Detection of herpesvirus genomes by polymerase chain reaction in cerebrospinal fluid and clinical findings

Minjolle, S.; Arvieux, Cédric; Gautier, Antoine; Jusselin, I.; Thomas, R.; Michelet, Christian; Colimon, R.

doi:10.1016/s1386-6532(02)00035-5

Cited by 36 publications

(32 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the first part of this study, herpesvirus DNA was found in 6.2% of CSF samples sent for herpesvirus PCR, which complies with previously reported detection rates between 2.1% and 13.9% [3][4][5]. The low rate may be due to low prediagnostic selection and generous indication for diagnostics as well as to the fact that the causative agent is not always present in detectable amounts [6].…”

Section: Discussionsupporting

confidence: 90%

“…EBV-DNA was detected in six patients with typical clinical findings and in six patients without symptoms compatible with an infection (3% of all patients tested). Other authors have previously investigated individuals with herpesvirus-DNA in CSF: none of 41 HSV-positive and only one of 18 EBV-positive patients had atypical clinical findings [3,4].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Herpesvirus DNA in Cerebrospinal Fluid and Correlation with Clinical Symptoms

et al. 2008

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Detection of Herpesvirus DNA in Cerebrospinal Fluid and Correlation with Clinical Symptoms

et al. 2008

View full text Add to dashboard Cite

show abstract

“…the use of antibiotics or the benefits of hospitalization 31 . Herpesviruses analysis by PCR has been found to present high sensitivity and sensibility and has definitely revolutionized virological diagnostic 28,[31][32][33] . Prior to PCR, the viral diagnosis of CNS infection was based on cellular culture of the CSF, brain biopsy or the demonstration of intrathecal antibody response, so that, in many instances the positive diagnostic was retrospective 34 .…”

Section: Discussionmentioning

confidence: 99%

Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil

Vidal

Almeida²,

Messias-Reason³

et al. 2011

Arq. Neuro-Psiquiatr.

View full text Add to dashboard Cite

Viral meningitis is a common infectious disease of the central nervous system (CNS) that occurs worldwide. The aim of this study was to identify the etiologic agent of lymphomonocytary meningitis in Curitiba, PR, Brazil. During the period of July 2005 to December 2006, 460 cerebrospinal fluid (CSF) samples with lymphomonocytary meningitis were analyzed by PCR methodologies. Fifty nine (12.8%) samples were positive. Enteroviruses was present in 49 (83%) samples and herpes virus family in 10 (17%), of these 6 (10%) herpes simplex virus, 1 (2%) Epstein Barr virus, 2 (3%) human herpes virus type 6 and 1 (2%) mixed infection of enterovirus and Epstein Barr virus. As conclusion enterovirus was the most frequent virus, with circulation during summer and was observed with higher frequency between 4 to 17 years of age. PCR methodology is an important method for rapid detection of RNA enterovirus and DNA herpesvirus in CSF.

show abstract

“…Saliva, blood, cerebrospinal fluid, tissue and skin lesion samples have all been used for HHV diagnosis by PCR [21][22][23][24][25][26] . Since HHVs have different susceptibilities to antiviral drugs, rapid and precise diagnosis of the HHVs involved in a specific pathology is very important.…”

Section: Ethicalmentioning

confidence: 99%

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Carvalho

Silvestre

Maciel

et al. 2010

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Human herpesviruses are frequently associated with orofacial diseases in humans (HSV-1, EBV, CMV and HHV-8), some can also cause systemic disease . The transmission of these viruses occurs by contact with infected secretions, especially saliva. Human immunodeficiency virus infection is associated with an increased risk of HHVs and related diseases. Methods: This work aimed to detect HSV-1, EBV, CMV and HHV-8 DNA in saliva of HIV-infected patients from Teresina, northeast Brazil, by PCR and compare these findings with age and sex matched HIV-seronegative individuals. Results: No difference in prevalence was verified between HHV detection in the saliva of HIV-seropositive individuals and controls. The individual frequencies of these viruses in these two populations were different. HIV seropositivity correlated positively with the presence of CMV (OR: 18.2, p= 0.00032) and EBV (OR: 3.44, p= 0.0081). No association between CD4 counts and the prevalence of HHVs in the saliva was observed; however, a strong association was determined between seropositivity and the presence of multiple HHV DNAs in saliva (OR: 4.83, p = 0.0028). Conclusions: These findings suggest the asymptomatic salivary shedding of HHVs is a common event between HIV-seropositive and seronegative individuals from Teresina, Piauí, Brazil, and, especially for HIV-seropositive patients, saliva is a risk factor for the acquisition/transmission of multiple HHVs. Key-words:Human herpesvirus. Saliva. PCR. HIV seropositive. RESUMOIntrodução: Alguns herpesvírus humanos são frequentemente associados a doenças orofaciais em humanos. A transmissão destes vírus ocorre através do contato com secreções contaminadas, especialmente a saliva. A infecção pelo vírus da imunodeficiência humana é considerada um fator de risco para a aquisição de HHVs e doenças correlatas. Métodos: Este trabalho teve como objetivo detectar por PCR o DNA de HSV-1, EBV, CMV e HHV-8 na saliva de pacientes infectados com HIV em Teresina, nordeste do Brasil, e comparar os dados obtidos com o grupo controle (indivíduos HIV negativos). Resultados: Não há diferença na prevalência de detecção de HHVs na saliva de indivíduos HIV soropositivos e soronegativos. No entanto, as frequências individuais de detecção dos diferentes HHVs são diferentes entre estas duas populações. A soropositividade para HIV apresentou correlação positiva com a presença de CMV (OR: 18,2, p = 0,00032) e EBV (OR: 3,44, p = 0,0081). Não foi verificada nenhuma associação entre a contagem de CD4 e prevalência de HHVs na saliva, no entanto existe uma forte associação entre a soropositividade e a detecção do DNA de vários HHVs na saliva (OR: 4,83, p = 0,0028). Conclusões: Estes resultados sugerem que a transmissão salivar de HHVs é um evento comum entre os indivíduos HIV soropositivos e soronegativos de Teresina, Piauí, Brasil, e, especialmente para os pacientes soropositivos, a saliva é um fator de risco para a aquisição/transmissão de múltiplos HHVs.Palavras-chaves: Herpesvirus humanos. Saliva. PCR. HIV sorop...

show abstract

Detection of herpesvirus genomes by polymerase chain reaction in cerebrospinal fluid and clinical findings

Cited by 36 publications

References 24 publications

Detection of Herpesvirus DNA in Cerebrospinal Fluid and Correlation with Clinical Symptoms

Detection of Herpesvirus DNA in Cerebrospinal Fluid and Correlation with Clinical Symptoms

Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Contact Info

Product

Resources

About