Detection of Helicobacter pylori by enzyme-linked immunosorbent assay of thermophilic helicase-dependent isothermal DNA amplification

Gill, Pooria; Amini, Mohsen; Ghaemi, Amir; Shokouhizadeh, L; Abdul-Tehrani, Hossein; Karami, Ali; Gilak, Armin

doi:10.1016/j.diagmicrobio.2007.05.005

Cited by 23 publications

(9 citation statements)

References 22 publications

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“…Several oligonucleotide probes and assays for the detection and differentiation of Helicobacter species have been developed for H. pylori (On 1996;Jiang et al 1998;Ozpolat et al 2000;Gill et al 2007;Guimaraes et al 2007) and other Helicobacter species such as H. bilis, H. bizzozeronii, ''Candidatus H. bovis,'' H. canis, H. felis, H. heilmannii strains 1-4, H. hepaticus, H. pullorum, H. salomonis, H. suis and H. trogontum (Stanley et al , 1994Dewhirst et al 1994;Battles et al 1995;Fox et al 1995Fox et al , 1998bMendes et al 1996;Germani et al 1997;De Groote et al 2000;Roosendaal et al 2000;Shen et al 2000;Choi et al 2001;Ge et al 2001;Bridgeford et al 2008). However it should be noted that the sensitivity and specificity of these probes and assays have not been validated against all species currently described in this genus.…”

Section: Detection Of Helicobacter Speciesmentioning

confidence: 99%

The Family Helicobacteraceae

et al. 2014

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Section: Detection Of Helicobacter Speciesmentioning

confidence: 99%

The Family Helicobacteraceae

et al. 2014

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“…used a ure C‐based PCR with the isothermal digoxigenin (DIG)‐labeling tHDA process. It allowed to detect the amplicons with a specific biotinylated DNA probe and gave excellent sensitivity and specificity compared to culture [16]. Chisholm and Owen used a multiplex PCR for H. pylori speciation and two real‐time probe hybridization assays for clarithromycin and tetracycline susceptibility testing.…”

Section: Invasive Testsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori

et al. 2008

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The different invasive and noninvasive diagnostic tests for Helicobacter pylori have been applied mainly in emerging countries. Molecular methods have been developed, especially a test for detection of H. pylori and its clarithromycin resistance directly from stools. The long-term effects of eradication on histologic lesions have been studied in a meta-analysis and the prognostic value of post-treatment in gastric mucosa-associated lymphoid tissue lymphoma has been assessed. An operating link for gastritis assessment (the OLGA staging) has also been published. Attempts to simplify the urea breath test protocol have been made, and new stool antigen tests have been proposed and compared to those previously available.

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“…Moreover, a novel one-tube isothermal reverse transcription-thermophilic HDA (RT-tHDA) system has been developed to detect RNA viruses, including enterovirus and ebola virus [76]. Thermophilic HDA in combination with enzyme-linked immunosorbent assay was also used by Gill et al [82,96] for the detection of Helicobacter pylori. In addition, they also developed a colorimetric method to detect H. pylori by using isothermal…”

Section: Applications Of Hda Assaymentioning

confidence: 99%

Alternative Molecular Tests for Virological Diagnosis

et al. 2012

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Several nucleic acid amplification techniques (NAATs), particularly PCR and real time PCR, are currently used in the routine clinical laboratories. Such approaches have allowed rapid diagnosis with a high degree of sensitivity and specificity. However, conventional PCR methods have several intrinsic disadvantages such as the requirement for temperature cycling apparatus, and sophisticated and costly analytical equipments. Therefore, amplification at a constant temperatures is an attractive alternative method to avoid these requirements. A new generation of isothermal amplification techniques are gaining a wide popularity as diagnostic tools due to their simple operation, rapid reaction and easy detection. The main isothermal methods reviewed here include loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), and helicase-dependent amplification (HDA). In this review, design criteria, potential of amplification, and application of these alternative molecular tests will be discussed and compared to conventional nucleic acid amplification techniques.

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Detection of Helicobacter pylori by enzyme-linked immunosorbent assay of thermophilic helicase-dependent isothermal DNA amplification

Cited by 23 publications

References 22 publications

The Family Helicobacteraceae

The Family Helicobacteraceae

Diagnosis of Helicobacter pylori

Alternative Molecular Tests for Virological Diagnosis

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