Detection of Heavy Metal Ions in Drinking Water Using a High-Resolution Differential Surface Plasmon Resonance Sensor

Abstract

We have built a high-resolution differential surface plasmon resonance (SPR) sensor for heavy metal ion detection. The sensor surface is divided into a reference and sensing areas, and the difference in the SPR angles from the two areas is detected with a quadrant cell photodetector as a differential signal. In the presence of metal ions, the differential signal changes due to specific binding of the metal ions onto the sensing area coated with properly selected peptides, which provides an accurate real-time measurement and quantification of the metal ions. Selective detection of Cu2+ and Ni2+ in the ppt-ppb range was achieved by coating the sensing surface with peptides NH2-Gly-Gly-His-COOH and NH2-(His)6-COOH. Cu2+ in drinking water was tested using this sensor.

Screening of Peptide Sequences Cognitive of Pb2+by Biopanning

“…같은 분광학적 방법들이 이용되고 있는데, 시료의 전처리가 필요하거나 고가의 장비가 요구되는 단점이 있다 [1]. 따라 서 간단하고 비용이 적게 들면서 실시간으로 측정이 가능한 방법의 개발이 요구되고 있다.…”

Detection of arsenic in groundwater using a surface plasmon resonance sensor

“…The SPR set-up was described elsewhere [25,28]. Briefly, it is based on the Kretschmann configuration, in which a ppolarized laser beam (λ = 635 nm) is focused through a prism onto a metal film placed on the prism.…”

“…3B), the SPR angle decreases, which is opposite from the case of dTGluc-coated sensing surfaces. Typically, a binding event increases the index of refraction and therefore causes an increase in the SPR angle [25]. However, a binding event can cause a decrease in the effective thickness of the sensing molecules due to conformational changes in the sensing molecules, which will decrease the SPR angle.…”

“…SPR-based technology is an accepted and established tool for investigation of biological processes, adsorption phenomena, characterization of binding properties of antibodies and other biological molecules, gene analysis and drug discovery [17][18][19][20][21][22][23][24]. Recently, we have shown that a high resolution SPR can be used to detect and quantify heavy metal ions in drinking water [25]. In the present work, we have developed a method to functionalize the SPR sensing surfaces for selectively and sensitively detecting arsenic in groundwater, and demonstrating a potentially simple and costeffective device for on-site screening test of arsenic in surface and groundwater.…”

Ultrasensitive system for the real-time detection of H 2 O 2 based on strong coupling in a bioplasmonic system

“…Sensing using plasmonic resonances in thin films, nanoparticles and nanostructures has been a key area of research for scientists since it opens up avenues for detection of various biomolecules and biological species in real time and with high specificity [1][2][3][4][5][6][7]. Even though standard localized Plasmon resonance (LSPR) sensing techniques using different plasmonic nanoparticles give highly sensitive specific real time information either in-vivo or in-vitro, they suffer from the drawback that the analyte to be sensed must cause enough refractive index change to be measurable.…”