva

Morozov

Syrtsev²,

Ellerbrok³

et al. 2005

Intervirology

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Objective: Mycosis fungoides (MF) is the most frequent form of cutaneous T cell lymphoma (CTCL). Human T cell leukemia virus type 1 (HTLV-1) involvement in MF progression is a matter of debate. The goal of the investigation was to search for HTLV-1 markers in a group of MF patients from a nonendemic area to HTLV-1. Materials and Methods: Fifty MF patients and 60 healthy donors from Moscow and the Moscow region were examined for HTLV-1 markers by Western blot, PCR, nested PCR, PCR/Southern hybridization, TaqMan real-time PCR and sequencing. Results: Plasma samples from MF patients were repeatedly negative for antibodies to HTLV-1 structural proteins. HTLV-1 tax-related sequences (corresponding to the second exon) were found in blood from 20 of 50 MF patients and in 3 of 5 saliva specimens. Three of 8 sequenced tax-like amplimers were identical and 5 of 8 contained 1–2 substitutions. tax transcripts and antibodies to p40^tax were detected in some ‘PCR-tax’-positive MF patients. Defective HTLV-1 genomes were demonstrated in 2 of 50 MF patients. Phylogenetic analysis of the defective genome 5′-LTR sequence revealed a relationship with HTLV-1a sequences from the transcontinental subgroup of HTLV-1. Conclusions: HTLV-1 tax-like sequences were revealed in blood and for the first time in saliva from MF patients living in an HTLV-1 nonendemic region. Expression of tax-like sequences was confirmed by both reverse transcription PCR and Western blot.

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confidence: 99%

Mycosis fungoides in European Russia: No Antibodies to Human T Cell Leukemia Virus Type I Structural Proteins, but Virus-Like Sequences in Blood and Saliva

Morozov

Syrtsev²,

Ellerbrok³

et al. 2005

Intervirology

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“…Therefore, based on these results, it is concluded that the overall incidence of vertical transmission is relatively low (33.3%, four infants/twelve HCV antibody seropositive mothers; 57%, four infants/seven HCV-RNA seropositive mothers). This incidence is the same as that of HTLV-1(human T-cell leukemia virus type-1; almost 40% in cases of breast feeding (Saito et al 1990)) but less than that of HBV (hepatitis B virus; 70% in HBs and HBe antigens positive women (Biswac et al 1989)). …”

Section: Resultsmentioning

confidence: 63%

The Incidence of Vertical Transmission of Hepatitis C Virus.

Uehara

Abe

Saito

et al. 1993

Tohoku J. Exp. Med.

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This study was undertaken to clarify the incidence of the vertical transmission of hepatitis C virus (HCV). During the third trimester, 2015 pregnant women were examined as to anti-HCV antibodies. Anti-HCV antibody seropositive women were examined for HCV-RNA in peripheral blood at labor and in breast milk. Their offspring were also examined for HCV-RNA in umbilical cord blood and peripheral blood one week after birth and during subsequent outpatient visits. The following results were obtained: (1) Twelve of the 2015 pregnant women (0.6%) were seropositive for anti-HCV antibodies; (2) Seven of the twelve women (58%) seropositive for anti-HCV antibodies were also seropositive for HCV-RNA; (3) Three newborns of the seven HCV-RNA seropositive women (43%) were found to have HCV-RNA in the cord blood; (4) In the three newborns HCV-RNA had disappeared from the peripheral blood within one month after birth; (5) Two of the seven HCV-RNA seropositive women (29%) had HCV-RNA positive breast milk; (6) The possibility of infection via breast milk was shown in one infant at ten months after birth. Based on these results, it is indicated that HCV vertical transmission is possible in more than half of the HCV-RNA seropositive mothers. However, because of the disappearance of HCV from the infants' peripheral blood, further following study is needed.hepatitis C virus (HCV); vertical transmission; intrauterine infection; breast feeding Hepatitis C virus (HCV) has been recently detected, and it has been revealed that in most cases, non-A, non-B (NANB) hepatitis is induced by HCV infection

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“…While in the majority of cases HTLV-I/II sequences are not detected by PCR, there are some cases in which it is possible to amplify by PCR sequences of different HTLV-I/II genes (Saito et al 1989, Delaporte et al 1991, Miyata et al 1995, since some of them become seropositive over time. The data reported suggests the existence of HTLV-I/II infected subjects who are serologically negative.…”

Section: Discussionmentioning

confidence: 99%

Usefulness of a Nested-polymerase chain reaction for molecular diagnosis of human T-cell lymphotropic virus type I/II

Gallego

Mangano

Gastaldello

et al. 2004

Mem. Inst. Oswaldo Cruz

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Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction

Cited by 35 publications

References 9 publications

Mycosis fungoides in European Russia: No Antibodies to Human T Cell Leukemia Virus Type I Structural Proteins, but Virus-Like Sequences in Blood and Saliva

Mycosis fungoides in European Russia: No Antibodies to Human T Cell Leukemia Virus Type I Structural Proteins, but Virus-Like Sequences in Blood and Saliva

The Incidence of Vertical Transmission of Hepatitis C Virus.

Usefulness of a Nested-polymerase chain reaction for molecular diagnosis of human T-cell lymphotropic virus type I/II

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