Detection of Helicobacter Antigen in Stool Samples and Its Relation to H. Pylori Positive Cholecystitis in Egyptian Patients with Chronic Calcular Cholecystitis

Abstract

Evidences supporting the association between H. pylori infection and chronic cholecystitis could be found by using direct culture or staining of H. pylori in gallbladder tissues as well as indirect techniques. Stool antigen test has been widely used due to its non-invasive nature. Various stool antigen tests were developed to detect H. pylori using an enzyme immunoassay (EIA) based on monoclonal or polyclonal antibodies This study evaluated the frequency of H. pylori antigen in stool samples of patients with chronic calcular cholecystitis as regard gall bladder histopathological changes.-Fifty patients were included presented with symptomatic cholecystolithiasis recruited from the outpatient clinic of National Hepatology and Tropical Medicine Research Institute during 2014-2015. Full history and clinical examination and abdominal ultrasonography were performed. Stool samples were collected, prepared and examined for detection of H. pylori antigen. Cholecystectomy was done for all patients; 45 patients (90%) by laparoscopic Cholecystectomy and 5 patients (10%) by open surgery and removed gallbladders were submitted to pathology department for detection of H. pylori in tissue under microscope using Giemsa stain. The results showed that (82%) were females with mean age (42.6+1years). The mean BMI was (29+7.2) H. pylori-specific antigen in stool samples was detected in 40% of patients and38% were detected in patients; tissue, with significant correlation between H. pylori-specific antigen in stool and in tissue. Histopathological pictures infection in tissue were 68.4% mucosal erosions, 63.2% mucosal atrophy, 57.9% mucosal hyperplasia, 26.3% metaplasia, 42.1% musculosa hypertrophy, 26.3% fibrosis, but lymphoid aggregates were in 42.1% of cases.

<b><i>Helicobacter pylori</i></b> Infection in Africa: Update of the Current Situation and Challenges

“…Moosavian et al [55] and Demiray-Gurbuz et al [56] reported the rapid assessment of clarithromycin susceptibility of H. pylori in the biopsies of patients with dyspepsia by using fluorescence in situ hybridization. The stool antigen test (SAT) is a monoclonal or polyclonal antibody approach which has been widely adapted as a noninvasive method in the screening for HPI [57]. However, the monoclonal antibody based tests are superior to the polyclonal.…”