Detection of hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR)

Manjanaik, B; Umesha, Kanasinakatte R.; Karunasagar, Indrani; Karunasagar, Iddya

doi:10.3354/dao063255

Cited by 35 publications

(29 citation statements)

References 15 publications

(15 reference statements)

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“…HPV is highly prevalent in some areas, it has been found in nearly 100% of the populations of wild P. monodon in Africa and also in the wild stock of P. merguiensis in New Caledonia (Tang et al 2008). In India, the prevalence of HPV was found in 46% of the wild populations (Manjanaik et al 2005). For farmed P. monodon, 31 to 62% of shrimp were found to be HPV-positive in India and Thailand (Umesha et al 2003, Flegel et al 2004.…”

Section: Introductionmentioning

confidence: 99%

Duplex real-time PCR for detection and quantification of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon

Tang¹,

Lightner²

2011

Dis. Aquat. Org.

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We describe a duplex real-time PCR assay using TaqMan probes for the simultaneous detection of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV). Both MBV and HPV are shrimp enteric viruses that infect intestinal and hepatopancreatic epithelial cells. Both viruses can cause significant mortalities and depressed growth in infected larval, postlarval, and early juvenile stages of shrimp, and thus present a risk to commercial aquaculture. In this duplex assay, we combined 2 single real-time PCRs, amplifying MBV and HPV, in a one-tube PCR reaction. The 2 viruses were distinguished by specific fluorescent labels at the 5' end of TaqMan probes: the MBV probe was labeled with dichlorodimethoxyfluorescein (JOE), and the HPV probe was labeled with 6-carboxyfluorescein (FAM). The duplex real-time PCR assay was performed in a multi-channel real-time PCR detection system, and MBV and HPV amplification signals were separately detected by the JOE and FAM channels. This duplex assay was validated to be specific to the target viruses and found to have a detection limit of single copies for each virus. The dynamic range was found to be from 1 to 1 × 10 8 copies per reaction. This assay was further applied to quantify MBV and HPV in samples of infected Penaeus monodon collected from Malaysia, Indonesia, and Thailand. The specificity and sensitivity of this duplex real-time PCR assay offer a valuable tool for routine diagnosis and quantification of MBV and HPV from both wild and farmed shrimp stocks. KEY WORDS: Duplex real-time PCR · Monodon baculovirus · Hepatopancreatic parvovirus · Penaeus monodonResale or republication not permitted without written consent of the publisher

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Section: Introductionmentioning

confidence: 99%

Duplex real-time PCR for detection and quantification of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon

Tang¹,

Lightner²

2011

Dis. Aquat. Org.

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“…The amplification was carried out at 55°C annealing temperature, using the cycling protocol as mentioned by Tang et al (2000). Detection of HPV was done by nested PCR (Manjanaik et al, 2005). The primers H441F (5`-GCATTACAAGA GCCAAGCAG-3`) and H441R (5`-ACACTCAGC CTCTACCTTGT-3`) were used for first step PCR (Phromjai et al, 2002;Manjanaik et al, 2005;OIE, 2007).…”

mentioning

confidence: 99%

“…First step PCR was done following the protocol as optimised by Phromjai et al (2002). The cycling condition as per Manjanaik et al (2005) was used for second step PCR. The expected sizes of the amplified products were 441 and 265 bp for first and second step PCR, respectively.…”

mentioning

confidence: 99%

“…The primers H441F (5`-GCATTACAAGA GCCAAGCAG-3`) and H441R (5`-ACACTCAGC CTCTACCTTGT-3`) were used for first step PCR (Phromjai et al, 2002;Manjanaik et al, 2005;OIE, 2007). In second step PCR, 2 µl of product of first step PCR was amplified using the forward primer 5`-ATAGAACGCATAGAAAACGCT-3` and reverse primer 5`-GGTGGCGCTGGAATGAATC GCTG-3` as per Manjanaik et al (2005). First step PCR was done following the protocol as optimised by Phromjai et al (2002).…”

mentioning

confidence: 99%

“…For preparation of template DNA for detection of hepatopancreatic parvovirus (HPV), 20 mg of crab tomalley was used. Isolation of DNA template was carried out as per the protocol of Manjanaik et al (2005).…”

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confidence: 99%

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Occurrence of pathogenic shrimp viruses in selected wild crab species of Sunderban, India

2016

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Diseases in brackishwater aquaculture systems are the major hindrance to the growth of shrimp industry in India, with white spot viral disease being the most devastating one causing crop failure leading to heavy economic losses. In this study, wild crab species viz., Uca spp. and Sesarma spp., which are frequent dwellers surrounding the shrimp culture ponds, were screened for the presence of white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV) and hepatopancreatic parvovirus (HPV). Out of total 66 samples screened, presence of WSSV and IHHNV was detected in 18.2 and 1.5% of the samples, respectively. Results of the present study indicate that wild crab species found in and around shrimp ponds could act as carriers of shrimp pathogenic viruses and their effective control is essential for prevention of shrimp viral diseases.

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Nanotechnologies in the Health Management of Aquatic Animal Diseases

Raj

Arivarasan²,

Hameed³

et al. 2023

Nanotechnology in the Life Sciences

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Detection of hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR)

Cited by 35 publications

References 15 publications

Duplex real-time PCR for detection and quantification of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon

Duplex real-time PCR for detection and quantification of monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon

Occurrence of pathogenic shrimp viruses in selected wild crab species of Sunderban, India

Nanotechnologies in the Health Management of Aquatic Animal Diseases

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