Detection of hepatitis G virus envelope protein E2 antibody in blood donors

Ramezani, Amitis; Gachkar, Latif; Eslamifar, Ali; Khoshbaten, Manouchehr; Jalilvand, Somayeh; Adibi, Ladan; Salimi, Vahid; Hamkar, Rasool

doi:10.1016/j.ijid.2007.04.010

Cited by 16 publications

(17 citation statements)

References 46 publications

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“…In Iranian volunteer blood donors the prevalence of GBV-C was around 1% [46]. A study of prevalence of GBV-C among northeastern Thai blood donors carrying HBsAg and anti-HCV revealed a higher frequency of GBV-C RNA (10% and 11%, respectively) in the co-infected when compared with the controls [47].…”

Section: Discussionmentioning

confidence: 99%

Frequency and genotypic distribution of GB virus C (GBV-C) among Colombian population with Hepatitis B (HBV) or Hepatitis C (HCV) infection

Alvarado-Mora

Botelho

Nishiya³

et al. 2011

Virol J

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BackgroundGB virus C (GBV-C) is an enveloped positive-sense ssRNA virus belonging to the Flaviviridae family. Studies on the genetic variability of the GBV-C reveals the existence of six genotypes: genotype 1 predominates in West Africa, genotype 2 in Europe and America, genotype 3 in Asia, genotype 4 in Southwest Asia, genotype 5 in South Africa and genotype 6 in Indonesia. The aim of this study was to determine the frequency and genotypic distribution of GBV-C in the Colombian population.MethodsTwo groups were analyzed: i) 408 Colombian blood donors infected with HCV (n = 250) and HBV (n = 158) from Bogotá and ii) 99 indigenous people with HBV infection from Leticia, Amazonas. A fragment of 344 bp from the 5' untranslated region (5' UTR) was amplified by nested RT PCR. Viral sequences were genotyped by phylogenetic analysis using reference sequences from each genotype obtained from GenBank (n = 160). Bayesian phylogenetic analyses were conducted using Markov chain Monte Carlo (MCMC) approach to obtain the MCC tree using BEAST v.1.5.3.ResultsAmong blood donors, from 158 HBsAg positive samples, eight 5.06% (n = 8) were positive for GBV-C and from 250 anti-HCV positive samples, 3.2%(n = 8) were positive for GBV-C. Also, 7.7% (n = 7) GBV-C positive samples were found among indigenous people from Leticia. A phylogenetic analysis revealed the presence of the following GBV-C genotypes among blood donors: 2a (41.6%), 1 (33.3%), 3 (16.6%) and 2b (8.3%). All genotype 1 sequences were found in co-infection with HBV and 4/5 sequences genotype 2a were found in co-infection with HCV. All sequences from indigenous people from Leticia were classified as genotype 3. The presence of GBV-C infection was not correlated with the sex (p = 0.43), age (p = 0.38) or origin (p = 0.17).ConclusionsIt was found a high frequency of GBV-C genotype 1 and 2 in blood donors. The presence of genotype 3 in indigenous population was previously reported from Santa Marta region in Colombia and in native people from Venezuela and Bolivia. This fact may be correlated to the ancient movements of Asian people to South America a long time ago.

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Section: Discussionmentioning

confidence: 99%

Frequency and genotypic distribution of GB virus C (GBV-C) among Colombian population with Hepatitis B (HBV) or Hepatitis C (HCV) infection

Alvarado-Mora

Botelho

Nishiya³

et al. 2011

Virol J

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“…In an Amerindian population from Venezuela a high prevalence of GBV-C genotype 3 was observed, ranging from 5% (9 out of 162) in the West to 25% (14 out of 56) in the South region of the country [45]. The prevalence of GBV-C was 9.7% among 545 blood donors in Sao Paulo, Brazil [47], 8.3% in 1,039 healthy individuals, also from São Paulo, Brazil [48] or 1% from 478 Iranian volunteer blood donors [49].…”

Section: Introductionmentioning

confidence: 94%

Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in Sao Paulo, Brazil

et al. 2011

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BackgroundThe results of previous studies elsewhere have indicated that GB virus C (GBV-C) infection is frequent in patients infected with the human immunodeficiency virus type 1 (HIV-1) due to similar transmission routes of both viruses. The aim of this study was to determine the prevalence, incidence density and genotypic characteristics of GBV-C in this population.Methodology/Principal FindingsThe study population included 233 patients from a cohort primarily comprised of homosexual men recently infected with HIV-1 in São Paulo, Brazil. The presence of GBV-C RNA was determined in plasma samples by reverse transcriptase-nested polymerase chain reaction and quantified by real-time PCR. GBV-C genotypes were determined by direct sequencing. HIV viral load, CD4+ T lymphocyte and CD8+ T lymphocyte count were also tested in all patients. The overall prevalence of GBV-C infection was 0.23 (95% CI: 0.18 to 0.29) in the study group. There was no significant difference between patients with and without GBV-C infection and Glycoprotein E2 antibody presence regarding age, sex, HIV-1 viral load, CD4+ and CD8+T cell counts and treatment with antiretroviral drugs. An inverse correlation was observed between GBV-C and HIV-1 loads at enrollment and after one year. Also, a positive but not significant correlation was observed between GBV-C load and CD4+ T lymphocyte. Phylogenetic analysis of the GBV-C isolates revealed the presence of genotype 1 and genotype 2, these sub classified into subtype 2a and 2b.Conclusion/SignificanceGBV-C infection is common in recently HIV -1 infected patients in Sao Paulo, Brazil and the predominant genotype is 2b. This study provides the first report of the GBV-C prevalence at the time of diagnosis of HIV-1 and the incidence density of GBV-C infection in one year.

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“…Anti-E2 antibody can be distinguished in the beginning of infection and also later, along with HGV RNA, suggesting a window period between clearance of RNA and reappearance of anti-E2 antibody (14). There is an enzyme-linked immunosorbent assay (ELISA) test for the detection of anti-E2 antibody and a reverse transcription polymerase chain reaction (RT-PCR) test for the detection of RNA to diagnose HGV infection (15,16). The aim of this study was to epidemiologically survey prevalence and genotype distribution of HGV in patients with hepatitis B and C in the southwest of Iran.…”

Section: Introductionmentioning

confidence: 99%

Hepatitis G virus (HGV), its prevalence and genotypes in patients with hepatitis B & C in Ahvaz, Southwest Iran

Javanmard¹,

Makvandi²,

Hajiani³

et al. 2013

Turk J Med Sci

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The crystal structure of (E)-3- phenylimino)-methyl]benzene-1,2-diol was determined using X-ray diffraction and the molecular structure was investigated with density functional theory (DFT). The X-ray study showed that the title compound has a strong intramolecular O-H. . . N hydrogen bond and 3-dimensional crystal networks are primarily determined by O-H. . . O intermolecular hydrogen bonds and weak van der Waals interactions. The strong O-H. . . N bond is evidence of the preference for the phenol-imine tautomeric form in the solid state. Optimized molecular geometry was calculated with DFT at the B3LYP/6-31G(d,p) level. The results from both experiment and theoretical calculations for the title compound are compared with each other in this study.

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Detection of hepatitis G virus envelope protein E2 antibody in blood donors

Cited by 16 publications

References 46 publications

Frequency and genotypic distribution of GB virus C (GBV-C) among Colombian population with Hepatitis B (HBV) or Hepatitis C (HCV) infection

Frequency and genotypic distribution of GB virus C (GBV-C) among Colombian population with Hepatitis B (HBV) or Hepatitis C (HCV) infection

Prevalence, Incidence Density, and Genotype Distribution of GB Virus C Infection in a Cohort of Recently HIV-1-Infected Subjects in Sao Paulo, Brazil

Hepatitis G virus (HGV), its prevalence and genotypes in patients with hepatitis B & C in Ahvaz, Southwest Iran

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