2004
DOI: 10.1590/s0074-02762004000400006
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Abstract: This study aimed at implementing a Nested-polymerase chain reaction (Nested-PCR) for the molecular diagnosis of human T-cell lymphotropic virus type I/II (HTLV-I and HTLV-II)

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Cited by 7 publications
(21 citation statements)
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References 24 publications
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“…For nested-PCR, DNA extraction from the buffy coat of the blood samples was performed using a QIAamp DNA Blood Mini Kit (QIAGEN, Netherlands) and the HTLV virus DNA was detected with an in-house nested-PCR following the protocols described elsewhere,25,26 using MT-2 cells infected with HTLV-1 as a positive control. Briefly, two sets of primers for the tax/rex gene were used: for the first PCR reaction the primers fw 5ʹ-CGGATACCCAGTCTACGTGT-3ʹ and rv 5ʹ-GAGCCGATAACGCGTCCATC-3ʹ and for the nested-PCR reaction primers fw 5ʹ-GTGTTTGGCGATTGTGTACA-3ʹ and rv 5ʹ-CCATCGATGGGGTCCCA-3ʹ.…”
Section: Methodsmentioning
confidence: 99%
“…For nested-PCR, DNA extraction from the buffy coat of the blood samples was performed using a QIAamp DNA Blood Mini Kit (QIAGEN, Netherlands) and the HTLV virus DNA was detected with an in-house nested-PCR following the protocols described elsewhere,25,26 using MT-2 cells infected with HTLV-1 as a positive control. Briefly, two sets of primers for the tax/rex gene were used: for the first PCR reaction the primers fw 5ʹ-CGGATACCCAGTCTACGTGT-3ʹ and rv 5ʹ-GAGCCGATAACGCGTCCATC-3ʹ and for the nested-PCR reaction primers fw 5ʹ-GTGTTTGGCGATTGTGTACA-3ʹ and rv 5ʹ-CCATCGATGGGGTCCCA-3ʹ.…”
Section: Methodsmentioning
confidence: 99%
“…In cases where Western blot proves inconclusive, molecular approaches should be used 8,13,14 which show, especially when PCR is used, a higher degree of specificity. 15 Real-time PCR allows the detection and quantification of target sequences by continuously measuring the fluctuations in fluorescence during reaction amplification. This is carried out in a closed system, thereby diminishing the risk of contamination and eliminating the gel phase of standard PCR.…”
Section: Discussionmentioning
confidence: 99%