Detection of Hæmoglobin, Hæmoglobin–Haptoglobin Complexes and other Substances with Peroxidase Activity after Zone Electrophoresis

Phenotype and gene frequencies are presented for eight polymorphic systems among the Nubians of South Egypt, namely, acid phosphatase, glucose-6-phosphate dehydrogenase, adenylate kinase, 6-phosphogluconate dehydrogenase, esterase D, phosphoglucomutase I, peptidase A, and haptoglobin. Eleven systems, namely, albumin, ceruloplasmin, hemoglobin, lactate dehydrogenase, isocitrate dehydrogenase, phosphohexose isomerase, malate dehydrogenase, peptidase B and C, phosphoglucomutase II, and transferrin were found to be monomorphic. A single electrophoretic variant of phosphohexose isomerase was observed.

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“…Haptoglobin was visualized by the method of O wen el at. [16], and ceruloplasmin by the method of Shreffler et at. [18].…”

Section: Methodsmentioning

confidence: 99%

The Nubians of Kom Ombo: Serum and Red Cell Protein Types

Bertin¹,

Harris²,

Ferrell³

et al. 1978

Hum Hered

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“…Electrophoresis on starch block followed the method of Kunkel (1954), and in starch gel the method of Smithies (1955) using either the discontinuous buffer system (Poulik, 1957) or a phosphate buffer system . The haemoglobin zones in starch gel were detected with o-dianisidine (Owen et al, 1958). After separation of the haemoglobin zones on starch block the appropriate zone was cut out and the haemoglobin eluted from the starch with 0.02M sodium chloride.…”

Section: Methodsmentioning

confidence: 99%

Four Haemoglobins in One Individual

Raper¹,

Gammack²,

Huehns³

et al. 1960

BMJ

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“…None of these four enzymes is discussed further. Myoglobin (together with other proteins with catalase activity) was stained with o-dianisidine as described by Owen, Silberman & Got (1958).…”

Section: Hexmentioning

confidence: 99%

Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species

Scopes

1968

Biochemical Journal

170

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1. Details of an improved method for starch-gel electrophoresis of water-soluble muscle proteins are given. 2. Methods are described for detecting enzyme activities on the starch gel after electrophoresis, by using pieces of filter paper. 3. Compositions of incubation mixtures suitable for detecting any of the enzymes of glycolysis, and certain other enzymes, are given. 4. A comparison of the various enzymes in extracts of several muscles from one rabbit was made; most differences are quantitative only. 5. A detailed comparison of the mobilities of various enzymes in extracts of muscles from a wide variety of species was made. Each species was found to have a characteristic pattern of proteins on the starch gel, and the mobilities of individual enzymes varied considerably. 6. Potential uses and extensions of the methods are discussed.

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Detection of Hæmoglobin, Hæmoglobin–Haptoglobin Complexes and other Substances with Peroxidase Activity after Zone Electrophoresis

Cited by 141 publications

References 6 publications

The Nubians of Kom Ombo: Serum and Red Cell Protein Types

The Nubians of Kom Ombo: Serum and Red Cell Protein Types

Four Haemoglobins in One Individual

Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species

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