Detection of Hepatocellular carcinoma in clinical specimens using Dielectrophoresis based ElectroKinetic Platform

Abdelbaset, Reda; Abo-Elela, Marwan; Ghallab, Yehya H.; Abdelhamid, Hamdy; Ali, Sameh S.; Sayed, Marwa M.; Shawky, Sherif M.; Ismail, Yehea

doi:10.1016/j.sna.2020.112402

Cited by 5 publications

(6 citation statements)

References 27 publications

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“…Abdelbaset et al. measured the velocity of hepatocellular carcinoma, healthy liver, and rat muscle cells under the application of the twDEP force [138]. At 500 kHz, in comparison to rat muscle cells, hepatocellular carcinoma and the healthy liver experienced a very large velocity.…”

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

“…Hepatocellular carcinoma cells were obtained from patients who had the underlying liver cancer. The cell samples were suspended in PBS buffer, and a sample volume of 20 μL was used in the experiments [138]. Figure 4D shows the workflow of the assay used in the experiments [138].…”

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

“…The cell samples were suspended in PBS buffer, and a sample volume of 20 μL was used in the experiments [138]. Figure 4D shows the workflow of the assay used in the experiments [138].…”

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

“…The study was conducted by Abdelbaset et al. [138]. Reprinted with permission from Ref[138], copyright (2020) Elsevier.…”

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

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Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021

et al. 2021

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Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021Dielectrophoresis is a well-understood phenomenon that has been widely utilized in biomedical applications. Recent advancements in miniaturization have contributed to the development of dielectrophoretic-based devices for a wide variety of biomedical applications. In particular, the integration of dielectrophoresis with microfluidics, fluorescence, and electrical impedance has produced devices and techniques that are attractive for screening and diagnosing diseases. This review article summarizes the recent utility of dielectrophoresis in assays of biomarker detection. Common screening and diagnostic biomarkers, such as cellular, protein, and nucleic acid, are discussed. Finally, the potential use of recent developments in machine learning approaches toward improving biomarker detection performance is discussed. This review article will be useful for researchers interested in the recent utility of dielectrophoresis in the detection of biomarkers and for those developing new devices to address current gaps in dielectrophoretic biomarker detection.

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Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

“…The cell samples were suspended in PBS buffer, and a sample volume of 20 μL was used in the experiments [138]. Figure 4D shows the workflow of the assay used in the experiments [138].…”

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

“…The study was conducted by Abdelbaset et al. [138]. Reprinted with permission from Ref[138], copyright (2020) Elsevier.…”

Section: Utility Of Dielectrophoresis In Biomarker Detectionmentioning

confidence: 99%

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Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021

et al. 2021

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“…Microfluidic is characterized by large surface to volume ratio, specificity, predictable fluidic phenomena, low cost, small sample volume requirement, and ability to integrate multiple functions [4]. These intrinsic advantages have made it suitable for developing nanoparticle diagnostic platforms.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic based Microfluidic for nanoparticles “viruses” separation

Abdelhamid

Khalaf

Hussien

et al. 2021

2021 3rd Novel Intelligent and Leading Emerging Sciences Conference (NILES)

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The objective of this paper is to provide a microfluidic platform that may be useful to manipulate and characterize different submicron particles such as latex spheres as well as viruses (E.g. SARS-COV-2) through the concept of dielectrophoresis. The significance of recent research towards microfluidics-based diagnostic chips is apparent in health care, in our homes, and also very prominently in the fight against the COVID 19 pandemic: Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), early diagnosis and management are crucial for containing the outbreak. Here, we report a microfluidic device for separating mixture of different viruses. Using appropriate microfluidic channels, flow velocity, microelectrode arrays and voltage settings, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on the effective dielectric properties of fluid and particles, so that a heterogeneous mixture of particles can be separated to produce a more homogeneous population. In this paper the controlled separation of nanoparticles is demonstrated. With upper and lower electrode arrays, it is shown that different types of submicron latex beads can be spatially separated. The separation occurs because of differences in magnitude and direction of the dielectrophoretic force and drag force on different populations of particles.

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Dielectrophoresis: Measurement technologies and auxiliary sensing applications

Hu,

Ji,

Chen

et al. 2024

Electrophoresis

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Dielectrophoresis (DEP), which arises from the interaction between dielectric particles and an aqueous solution in a nonuniform electric field, contributes to the manipulation of nano and microparticles in many fields, including colloid physics, analytical chemistry, molecular biology, clinical medicine, and pharmaceutics. The measurement of the DEP force could provide a more complete solution for verifying current classical DEP theories. This review reports various imaging, fluidic, optical, and mechanical approaches for measuring the DEP forces at different amplitudes and frequencies. The integration of DEP technology into sensors enables fast response, high sensitivity, precise discrimination, and label‐free detection of proteins, bacteria, colloidal particles, and cells. Therefore, this review provides an in‐depth overview of DEP‐based fabrication and measurements. Depending on the measurement requirements, DEP manipulation can be classified into assistance and integration approaches to improve sensor performance. To this end, an overview is dedicated to developing the concept of trapping‐on‐sensing, improving its structure and performance, and realizing fully DEP‐assisted lab‐on‐a‐chip systems.

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Detection of Hepatocellular carcinoma in clinical specimens using Dielectrophoresis based ElectroKinetic Platform

Cited by 5 publications

References 27 publications

Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021

Recent advances in dielectrophoresis toward biomarker detection: A summary of studies published between 2014 and 2021

Dielectrophoretic based Microfluidic for nanoparticles “viruses” separation

Dielectrophoresis: Measurement technologies and auxiliary sensing applications

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