Detection of Four Major Bacterial Potato Pathogens

Kerr, Ellen M.; Cahill, Greig; Fraser, K.

doi:10.1007/978-1-59745-062-1_9

Cited by 1 publication

(1 citation statement)

References 12 publications

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“…The pel ADE-specific primers, ADE1 (5-ATC AGA AAG CCC GCA GCC AGA T-3) and ADE2 (5-CTG TGG CCG ATC AGG ATG GTT TTG TCG TGC-3), amplified a 420-bp pectate lyase-encoding gene fragment from all E. chrysanthemi strains tested, but not from any other soft-rot Erwinia (Charkowshi 2006). DNA was amplified with 25 cycles at 94°C for 1 min and 72°C for 2 min, with a final elongation step at 72°C for 8 min (Kerr et al 2009).…”

Section: Pcr Identificationmentioning

confidence: 99%

Soft rot of root chicory in Hokkaido and its causal bacteria

et al. 2013

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In August 2010, bacterial soft rot was found on root chicory (Cichorium intybus var. sativum) in Hokkaido, Japan. Severely infected plants in fields were discolored, had wilted foliage, and black necrosis of petioles near the crown. Wilted leaves subsequently collapsed and died, forming a dry, brown or black rosette. The root and crown became partially or wholly soft-rotted. Slimy masses on infected areas of roots, turned dark brown or black. Gram-negative, rod-shaped, peritrichously flagellated, facultatively anaerobic bacteria were exclusively isolated from rotted roots, and typical symptoms were reproduced after inoculation with the strains. The bacteria were identified as Dickeya dianthicola, Pectobacterium carotovorum subsp. carotovorum, and Pectobacterium carotovorum subsp. odoriferum based on further bacteriological characterization and the sequence analysis of the malate dehydrogenase gene and 16S rRNA gene. These bacteria should be included with the previously reported Dickeya (=Erwinia) chrysanthemi in Saitama Prefecture, Japan, as causal pathogens of bacterial wilt of chicory

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Section: Pcr Identificationmentioning

confidence: 99%