Soft rot of root chicory in Hokkaido and its causal bacteria

Lan, Wei Wei; Nishiwaki, Yoshie; Akino, Seishi; Kondo, Norio

doi:10.1007/s10327-013-0440-z

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…Phenotypic diversity among P. c. subsp. odoriferum strains was also documented by Lan et al (2013). The atypical phenotypic features of some P. c. subsp.…”

Section: Discussionmentioning

confidence: 94%

“…P. c. subsp. odoriferum was first isolated in western Europe (Belgium, France, and Switzerland) (Gallois et al 1992;Samson et al 1980) and has since been reported in Poland, southern Europe (Gallelli et al 2009), Africa (Yahiaoui-Zaidi et al 2010), and Asia (Nazerian et al 2011;Thapa et al 2011;Lan et al 2013). Although P. c. subsp.…”

Section: Discussionmentioning

confidence: 99%

“…and Japan (Lan et al 2013) but can cause disease on hyacinth, celery, leek, and sugar beet (Gallois et al 1992;Gardan et al 2003). In recent years, strains of this subspecies were also isolated from okra in Malaysia (Nazerian et al 2011), artichokes in Italy (Gallelli et al 2009), Chinese cabbage in Korea (Thapa et al 2011), and potato in Korea (Thapa et al 2011) and Algeria (Yahiaoui-Zaidi et al 2010).…”

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confidence: 99%

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Characterization of Pectobacterium carotovorum subsp. odoriferum causing soft rot of stored vegetables

Waleron

Łojkowska

2014

Eur J Plant Pathol

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Pectobacterium carotovorum subsp. odoriferum has been generally considered to have a narrow host range and has been isolated most often from chicory. Research was conducted to identify 91 Pectobacterium spp. strains isolated from different vegetables in Europe, North and South America, Asia, and Africa, and to compare their ability to cause disease in chicory and potato. Among the 91 strains, 22 strains from Europe were identified as P. c. subsp. odoriferum. Based on phylogenetic analysis of 16S rDNA, recA, and rpoS gene sequences, strains isolated from stored vegetables clustered together with the type strain of P. c. subsp. odoriferum and clustered separately from the P. c. subsp. carotovorum isolates. Eleven strains previously identified as P. c. subsp. carotovorum were reclassified as P. c. subsp. odoriferum. All P. c. subsp. odoriferum isolates were able to cause soft rot symptoms on chicory and potato. Moreover, the symptoms on potatoes were more severe at temperatures from 15 to 37°C with P. c. subsp. odoriferum isolates than with P. atrosepticum or P. c. subsp. carotovorum isolates. Tissue maceration by P. c. subsp. odoriferum isolates was highest at 28°C, and at that temperature tissue maceration was two-times greater for P. c. subsp. odoriferum isolates than for P. c. subsp. carotovorum isolates. Symptoms on inoculated chicory leaves were more severe with P. c. subsp. odoriferum (regardless of origin) than with other subspecies or species. To our knowledge, this is the first report that P. c. subsp. odoriferum occurs on a wide range of vegetables and has the ability to cause soft rot during potato storage.

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“…Phenotypic diversity among P. c. subsp. odoriferum strains was also documented by Lan et al (2013). The atypical phenotypic features of some P. c. subsp.…”

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of Pectobacterium carotovorum subsp. odoriferum causing soft rot of stored vegetables

Waleron

Łojkowska

2014

Eur J Plant Pathol

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“…Throughout these countries, D . dianthicola has a wide host range [6, 12, 13], but potato is considered the main host [14, 15]. Infected potato plants exhibit wilting, dwarfing, and wet, oozy, black stems [3].…”

Section: Introductionmentioning

confidence: 99%

Development of a robust, field-deployable loop-mediated isothermal amplification (LAMP) assay for specific detection of potato pathogen Dickeya dianthicola targeting a unique genomic region

et al. 2019

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Destructive maceration, a wide host range, and longevity in non-plant substrates has established Dickeya dianthicola (blackleg of potato) as a significant threat to potato industries worldwide. To protect these businesses, a specific and sensitive point-of-care D . dianthicola detection tool is necessary. We have developed a loop-mediated isothermal amplification (LAMP) assay for specific, sensitive, and rapid detection of D . dianthicola , which can be streamlined for point-of-care use. The developed LAMP assay targets a unique gene, alcohol dehydrogenase , of D . dianthicola . Assay specificity was assessed using strains present in inclusivity (16 D . dianthicola strains) and exclusivity panels (56 closely related, potato pathogenic, and other bacterial strains). Amplification with strains of inclusivity panel occurred, and cross-reactivity with non-target DNA was not observed. The limit of detection (LOD) was 10 CFU/ml when dilutions were made before isolating the genomic DNA; however, LOD was determined as 1 pg using 10-fold serially diluted D . dianthicola genomic DNA. Similar LOD of 1 pg was observed when serially diluted target genomic DNA was mixed with host genomic DNA. LOD (1 pg) was also calculated with 10-fold serially diluted synthetic DNA fragments containing primer target sites. Naturally and artificially inoculated plant samples were used for field adaptability tests with the field-deployable Optigene Plant Material Lysis Kit and a heat block (65°C); the results were obtained within 20 minutes. Despite the lack of method precision, no false positives or false negatives were observed. Therefore, with prepared reactions and a steady heat source, this assay can be used for rapid point-of-care detection, which is imperative for quarantine, eradication, disease management, and border protection.

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“…Carrot with soft rot disease. Adapted from Lindsey du Toit, Washington State University, North Carolina Cooperative Extension Sevice ( Lan et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Classification and Taxonomy of Vegetable Macergens

Aremu

Babalola

2015

Front. Microbiol.

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Macergens are bacteria capable of releasing pectic enzymes (pectolytic bacteria). These enzymatic actions result in the separation of plant tissues leading to total plant destruction. This can be attributed to soft rot diseases in vegetables. These macergens primarily belong to the genus Erwinia and to a range of opportunistic pathogens namely: the Xanthomonas spp., Pseudomonas spp., Clostridium spp., Cytophaga spp., and Bacillus spp. They consist of taxa that displayed considerable heterogeneity and intermingled with members of other genera belonging to the Enterobacteriaceae. They have been classified based on phenotypic, chemotaxonomic and genotypic which obviously not necessary in the taxonomy of all bacterial genera for defining bacterial species and describing new ones These taxonomic markers have been used traditionally as a simple technique for identification of bacterial isolates. The most important fields of taxonomy are supposed to be based on clear, reliable and worldwide applicable criteria. Hence, this review clarifies the taxonomy of the macergens to the species level and revealed that their taxonomy is beyond complete. For discovery of additional species, further research with the use modern molecular methods like phylogenomics need to be done. This can precisely define classification of macergens resulting in occasional, but significant changes in previous taxonomic schemes of these macergens.

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Soft rot of root chicory in Hokkaido and its causal bacteria

Cited by 10 publications

References 22 publications

Characterization of Pectobacterium carotovorum subsp. odoriferum causing soft rot of stored vegetables

Characterization of Pectobacterium carotovorum subsp. odoriferum causing soft rot of stored vegetables

Development of a robust, field-deployable loop-mediated isothermal amplification (LAMP) assay for specific detection of potato pathogen Dickeya dianthicola targeting a unique genomic region

Classification and Taxonomy of Vegetable Macergens

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