Detection of Helicobacter pylori in recurrent aphthous ulceration by nested PCR

Abstract

Recurrent aphthous ulceration (RAU) is the most common lesion of the oral mucosa. Although many factors have been postulated as etiological factors for RAU, the role of Helicobacter pylori as a causative agent of RAU remains controversial. We therefore investigated the association of H. pylori and RAU by a highly sensitive technique, nested polymerase chain reaction (PCR), in 22 patients with RAU with ages ranging from 12-36 years. Samples were brushed from the lesions and the dorsum of the tongue of each patient. In addition, samples from the dorsum of the tongue of 15 normal individuals with ages ranging from 13-40 years were used as controls. The results showed that only one sample from a lesion (4.5 %) and one sample from the tongue (4.5 %) of two different patients with RAU were positive for H. pylori. In the control group, 3 samples (20 %) were positive for H. pylori. These findings suggest that H. pylon does not play a role in the pathogenesis of RAU and the dorsum of the tongue may be a reservoir of H. pylori in some individuals. (J.

The effect of gastric Helicobacter pylori eradication on recurrent aphthous stomatitis

“…Recently, H. pylori has been considered as a causative factor for RAS as well as many other dermatological disorders such as urticaria, rosacea, lichen planus, Behçet's disease, and atopic dermatitis. It has been speculated that H. pylori may cause mucosal injury by inducing lymphocyte‐derived chemokines resulting local neutrophil accumulation and release of proteolytic enzymes and oxygen radicals 1–3 …”

“…Many genetical, immunological, physical, psychological, and microbiological factors have all been considered as causative subjects. In recent years, H. pylori has been suggested as one of the important etiological agents in the pathogenesis of RAS 1–4 …”

Recurrent Aphthous Stomatitis - A Review

“…Many facts have suggested that oral cavity is a reservoir of the organism. Oral cavity is a possible route of transmission to other sites (Anak Iamaroon et al, 2003). So the secondary source of infection was considered as oral cavity.…”

Impact of Helicobacter pylori on the clinical course of recurrent aphthous stomatitis

“…Histological similarities are shown between gastric and oral ulcers (21,26,27). In recent years, H. pylori has been proposed to be one of the important etiological agents in the pathogenesis of RAS (21,(26)(27)(28)(29)(30)(31). It has been showed that patients with recurrent oral ulcerations appear to suffer from active H. pylori infection in a high percentage up to 70.8% of cases (21).…”