Detection of Helicobacter pylori vacA , cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients

El‐Shenawy, A. I.; Diab, Manal; Shemis, Mohamed; El-Ghannam, Maged; Salem, Dalia; Abdelnasser, Moustafa; Shahin, Mohamed; Abdel-Hady, Mahmoud; El-Sherbini, Effat; Saber, Mohamed

doi:10.1016/j.ejmhg.2017.04.003

Cited by 18 publications

(16 citation statements)

References 47 publications

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“…According to literature, vacA gene possesses variable sequences in s (s1 and s2) and m (m1 and m2) regions. It is notable that vacA s1m1 has the most expression rate, and therefore high vacuolating, but vacA s1m2 is a moderate vacuolating genotype, as well as vacA s2m2 is not toxic, and finally, vacA s2m1 genotype is rare and non-toxic [75][76][77][78]. Recently, two additional variable regions, i1/i2 and d1/d2, have recognized in m region, and also, each of s1 and m1 regions subdivided to different types such s1a, s1b, s1c, m1a, m1b and m1c [79,80].…”

Section: Discussionmentioning

confidence: 99%

Association of Helicobacter pylori vacA genotypes and peptic ulcer in Iranian population: a systematic review and meta-analysis

Keikha

Ali‐Hassanzadeh

2020

BMC Gastroenterol

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Background: Helicobacter pylori is accounted as the most etiologic agent for digestive disorders, in particular, the most important of them i.e. peptic ulcer and gastric cancer. In the recent years, association of vacA genotypes and gastrointestinal disorders has attracted a lot of attention. In present study, we assessed the correlation between vacA genotypes (s1, s2, m1, m2, s1m1, s1m2, s2m1 and s2m2) and development to peptic ulcer in Iranian population. Methods: In our study, first, 24 original articles containing of information of 3328 patients were evaluated. Statistical analysis was done by Comprehensive Meta-Analysis version 2.0 software (Biostat, Englewood, NJ, USA). In this regards, we used from fixed-effects model for analysis of data with low heterogeneity, while for analysis of data with high heterogeneity (I 2 statistic index > 25%, Cochrane Q statistic p value < 0.05), random-effects model was used.

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Section: Discussionmentioning

confidence: 99%

Association of Helicobacter pylori vacA genotypes and peptic ulcer in Iranian population: a systematic review and meta-analysis

Keikha

Ali‐Hassanzadeh

2020

BMC Gastroenterol

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“…In Egypt, several studies investigated the prevalence of cagA and they reported variable results [ 20 , 33 , 34 ]. Amer and her colleagues (2013) reported high prevalence of cagA gene (65%) [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, Said Essa and his colleagues (2008) reported that 62.2% of H. pylori infected patients were cagA positive [ 33 ]. On the other hand, El-Shenawy and his colleagues (2017) reported low prevalence of cagA gene (26.6%) [ 34 ]. This could be attributed to different sample sizes, different socioeconomic status, and living conditions of the studied patients.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

Abutaleb

Abdelattef

Abdel-Hady

et al. 2018

International Journal of Microbiology

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H. pylori infection causes peptic ulcer, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. It has several virulence factors such as cytotoxin-associated gene A(cagA) and the induced by contact with epithelium antigen (iceA). We aimed to explore the relationship between cagA and iceA of H. pylori and gastrointestinal diseases. One hundred and eighteen patients who attended Gastrointestinal Endoscopy Unit at Zagazig University Hospitals, Egypt, were included in this study. Two gastric biopsies were collected and evaluated by rapid urease test (RUT) and PCR. cagA and iceA genes were amplified by PCR. We found that 54 patients (45.76%) were positive by both RUT and PCR. cagA and iceA genes were present in 57.4% and 46.29% of the studied patients, respectively. cagA was the most prevalent gene in gastritis (33.3%) and peptic ulcer (68.7%). iceA1/iceA2 positive genes were the most prevalent in gastric cancer (75%). iceA1 gene was present in 38.7% of cagA positive cases, but iceA2 gene was present in 45.2% of cagA positive cases. iceA1/iceA2 positive genes were present in 29% of cagA positive cases. In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene.

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“…According to literature, vacA gene possesses variable sequences in s (s1 and s2) and m (m1 and m2) regions. It is notable that vacA s1m1 has the most expression rate, and therefore high vacuolating, but vacA s1m2 is a moderate vacuolating genotype, as well as vacA s2m2 is not toxic, and nally, vacA s2m1 genotype is rare and non-toxic (71)(72)(73)(74). Recently, two additional variable regions, i1/i2 and d1/d2, have recognized in m region, and also, each of s1 and m1 regions subdivided to different types such s1a, s1b, s1c, m1a, m1b and m1c (75)(76).…”

Section: Discussionmentioning

confidence: 99%

Association of Helicobacter pylori vacA genotypes and peptic ulcer in Iranian population: a systematic review and meta-analysis

Keikha

Ali‐Hassanzadeh

2020

Preprint

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Background Helicobacter pylori is accounted as the most etiologic agent for digestive disorders, in particular, the most important of them i.e. peptic ulcer and gastric cancer. In the recent years, association of vacA genotypes and gastrointestinal disorders has attracted a lot of attention. In present study, we assessed the correlation between vacA genotypes (s1, s2, m1, m2, s1m1, s1m2, s2m1 and s2m2) and development to peptic ulcer in Iranian population. Methods In our study, first, 23 original articles containing of information of 3328 patients were evaluated. Statistical analysis was done by Comprehensive Meta-Analysis version 2.0 software (Biostat, Englewood, NJ, USA). In this regards, we used from fixed-effects model for analysis of data with low heterogeneity, while for analysis of data with high heterogeneity (I2 statistic index > 25%, Cochrane Q statistic p value < 0.05), random-effects model was used. Results Abundance of each of s1, s2, m1, m2, s1m1, s1m2, s2m1, and s2m2 was estimated 36.24%, 28.32%, 42.90% 29.86%, 27.88%, 32.34%, 15.70%, and 25.94%, respectively. According to the results, the m1, s1, and s1m2 genotypes were among the most prevalent genotypes among the Iranian patients, whereas, s2m1 genotype had the lowest frequency. Conclusions Finally, we demonstrated that there is a significant relationship between infection of stomach with m1, s1m1, and s2m1 genotypes and development to peptic ulcer.

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Detection of Helicobacter pylori vacA , cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients

Cited by 18 publications

References 47 publications

Association of Helicobacter pylori vacA genotypes and peptic ulcer in Iranian population: a systematic review and meta-analysis

Association of Helicobacter pylori vacA genotypes and peptic ulcer in Iranian population: a systematic review and meta-analysis

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

Association of Helicobacter pylori vacA genotypes and peptic ulcer in Iranian population: a systematic review and meta-analysis

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