Detection of GB virus C/hepatitis G virus in semen and saliva of HIV type-1 infected men

Bourlet, Thomas; Berthelot, Philippe; Grattard, Florence; Genin, C; Lucht, F.; Pozzetto, Bruno

doi:10.1046/j.1469-0691.2002.00425.x

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…According to French legislation, sperm contaminated with human immunodeficiency virus type 1 (HIV-1) must be frozen after migration on a density gradient, in order to check the absence of HIV-1 viral RNA, in either seminal plasma or sperm fraction or these combined, by reversetranscriptase polymerase chain reaction (RT-PCR) [1][2][3]. In most reports, density gradient centrifugation has been shown to considerably reduce viral load in the migrated sperm fraction of HIV-1-infected patients [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Frainais

Vialard

Rougier

et al. 2010

J Assist Reprod Genet

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Introduction According french legislation, sperm freezing/ thawing procedures are used to prevent ART contaminations in couple with HIV-1 infected men. We determined sperm nuclear fragmentation rate before and after selection and freezing/thawing in HIV-1 14 patients.Methods Two groups of patients were studied: 20 control patients with normal sperm (group 1) and without viral infection and 20 fertile treated HIV-1 patients (group 2). DNA fragmentation was evaluated using terminal uridine nick end labeling, before and after gradient selection, and after cryopreservation and thawing procedures. Results DNA fragmentation rates in fresh semen were increased in HIV patients (6.38% vs 3.39%) (p<0.05) compared with control patients. After sperm migration, fragmentation rates were significantly lower (p<0.0001) in the two groups compared with fresh sperm rates. After freezing/thawing, values were similar to those of fresh semen with an increased rate (p<0.01) for HIV-1 patients, with respectively 3.40% and 5.18% rates in control and infected patients. HIV-1-infected patients treated by antiretroviral therapy showed a significant increase in sperm DNA fragmentation in fresh sperm and also after freezing/ thawing procedures, but these two fragmentation rates were not significantly different. Conclusion So, freezing/thawing procedures do not seem to impair sperm DNA and preserve probability of conception for couples with HIV-1 infected men.

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Section: Introductionmentioning

confidence: 99%

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Frainais

Vialard

Rougier

et al. 2010

J Assist Reprod Genet

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Le virus GB-C ou virus « dit » de l’hépatite G est-il impliqué en pathologie humaine ?

Chams¹,

Fournier‐Wirth²,

Chabanel³

et al. 2003

Transfusion Clinique et Biologique

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GB Virus Type C Coinfection in HIV‐Infected African Mothers and Their Infants, KwaZulu Natal, South Africa

et al. 2004

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GB virus type C (GBV-C) infection was studied in a convenience sample of 75 antiretroviral (ART)-naive African mothers with human immunodeficiency virus infection and their infants. GBV-C RNA was extracted from serum and amplified by reverse-transcriptase polymerase chain reaction. Twenty-seven (36%) of these 75 HIV-infected women tested positive for GBV-C RNA. To study transmission dynamics, we chose a random subsample of 20 of these women and their infants. In this cohort, there was evidence of postnatal transmission of GBV-C; however, it was not possible to demonstrate evidence of in utero or intrapartum transmission. In this pilot observational study, transmission of HIV from mother-to-infant occurs independently of the GBV-C infection status of the mother. The immunological indices measured tend to suggest an association with protection and or delayed progression of HIV disease in GBV-C-infected mothers.

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Detection of GB virus C/hepatitis G virus in semen and saliva of HIV type-1 infected men

Cited by 5 publications

References 24 publications

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients

Le virus GB-C ou virus « dit » de l’hépatite G est-il impliqué en pathologie humaine ?

GB Virus Type C Coinfection in HIV‐Infected African Mothers and Their Infants, KwaZulu Natal, South Africa

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