Diagnosis of <i>Helicobacter pylori</i>

Granström, Marta; Lehours, Philippe; Bengtsson, Carina; Mégraud, Françis

doi:10.1111/j.1523-5378.2008.00637.x

Cited by 26 publications

(21 citation statements)

References 61 publications

(60 reference statements)

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“…The rapid stool test is based on the same monoclonal antibody as the monoclonal EIA which was used for comparison. Our results confirmed the excellent performance of the monoclonal EIA both pre-and posttreatment in pediatric patients (8,9,15). All published trials with children using the monoclonal EIA consistently showed values of Ն95% for sensitivity and specificity.…”

Section: Discussionsupporting

confidence: 85%

Improved Performance of a Rapid Office-Based Stool Test for Detection of Helicobacter pylori in Children before and after Therapy

et al. 2009

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A modified version of a rapid office based one-step monoclonal immunoassay for detection of Helicobacter pylori antigen in stool samples from children was evaluated against biopsy specimen-based methods and compared to a monoclonal enzyme immunoassay using the same antigen. Blinded stool samples from 185 children (0.3 to 18.2 years) were investigated at the time of upper endoscopy prior to anti-H. pylori therapy; 62 children were H. pylori infected and 123 noninfected according to predefined reference standards. Samples obtained 6 to 8 weeks after anti-H. pylori therapy were available from 58 children (3.8 to 17.7 years) and were compared to results of the [ 13 C]urea breath test (14/58 were positive). The rapid stool tests were performed by two independent readers. Of 243 rapid tests performed, 1 (0.4%) was invalid for technical reasons. Equivocal results (very weak line) were reported 16 times by reader 1 and 27 times by reader 2. When equivocal results were considered positive, the two observers agreed on 76 positive and 160 negative results and disagreed on 7 samples (2.9%). The sensitivity was 90.8% for reader 1 and 85.5% for reader 2, and the specificity was 91.0% and 93.4%, respectively. The monoclonal enzyme immunoassay revealed a sensitivity and specificity of 94.7% and 97.6%, respectively. The modified chromatographic immunoassay is a good alternative in settings or situations when the monoclonal enzyme immunoassay or the [ 13 C]urea breath test are not available or feasible. In order to improve sensitivity, very weak lines should be considered positive test results.Several noninvasive methods are available for the diagnosis of H. pylori infection (5, 14). Serological tests are not appropriate, since they cannot distinguish between a present and previous infection and, in addition, they have a low sensitivity in children younger than 12 years of age (6, 13). The [ 13 C]urea breath test (UBT) is the preferred noninvasive diagnostic tool and gives excellent performance for both adults and children, but specificity decreases in very young and mentally disabled children who are not able to cooperate with the test procedure (10,11,25). So far, tests for detection of H. pylori antigen in stool samples are the only noninvasive diagnostic tools which do not show an age dependence for the diagnostic accuracy (14, 15). This makes stool tests very attractive, particularly for young children and for epidemiological studies. Several tests have been developed, but validation studies showed differences in performance. An enzyme immunoassay (EIA) based on polyclonal antibodies that was developed by the Meridian Company has been validated in several studies, with controversial results (17,20,24). Lack of accuracy is obviously related to intertest variability (19). In contrast, EIA based on monoclonal antibodies showed consistently excellent results, with very high sensitivity and specificity in both children and adults (15,21).A meta-analysis with head-to-head comparison has judged the monoclonal EIA superior to the po...

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Section: Discussionsupporting

confidence: 85%

Improved Performance of a Rapid Office-Based Stool Test for Detection of Helicobacter pylori in Children before and after Therapy

et al. 2009

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“…13,14 Trials conducted in various epidemiological settings consistently associated GC with OLGA stages III ⁄ IV. [15][16][17] Pepsinogens are proteolytic enzymes produced by gastrointestinal epithelia: pepsinogen I (PgI) is virtually found only in the gastric corpus, whereas pepsinogen II (PgII) is produced by both antral and oxyntic epithelia. Gastric mucosal atrophy results in a (progressive) decline in both serum PgI and the PgI ⁄ PgII ratios.…”

Section: Introductionmentioning

confidence: 99%

Gastritis OLGA‐staging and gastric cancer risk: a twelve‐year clinico‐pathological follow‐up study

Rugge¹,

Boni²,

Pennelli³

et al. 2010

Aliment Pharmacol Ther

233

180

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“…Invasive test are endoscopy, culture, molecular methods, 11 These sensitivity and specificity are not worse, but even better than those of other recent non-invasive methods. 10 A limitation of this method is that it does not discriminate between current and old infection as it is a serology test. 12 But, although the endoscopic biopsy and urea breath test are useful for active GI infection, they are not completely reliable for nondigestive disease.…”

Section: Discussionmentioning

confidence: 99%

Association between Helicobacter pylori infection and Posner–Schlossman syndrome

Choi

Kim

et al. 2009

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Objective: To investigate possible association between Helicobacter pylori infection and Posner-Schlossman syndrome. Methods: In a prospective study, 40 subjects with Posner-Schlossman syndrome were examined. As a control group, 73 subjects without Posner-Schlossman syndrome were selected for comparison with the study group. All participants underwent serologic analysis for the presence of H. pylori infection by an enzyme-linked immunosorbent assay. Positive rate of serum anti-H. pylori IgG was compared between Posner-Schlossman syndrome patients and control participants. Results: The rate of positive titre (415 U/ml) of H. pylori was 80.0% in the Posner-Schlossman syndrome group and 56.2% in control group, showing significant difference (Fischer's exact, P ¼ 0.014). Multivariate analysis adjusted for various systemic factors revealed that positive titre of anti-H. pylori IgG was a significant risk factor for Posner-Schlossman syndrome with an odds ratio, (4.84, 95.0% CI: 1.10-21.24, P ¼ 0.037). Conclusions: H. pylori infection occurred significantly more often in PosnerSchlossman syndrome patients. This study suggests that exposure to H. pylori infection is associated with Posner-Schlossman syndrome in Korea.

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Diagnosis of Helicobacter pylori

Cited by 26 publications

References 61 publications

Improved Performance of a Rapid Office-Based Stool Test for Detection of Helicobacter pylori in Children before and after Therapy

Improved Performance of a Rapid Office-Based Stool Test for Detection of Helicobacter pylori in Children before and after Therapy

Gastritis OLGA‐staging and gastric cancer risk: a twelve‐year clinico‐pathological follow‐up study

Association between Helicobacter pylori infection and Posner–Schlossman syndrome

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