Detection of HPV DNA in Cervical Specimens Collected in Cytologic Solution by Ligation-Dependent PCR

Yarkın, Fügen; Chauvin, Sara; Konomi, Nami; Wang, Wayne; Mo, Richard; Bauchman, Gail; Díaz, Ángela; Burstein, David; Szporn, Arnold H.; E, Hauptman; Zhang, David Y.

doi:10.1159/000326549

Cited by 8 publications

(12 citation statements)

References 30 publications

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“…Dü şük risk HPV tip le rin den HPV 6 ve 11 ise ge ni tal si ğil le rin %90'ın dan faz la sın da et kendir. 1,2 Ser vi kal kan ser, bü tün dün ya da, ka dın lar da gö ğüs kan se rin den son ra gö rü len ikin ci en yay gın kan ser dir. HPV enfeksiyonu cin sel yol la bu la şan en yay gın vi ral enfeksiyon lar dan bi ri olup ka dın la rın %50'den faz la sı ya şam la rı bo yun ca HPV enfeksiyonu ile kar şı la şır.…”

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The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

Altun¹,

Yarkın²,

Vardar³

et al. 2011

Turkiye Klinikleri J Med Sci

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Ö ÖZ ZE ET T A Am ma aç ç: : Hu man papillomavirus (HPV) ser vi kal kan ser ile iliş ki si gös te ril miş ma jör et yo lo jik ajan dır. Ser vi kal kan ser tüm dün ya da ka dın lar ara sın da en yay gın ikin ci kan ser dir. Bu ça lış ma da Çu-ku ro va Üni ver si te si Tıp Fa kül te si Has ta ne si ne baş vu ran ka dın lar da hu man papillomavirus enfeksiyonu nun pre va lan sı nın araş tı rıl ma sı amaç lan mış tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Top lam 460 ser vi kal si to lo ji ör ne ği 20-68 yaş ara sı ka dın lar dan top lan mış tır. HPV DNA tes pi ti için MY09/11 ve GP5+/6+ pri mer le ri ile ya pı lan kon sen sus PCR (poly me ra se cha in re ac ti on) tes ti kul la nıl mış tır. HPV DNA pozi tif ör nek ler HPVpU-1M/pU-2R ve HPVpU-31B/pU-2R pri mer le ri ile sı ra sıy la yük sek risk li (HR; high risk) ve dü şük risk li (LR; low risk) tip ler ola rak iden ti fi ye edil miş tir. Ay rı ca HPV DNA po zitif bu lu nan ör nek ler ser vi kal kan ser de en yay gın gö rü len HR HPV tip le ri olan HPV 16, 18, 31 ve 45 için tip spe si fik PCR ile ge no tip len di ril miş tir. B Bu ul l g gu u l la ar r: : Top lam 460 ör nek ten 24 (%5.2)'ü HPV DNA için po zi tif bu lun muş tur. HPV DNA po zi tif 24 ka dın dan 14'ün de (%3) tek ve ya mul tip le enfeksiyon şek lin de HR HPV ti pi ve 10'un da (%2.2) ise tek ba şı na LR HPV po zi tif li ği tes pit edil miş -tir. HPV DNA po zi tif 30 yaş ve üs tü olan 24 ka dın da %33.3 ora nı ile en sık gö rü len HR HPV ti pi HPV tip 16 olup bu nu sı ra sıy la HPV 45 (%20.8), HPV 18 (%4.2) ve HPV 31 (%4.2) iz le miş tir. S So o n nu uç ç: : Çu ku ro va Üni ver si te si Tıp Fa kül te si Has ta ne sine baş vu ran ka dın lar da ge ni tal HPV enfeksiyon pre va lan sı %5.2 bu lun muş tur. HPV enfeksiyonu nun epi de mi yo lo jik araş tır ma la rı nın ser vi kal kan ser ta ra ma stra te ji le ri nin ge liş ti ril me si ve HPV aşı la rı nın uy gun lu ğu nun gös te ril me si için ge lecek te de sür dü rül me si öne ril mek te dir.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : İnsan papillomavirusü 16; in san papillomavirusü 18; po li me raz zin cir re ak si yo nu; ute rin ser vi kal tü mör ler; pre va lans A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Hu man pa pil lo ma virüs (HPV) is the ma jor eti o lo gic agent for cer vi cal cancer. Cer vi cal can cer is the se cond most pre va lent can cer among wo men in the world. In this study, we ai med to in ves ti ga te the pre va len ce of HPV in fec ti on among wo men ad mit ted to Hos pi tal of Çu -ku ro va Uni ver sity Fa culty of Me di ci ne. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : A to tal num ber of 460 cer vi cal smears we re ob ta i ned from wo men aged bet we en 20-68 ye ars. Con sen sus poly me ra se cha in re ac ti on (PCR) as say using MY09/11 and GP5 + /6 + pri mers we re used to de tect HPV DNA. HPV DNA po si ti ve samp les we re furt her stra ti fi ed as high risk (HR) and low risk (LR) by using HPVpU-1M/pU-2R and HPVpU-31B/pU-2R pri mers, res pec ti vely. HPV DNA po ...

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The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

Altun¹,

Yarkın²,

Vardar³

et al. 2011

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

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“…7,8 Each type of HPV leads to clinical problems and the genital types are classified as low or high risk according to their oncogenic potential. Human papilloma virus 6 and 11 are commonly found in genital warts, whereas HPV 16, 18, 31 and 45 are mostly involved in the development of invasive anogenital cancer.…”

Section: Discussionmentioning

confidence: 99%

Screening for and Genotyping of Human Papilloma Virus in Prostitutes

Keten¹,

Keten²,

Odabaşı³

et al. 2013

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T OOb bj je ec ct ti iv ve e: : The human papilloma virus (HPV) causes various infectious clinical pictures and anogenital cancers, and it is recognized as the primary etiological agent of cervical cancer worldwide. In this study, we aimed to demonstrate the presence of HPV in prostitutes and to identify its genotypes in cervical specimens. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : This study included 137 commercial sex workers from Ankara brothels who were referred to the Ankara Municipality Hospital, Department of Venereal Diseases from September 3-7, 2007 for routine examinations and who volunteered to participate. After obtaining informed consent from the participants, one-on-one interviews were conducted to obtain sociodemographic data. Cervical specimens were collected from each subject in order to investigate HPV genotypes by DNA extraction. R Re es su ul lt ts s: : The tests for HPV DNA were positive in 53 specimens following the extraction of DNA from swab specimens collected from the prostitutes. Seven cases had genotypes associated with the low-risk cancer group, one had genotypes affiliated with the undetermined risk group, and 38 had genotypes related to the high-risk group. In addition, genotypes associated with more than one risk group were detected in seven other study participants. Four subjects had genotypes related to both the undetermined indefinite and high-risk groups, two had genotypes connected with the low and high-risk groups, and one had genotypes from all three risk groups. Eighty-four participants tested negative for HPV DNA. C Co on nc cl lu us si io on n: : Women working in brothels are at risk for venereal diseases. This should be considered to protect both their own health and public health. It is noteworthy that the genotypes of HPV identified in the prostitutes were predominately of the high-risk cancerous type. Therefore, information regarding HPV should be distributed to women working in the sex industry, and preventive measures should be implemented to protect them from acquiring HPV. K Ke ey y W Wo or rd ds s: : Sexually transmitted diseases; community health planning Ö ÖZ ZE ET T A Am ma aç ç: : İnsan papillomavirüsleri [Human Papilloma Virüsü (HPV)] çesitli enfeksiyöz tablolara ve anogenital kanserlere neden olmaktadır. HPV bütün dünyada servikal kanserin primer etiyolojik etkeni olarak kabul edilmektedir. Bu çalışmadaki amaç, genelev kadınlarında, servikal örnekler kullanılarak, HPV varlığını ve genotiplerini ortaya koymaktır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : 3-7 Eylül 2007 tarihleri arasında Ankara Belediye Hastanesi Zührevi Hastalıklar Bölümü'ne rutin muayeneleri yapılmak üzere başvuran Ankara Genelevleri'nde çalışan 137 genelev kadını çalışmaya gönüllü olarak katılmıştır. Aydınlatılmış onamları alındıktan sonra çal-ışma grubu ile sosyodemografik veriler ile ilgili bilgi edinmek amacıyla birebir görüşme yapılmıştır. Olgulardan servikal sürüntü örnekleri alınarak bu örneklerde 21 tür HPV DNA'sının genotipi ar...

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“…A total of 22,417 women were described, with four articles [38,41,48,54] contributing fewer than 100 participants and 10 articles [14,21,25,26,31,37,39,42,52] (Luu et al, submitted), more than 500 participants. The article by Schiffman et al [42] had the largest sample size, with more than 3400 participants.…”

Section: Characteristics Of Included Articlesmentioning

confidence: 99%

“…Over half of the articles (k = 16) [14,21,25,31,35,36,39,43,[45][46][47][48][49][50][51]54] reported results from Europe, with the remainder from Asia-Pacific (k = 5) [26,44,52,56,57], North America (k = 6) (Luu et al, submitted) [37,38,42,53,55], and South America (k = 2) [40,41]. Just under half (k = 14) [25, 38-41, 44-46, 49-53, 57] reported studies were conducted in screening settings; 10 were in a follow-up/diagnostic setting [21,26,31,35,42,47,48,[54][55][56] and the remainder (k = 5) (Luu et al, submitted) [14,36,37,43] were in both screening and follow-up/diagnostic settings.…”

Section: Characteristics Of Included Articlesmentioning

confidence: 99%

Comparison of the accuracy of Hybrid Capture II and polymerase chain reaction in detecting clinically important cervical dysplasia: a systematic review and meta‐analysis

et al. 2013

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The effectiveness of screening programs for cervical cancer has benefited from the inclusion of Human papillomavirus (HPV) DNA assays; which assay to choose, however, is not clear based on previous reviews. Our review addressed test accuracy of Hybrid Capture II (HCII) and polymerase chain reaction (PCR) assays based on studies with stronger designs and with more clinically relevant outcomes. We searched OvidMedline, PubMed, and the Cochrane Library for English language studies comparing both tests, published 1985–2012, with cervical dysplasia defined by the Bethesda classification. Meta-analysis provided pooled sensitivity, specificity, and 95% confidence intervals (CIs); meta-regression identified sources of heterogeneity. From 29 reports, we found that the pooled sensitivity and specificity to detect high-grade squamous intraepithelial lesion (HSIL) was higher for HCII than PCR (0.89 [CI: 0.89–0.90] and 0.85 [CI: 0.84–0.86] vs. 0.73 [CI: 0.73–0.74] and 0.62 [CI: 0.62–0.64]). Both assays had higher accuracy to detect cervical dysplasia in Europe than in Asia-Pacific or North America (diagnostic odd ratio – dOR = 4.08 [CI: 1.39–11.91] and 4.56 [CI: 1.86–11.17] for HCII vs. 2.66 [CI: 1.16–6.53] and 3.78 [CI: 1.50–9.51] for PCR) and accuracy to detect HSIL than atypical squamous cells of undetermined significance (ASCUS)/ low-grade squamous intraepithelial lesion (LSIL) (HCII-dOR = 9.04 [CI: 4.12–19.86] and PCR-dOR = 5.60 [CI: 2.87–10.94]). For HCII, using histology as a gold standard results in higher accuracy than using cytology (dOR = 2.87 [CI: 1.31–6.29]). Based on higher test accuracy, our results support the use of HCII in cervical cancer screening programs. The role of HPV type distribution should be explored to determine the worldwide comparability of HPV test accuracy.

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Detection of HPV DNA in Cervical Specimens Collected in Cytologic Solution by Ligation-Dependent PCR

Cited by 8 publications

References 30 publications

The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

The Prevalence of Human Papilloma Virus Infection Among Women who Admitted to Çukurova University Faculty of Medicine Hospital

Screening for and Genotyping of Human Papilloma Virus in Prostitutes

Comparison of the accuracy of Hybrid Capture II and polymerase chain reaction in detecting clinically important cervical dysplasia: a systematic review and meta‐analysis

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