Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor

Eberly, Jed O.; Mayo, Michael L.; Carr, Matthew R.; Crocker, Fiona H.; Indest, Karl J.

doi:10.2323/jgam.2018.08.001

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…We previously described detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) with Escherichia coli ( E. coli ) containing a novel RNA riboswitch [ 18 ] developed for an aqueous environment by means of expression of the fluorescent DsRed protein [ 19 ]. The expression system consisted of an aptamer domain, linker sequence, and the DsRed Express2 gene.…”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence expression data was modeled (see below), and this model was then used to characterize the RDX detection efficacy of the riboswitch, and to provide insight into riboswitch characteristics and assay design constraints for efficient RDX detection. We generated a standard curve using purified DsRed protein (BioVision, Milpitas, CA), and measured fluorescence of the DsRed protein using a BioTek Synergy HT plate reader (BioTek Instruments, Inc., Winooski, VT) as previously described [ 18 ], with an excitation wavelength of 530 nm, an emission wavelength of 590 nm, and a PMT sensitivity rating of 35. These data establish a quantitative relationship between protein concentration and fluorescence ( S1 Fig ).…”

Section: Methodsmentioning

confidence: 99%

“…We previously described detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) with Escherichia coli (E. coli) containing a novel RNA riboswitch [18] developed for an aqueous PLOS ONE recommendations are those of the authors and are not necessarily endorsed by the U.S. Army. environment by means of expression of the fluorescent DsRed protein [19].…”

Section: Dsred Expression Dataset From Bacterial Culturementioning

confidence: 99%

“…Time series data for the riboswitch system generated from aptamer sequence 11, as described in Eberly et al[18]. Data represents both a control response (black circles) in addition to measurements from a 45 μM exposure of RDX (blue circles).…”

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confidence: 99%

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Modeling a synthetic aptamer-based riboswitch biosensor sensitive to low hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) concentrations

et al. 2020

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RNA aptamers are relatively short nucleic acid sequences that bind targets with high affinity, and when combined with a riboswitch that initiates translation of a fluorescent reporter protein, can be used as a biosensor for chemical detection in various types of media. These processes span target binding at the molecular scale to fluorescence detection at the macroscale, which involves a number of intermediate rate-limiting physical (e.g., molecular conformation change) and biochemical changes (e.g., reaction velocity), which together complicate assay design. Here we describe a mathematical model developed to aid environmental detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) using the DsRed fluorescent reporter protein, but is general enough to potentially predict fluorescence from a broad range of water-soluble chemicals given the values of just a few kinetic rate constants as input. If we expose a riboswitch test population of Escherichia coli bacteria to a chemical dissolved in media, then the model predicts an empirically distinct, power-law relationship between the exposure concentration and the elapsed time of exposure. This relationship can be used to deduce an exposure time that meets or exceeds the optical threshold of a fluorescence detection device and inform new biosensor designs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…We previously described detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) with Escherichia coli (E. coli) containing a novel RNA riboswitch [18] developed for an aqueous PLOS ONE recommendations are those of the authors and are not necessarily endorsed by the U.S. Army. environment by means of expression of the fluorescent DsRed protein [19].…”

Section: Dsred Expression Dataset From Bacterial Culturementioning

confidence: 99%

mentioning

confidence: 99%

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Modeling a synthetic aptamer-based riboswitch biosensor sensitive to low hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) concentrations

et al. 2020

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“…In past decades, many high-energy-density molecules have been designed in theory and synthesized in the laboratory, i.e. hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), 1,3,5,7-tetranitro-1,3,5,7tetraazacyclooctane (HMX), hexanitrohexaazaisowurtzitane (CL- 20), and so on [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Theoretical Study on Nitroimine Derivatives of Azetidine as High-Energy-Density Compounds

Li¹,

Li²

2020

Cent. Eur. J. Energ. Mater.

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A bacterial bioreporter for the detection of 1,3,5-trinitro-1,3,5-triazinane (RDX)

et al. 2021

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Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor

Cited by 7 publications

References 31 publications

Modeling a synthetic aptamer-based riboswitch biosensor sensitive to low hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) concentrations

Modeling a synthetic aptamer-based riboswitch biosensor sensitive to low hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) concentrations

Theoretical Study on Nitroimine Derivatives of Azetidine as High-Energy-Density Compounds

A bacterial bioreporter for the detection of 1,3,5-trinitro-1,3,5-triazinane (RDX)

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