Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis

He, Shiyu; Huang, Yanzhi; Ma, Yingwei; Yu, Haoyan; Pang, Bo; Liu, Xingxing; Yin, Caihong; Wang, Xiaomu; Wei, Yuan; Tian, Yuling; Zhao, Chao; Xu, Kun; Wang, Juan; Lv, Chunping; Song, Xiaohong; Jin, Minghua

doi:10.1002/biot.202100335

Cited by 15 publications

(10 citation statements)

References 29 publications

(22 reference statements)

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“…This technique has been coupled with multiplex PCR and capillary electrophoresis to allow for simultaneous detection of Salmonella enterica subsp. enterica serovar Typhimurium, E. coli O157:H7, L. monocytogenes , and Staphylococcus aureus in artificially contaminated food products (biscuit, pear juice, milk, and chicken sausages) in the absence of pre‐enrichment step (He et al., 2022). In milk samples, the reported limit of detection reached 10 CFU/mL for each bacteria (He et al., 2022).…”

Section: Nucleic Acid‐based Amplification Methods For Targeted Detect...mentioning

confidence: 99%

“…enterica serovar Typhimurium, E. coli O157:H7, L. monocytogenes , and Staphylococcus aureus in artificially contaminated food products (biscuit, pear juice, milk, and chicken sausages) in the absence of pre‐enrichment step (He et al., 2022). In milk samples, the reported limit of detection reached 10 CFU/mL for each bacteria (He et al., 2022). Overall, the use of nanocarbon dots and magnetic beads for direct non‐specific extraction of bacteria increases the throughput of the multiplex‐PCR detection system.…”

Section: Nucleic Acid‐based Amplification Methods For Targeted Detect...mentioning

confidence: 99%

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Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Hadi

Rapp

Dhawan

et al. 2023

Comp Rev Food Sci Food Safe

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The global food demand is expected to increase in the coming years, along with challenges around climate change and food security. Concomitantly, food safety risks, particularly those related to bacterial pathogens, may also increase. Thus, the food sector needs to innovate to rise to this challenge. Here, we discuss recent advancements in molecular techniques that can be deployed within various foodborne bacteria surveillance systems across food settings. To start with, we provide updates on nucleic acid-based detection, with a focus on polymerase chain reaction (PCR)-based technologies and loop-mediated isothermal amplification (LAMP). These include descriptions of novel genetic markers for several foodborne bacteria and progresses in multiplex PCR and droplet digital PCR.The next section provides an overview of the development of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins systems, such as CRISPR-Cas9, CRISPR-Cas12a, and CRISPR-Cas13a, as tools for enhanced sensitive and specific detection of foodborne pathogens. The final section describes utilizations of whole genome sequencing for accurate characterization of foodborne bacteria, ranging from epidemiological surveillance to model-based predictions of bacterial phenotypic traits through genome-wide association studies or machine learning.

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Section: Nucleic Acid‐based Amplification Methods For Targeted Detect...mentioning

confidence: 99%

Section: Nucleic Acid‐based Amplification Methods For Targeted Detect...mentioning

confidence: 99%

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Hadi

Rapp

Dhawan

et al. 2023

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

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“…Multiple DNA fragments amplified in the same reaction system can simultaneously detect multiple foodborne pathogens, reduce the number of experimental operations, shorten the detection time, and save reagents ( Ma et al, 2020a ; Yang et al, 2020a ). He et al (2022) developed a detection system based on magnetic separation, mPCR, and capillary electrophoresis (CE) technologies for the simultaneous detection of four foodborne pathogens. The detection limit for bacterial DNA reached 10 −5 –10 −7 ng/μl and in the analysis of mocked food samples, the assay showed good sensitivity for bacterial detection ranging from 10 1 to 10 5 CFU/ml with excellent specificity.…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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“…coli O157.H7, S. typhimurium , and L. monocytogenes , and then combined multiplex PCR and capillary electrophoresis to improve detection sensitivity. With detection limits of 10 –5 –10 –7 ng/μL for bacterial DNA, the assay shows good sensitivity for bacterial detection in the range 10 1 –10 5 CFU/mL in the analysis of simulated food samples with excellent specificity . Huang et al used phage-bound MBs (pMB) as Salmonella bioidentifiers to pre-enrich Salmonella in food matrixes; then, DNA was extracted from bacteria–pMB complexes for real-time PCR detection (Figure D).…”

Section: Combination Technologies For Rapid Detection Of Microorganismsmentioning

confidence: 99%

“…With detection limits of 10 −5 −10 −7 ng/μL for bacterial DNA, the assay shows good sensitivity for bacterial detection in the range 10 1 −10 5 CFU/mL in the analysis of simulated food samples with excellent specificity. 111 Huang et al used phage-bound MBs (pMB) as Salmonella bioidentifiers to pre-enrich Salmonella in food matrixes; then, DNA was extracted from bacteria−pMB complexes for real-time PCR detection (Figure 3D). When the Salmonella concentration in milk and lettuce ranged from 30 to 3 × 10 7 CFU/mL, the cycle threshold value was linearly related to the bacterial concentration, and the whole assay could be completed within 1.5 h. 112 4.3.…”

Section: Combination Technologies For Rapid Detection Of Microorganismsmentioning

confidence: 99%

Magnetic Nanoseparation Technology for Efficient Control of Microorganisms and Toxins in Foods: A Review

Kang

Shi

Sun

et al. 2022

J. Agric. Food Chem.

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Outbreaks of foodborne diseases mediated by food microorganisms and toxins remain one of the leading causes of disease and death worldwide. It not only poses a serious threat to human health and safety but also imposes a huge burden on health care and socioeconomics. Traditional methods for the removal and detection of pathogenic bacteria and toxins in various samples such as food and drinking water have certain limitations, requiring a rapid and sensitive strategy for the enrichment and separation of target analytes. Magnetic nanoparticles (MNPs) exhibit excellent performance in this field due to their fascinating properties. The strategy of combining biorecognition elements with MNPs can be used for fast and efficient enrichment and isolation of pathogens. In this review, we describe new trends and practical applications of magnetic nanoseparation technology in the detection of foodborne microorganisms and toxins. We mainly summarize the biochemical modification and functionalization methods of commonly used magnetic nanomaterial carriers and discuss the application of magnetic separation combined with other instrumental analysis techniques. Combined with various detection techniques, it will increase the efficiency of detection and identification of microorganisms and toxins in rapid assays.

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Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis

Cited by 15 publications

References 29 publications

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Magnetic Nanoseparation Technology for Efficient Control of Microorganisms and Toxins in Foods: A Review

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