We prepared and investigated a nanomaterial consisting of calmodulin functionalized with fluorescent dyes and gold nanoparticles and we have shown that calmodulin covalently linked to an excited fluorescent dye and gold nanoparticle can stimulate a surface-plasmon-coupled emission, resulting in a strong fluorescence enhancement. Herewith, calmodulin molecules were functionalized and stabilized with gold nanoparticles and fluorescent dyes in liquid and solid environments. The photo-optical properties of the proposed novel nanomaterial are promising for the development of a simple and effective method for the targeting, labelling and visualization of calmodulin-mediated processes, such as proliferation, inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, etc.