Detection of Helicobacter pylori in raw bovine milk by fluorescence in situ hybridization (FISH)

Angelidis, Apostolos S.; Tirodimos, Ilias; Bobos, Mattheos; Kalamaki, Mary S.; Papageorgiou, Dimitrios; Arvanitidou, Malamatenia

doi:10.1016/j.ijfoodmicro.2011.09.007

Cited by 39 publications

(35 citation statements)

References 42 publications

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“…Osman, El‐Eragi, Musa, and El‐Magboul () exhibited that the incidence of H. pylori in raw milk samples of different parts of Sudan had a range of 7–38%. Similar results have been reported for the high incidence of H. pylori in dairy product samples of Japan (72.20%) (Fujimura et al, ), Greece (20.00%; Angelidis et al, ), Italy 1.80%; Bianchini et al, ), and Iran (16.00%; Safaei, Rahimi, Zandi, & Rashidipour, ).…”

Section: Resultssupporting

confidence: 84%

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

Ranjbar

Farsani

Dehkordi

2018

Journal of Food Safety

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Dairy products play a considerable role in transmission of Helicobacter pylori. The current study was done to assess phenotypic characters of antimicrobial resistance and genotyping pattern of vacA, cagA, and iceA alleles among the H. pylori strains isolated from dairy products. Eight‐hundred dairy samples were collected and cultured. Antimicrobial resistance and genotyping patterns were assessed using disk diffusion and polymerase chain reaction, respectively. Thirty‐one out of 800 (3.87%) traditional dairy product samples were positive for H. pylori. Cheese (11.53%) samples had the highest incidence of H. pylori, while yoghurt (1.11%) had the lowest. Helicobacter pylori strains harbored the highest incidence of resistance against ampicillin (93.54%), amoxicillin (93.54%), tetracycline (90.32%), erythromycin (80.64%), and metronidazole (77.41%). VacA s1a (90.32%), m1a (90.32%), s2 (80.64%) and m2 (77.19%), cagA (51.61%), and iceA1 (32.25%) were the most routinely identified genotypes. S1am1a (67.74%), s2m1a (64.51%), and s1am2 (51.61%) were the most routinely identified genotyping pattern. Simultaneous presence of vacA, cagA, and iceA genotypes in antimicrobial resistant H. pylori strains indicates important public health issue regarding the consumption of dairy products. However, additional researches are required to find molecular genetic homology and other epidemiological aspects of H. pylori in dairy products. Practical applications Due to the high consumption rate of dairy samples, they should have a high microbial quality. Foods with animal origins and especially dairy samples are considered as a probable source of Helicobacter pylori infections. The incidence of contamination of dairy samples by H. pylori is fairly high, and nearly all of the isolates exhibited resistance against several types of antimicrobials and harbored pathogenic vacA, cagA, and iceA genotypes, so the risk of probable foodborne diseases caused by H. pylori in such products should not be neglected. Additionally, dairy samples may act as a reservoir of H. pylori with ability to transfer antimicrobial resistance and virulent genotypes.

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Section: Resultssupporting

confidence: 84%

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

Ranjbar

Farsani

Dehkordi

2018

Journal of Food Safety

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“…This could be attributed to the fact that H. pylori can survive for short period of time in water [8, 20]. Moreover, the method employed for H. pylori isolation may lack sufficient sensitivity to recover very low numbers of H. pylori [2, 23, 26, 27]. …”

Section: Resultsmentioning

confidence: 99%

Detection of Helicobacter pylori in City Water, Dental Units′ Water, and Bottled Mineral Water in Isfahan, Iran

Bahrami

Rahimi

Safaei

2013

The Scientific World Journal

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Helicobacter pylori infection in human is one of the most common infections worldwide. However, the origin and transmission of this bacterium has not been clearly explained. One of the suggested theories is transmission via water. This study was conducted to determine the prevalence rate of H. pylori in tap water, dental units' water, and bottled mineral water in Iran. In the present study, totally 200 water samples were collected in Isfahan province and tested for H. pylori by cultural method and polymerase chain reaction (PCR) by the detection of the ureC (glmM) gene. Using cultural method totally 5 cultures were positive. Two out of 50 tap water samples (4%), 2 out of 35 dental units' water (5.8%) samples, and 1 out of 40 (2.5% ) from water cooler in public places were found to be contaminated with H. pylori. H. pylori ureC gene was detected in 14 (7%) of water samples including 5 tap water (10%), 4 dental units' water (11.4%), 1 refrigerated water with filtration, and 4 (10%) water cooler in public places samples. This may be due to the coccoid form of bacteria which is detected by PCR method.

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“…This could be attributed to the fact that H. pylori can survive for a short period of time in water . Moreover, the method employed for H. pylori isolation may lack sufficient sensitivity to recover very low numbers of H. pylori .…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori isolated from Iranian drinking water: vacA, cagA, iceA, oipA and babA2 genotype status and antimicrobial resistance properties

et al. 2016

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Despite the clinical importance of Helicobacter pylori in human gastric disorders, its exact route of transmission is still uncertain. Based on the contentious hypothesis and findings of previous investigations, water may play an important role in the transmission of H. pylori to humans. This study was carried out to investigate the vacA , cagA , oipA , iceA and babA2 genotype status and antimicrobial resistance properties of H. pylori strains isolated from the drinking water samples of four major provinces in Iran. A total of 400 drinking water samples were cultured and tested. H. pylori ‐positive strains were analyzed for the presence of various genotypes and antimicrobial resistance. Twelve of 400 (3%) water samples were positive for H. pylori . Samples from Isfahan province had the highest, while those from Shiraz had the lowest prevalence of H. pylori . The seasonal distribution was also determined, with the highest prevalence of bacteria in the summer season (7.36%). H. pylori strains harbored the highest levels of resistance against ampicillin (100%), erythromycin (75%), clarithromycin (75%), and trimethoprim (58.3%). The most commonly detected genotypes were vacAs1a (83.3%), vacAm1a (66.6%), vacAs2 (50%) and cagA (50%). The presence of similar genotypes in the H. pylori strains of drinking water and those of human clinical samples suggest that contaminated water maybe the sources of bacteria. Spiramycin and furazolidone are suggested for the treatment of cases of H. pylori infection.

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Detection of Helicobacter pylori in raw bovine milk by fluorescence in situ hybridization (FISH)

Cited by 39 publications

References 42 publications

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

Detection of Helicobacter pylori in City Water, Dental Units′ Water, and Bottled Mineral Water in Isfahan, Iran

Helicobacter pylori isolated from Iranian drinking water: vacA, cagA, iceA, oipA and babA2 genotype status and antimicrobial resistance properties

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