Detection of heart-type fatty acid-binding protein (h-FABP) using piezoresistive polymer microcantilevers functionalized by a dry method

Agarwal, Deepti; Prasad, Abhinav; Vinchurkar, Madhuri; Gandhi, Sahir; Prabhakar, Deepika; Mukherji, Soumyo; Rao, V. Ramgopal

doi:10.1007/s13204-018-0723-y

Cited by 18 publications

(9 citation statements)

References 48 publications

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“…In 2015, Liu et al [ 158 ] modified a dynamic microcantilever with the biotin–antibiotin system to detect ricin protein. In 2018, the group of Agarwal [ 159 ] detected a heart-type fatty acid-binding protein (h-FABP) in a trace amount (100 ng/mL) by employing a piezoresistive SU-8/CB microcantilever platform for the first time. In 2020, Dilip et al [ 160 ] tested cardiac troponin-I by using a a SiN–PolySi–SiO 2 composite microcantilever modified with HIgG and Anti-HIgG.…”

Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

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When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application.

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Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

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“…Moreover, the polymeric nature of the MIP layer provided high stability of the proposed biosensor at ambient temperature up to 3 weeks, depicting its excellent storage ability than the previously reported antibody-based biosensors that are usually stored at 4 °C. 45,76,77 However, the performance of the biosensor in undiluted serum samples and the electrochemical setup for point-of-care applications need to be improved in future investigations. As MIP biosensors are more stable, more efficient, and more scalable, than antibody-based biosensors at ambient temperature and in clinical environments, this biosensor combination can be considered for fast, sensitive, and affordable detection of proteins in future studies.…”

Section: ■ Conclusionmentioning

confidence: 99%

Gold Nano/Micro-Islands Overcome the Molecularly Imprinted Polymer Limitations to Achieve Ultrasensitive Protein Detection

et al. 2021

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Here, we report on an electrochemical biosensor based on core–shell structure of gold nano/micro-islands (NMIs) and electropolymerized imprinted ortho-phenylenediamine (o-PD) for detection of heart-fatty acid binding protein (H-FABP). The shape and distribution of NMIs (the core) were tuned by controlled electrodeposition of gold on a thin layer of electrochemically reduced graphene oxide (ERGO). NMIs feature a large active surface area to achieve a low detection limit (2.29 fg mL–1, a sensitivity of 1.34 × 1013 μA mM–1) and a wide linear range of detection (1 fg mL–1 to 100 ng mL–1) in PBS. Facile template H-FABP removal from the layer (the shell) in less than 1 min, high specificity against interference from myoglobin and troponin T, great stability at ambient temperature, and rapidity in detection of H-FABP (approximately 30 s) are other advantages of this biomimetic biosensor. The electrochemical measurements in human serum, human plasma, and bovine serum showed acceptable recovery (between 91.1 ± 1.7 and 112.9 ± 2.1%) in comparison with the ELISA method. Moreover, the performance of the biosensor in clinical serum showed lower detection time and limit of detection against lateral flow assay (LFA) rapid test kits, as a reference method. Ultimately, the proposed biosensor based on the core–shell structure of gold NMIs and MIP opens interesting avenues in the detection of proteins with low cost, high sensitivity and significantstability for clinical applications.

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“…Nanomechanical systems can exhibit extremely low mechanical compliances translating biomolecular recognition events into measurable displacements and vibrational properties of the nanomechanical structures when adsorption of biomolecules or biomolecular interactions take plane on the nanomechanical system surface (Arlete et al, 2011;Boisen et al, 2011;Buchapudi et al, 2011;Calleja et al, 2012;Consortium, 2012;Eom et al, 2011). Microcantilever sensors have been used for a wide range of applications such as the detection of cancer cells (Etayash et al,2015;Chen et al, 2016;Pandya et al, 2015), proteins (Wu et al,2001;Lee et al,2005;Arntz et al, 2003;Kosaka et al, 2013;Kosaka et al, 2014;Kosaka et al, 2017;Agarwal et al,2018), DNA (Calleja et al,2005;Biswal et al,2006;Mertens et al, 2008;Dominguez et al, 2015) mRNA (Duffy et al, 2018) and bacteria (Longo et al, 2013;Wang 2014;Kasas et al, 2015;Malvar et al, 2016).…”

Section: Introductionmentioning

confidence: 99%