Detection of glutathione based on MnO2 nanosheet-gated mesoporous silica nanoparticles and target induced release of glucose measured with a portable glucose meter

Abstract

The authors describe a novel method for the determination of glutathione (GSH). Detection is based on target induced release of glucose from MnO nanosheet-gated aminated mesoporous silica nanoparticles (MSNs). In detail, glucose is loaded into the pores of MSNs. Negatively charged MnO nanosheets are assembled on the MSNs through electrostatic interactions. The nanosheets are reduced by GSH, and this results in the release of glucose which is quantified by using a commercial electrochemical glucose meter. GSH can be quantified by this method in the 100 nM to 10 μM concentration range, with a 34 nM limit of detection. Graphical abstract Glucose is loaded into the pores of mesoporous silica nanoparticles (MSNs). MnO nanosheets are assembled on MSNs through electrostatic interactions. Glutathione (GSH) can reduce the nanosheets, and this results in the release of glucose which is quantified by using a commercial glucose meter.

NIR-II-responsive AuNRs@SiO2–RB@MnO2nanotheranostic for multimodal imaging-guided CDT/PTT synergistic cancer therapy

“…Subsequently, 100 mL of 2 mM RB was dispersed into the above solution, and the resulting mixture was then gently shaken for 16 h at room temperature, the RB molecules were diffused into the pores of the amine-modified AuNRs@SiO 2 . 26,66 MnO 2 nanosheets were synthesized as reported method from reference.66,67 In a 50 mL round-bottom flask, 5 mL of 0.3 M MnCl 2 aqueous solution was mixed with 10 mL of 0.6 M tetramethylammonium hydroxide aqueous solution (3 wt% H 2 O 2 ) for 15 s. After stirring vigorously for 12 h at room temperature, the solution was filtered through a 0.45 mm membrane, washed several times with alternating ultrapure water and methanol, and dried at 50 1C. The product was dispersed in water, and the resulting suspension was treated ultrasonically for 10 h to obtain a brown colloid.…”

SERS Sensors Based on Aptamer-Gated Mesoporous Silica Nanoparticles for Quantitative Detection of Staphylococcus aureus with Signal Molecular Release

“…Similarly, the particle size of MSNs can be controlled between 50 and 300 nm; the outer surface and inner pore surface of MSNs are easy to be functionalized, and the volume and release behavior of cargos can be better controlled by surface functionalization. Furthermore, studies have proved the exceptional biocompatibility and low cytotoxicity of MSNs. , The large specific surface area of MSNs provides sufficient space sites and makes it possible to load large numbers of cargos by different gates and diverse design strategies . MSNs are promising candidates for loading a wide range of substances, which has attracted significant attention in developing “turn-off–on” sensing platforms.…”

“…28,29 The large specific surface area of MSNs provides sufficient space sites and makes it possible to load large numbers of cargos by different gates and diverse design strategies. 30 MSNs are promising candidates for loading a wide range of substances, which has attracted significant attention in developing "turn-off−on" sensing platforms. Tang et al have designed a novel fluorescence immunoassay method based on target-induced cargo release from protein-gated carbohydrate-functionalized magnetic MSNs and successfully realized the detection of aflatoxin.…”

Mesoporous Silica Nanoparticles in Chemical Detection: From Small Species to Large Bio-Molecules

“…Silica-based nanomaterials are also used in peptides and proteins’ detection. That, F. Qu et al [ 74 ] reported the use of mesoporous silica nanoparticles gated with a MnO 2 nanosheet for detecting glutation (GHS). Aminated mesoporous silica nanoparticles were loaded with glucose, and then the pores were caped with negatively charged MnO 2 nanosheets through electrostatic interactions.…”