Detection of foodborne pathogens using novel vertical capacitive sensors

Abdelhamid, Hamdy; Morsy, Omar E.; El-Shibiny, Ayman; Abdelbaset, Reda

doi:10.1016/j.aej.2021.09.016

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…However, this dimension is proposed due to TSMC 130 nm technology limitations at metal 7. In addition, we provide CSM without a passivation layer to improve sensitivity, as demonstrated by past laboratory experiments 18 , 19 . The proposed microchannel's height, width, and length are 40 µm, 80 µm, and 200 µm, respectively.…”

Section: Methodsmentioning

confidence: 97%

CMOS based capacitive sensor matrix for characterizing and tracking of biological cells

Abdelbaset

El-Sehrawy

Morsy

et al. 2022

Sci Rep

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The characterization and tracking of biological cells using biosensors are necessary for many scientific fields, specifically cell culture monitoring. Capacitive sensors offer a great solution due to their ability to extract many features such as the biological cells' position, shape, and capacitance. Through this study, a CMOS-based biochip that consists of a matrix of capacitive sensors (CSM), utilizing a ring oscillator-based pixel readout circuit (PRC), is designed and simulated to track and characterize a single biological cell based on its aforementioned different features. The proposed biochip is simulated to characterize a single Hepatocellular carcinoma cell (HCC) and a single normal liver cell (NLC). COMSOL Multiphysics was used to extract the capacitance values of the HCC and NLC and test the CSM's performance at different distances from the analyte. The PRC's ability to detect the extracted capacitance values of the HCC and NLC is evaluated using Virtuoso Analog Design Environment. A novel algorithm is developed to animate and predict the location and shape of the tested biological cell depending on CSM's capacitance readings simultaneously using MATLAB R2022a script. The results of both models, the measured capacitance from CSM and the correlated frequency from the readout circuit, show the biochip's ability to characterize and distinguish between HCC and NLC.

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Section: Methodsmentioning

confidence: 97%

CMOS based capacitive sensor matrix for characterizing and tracking of biological cells

Abdelbaset

El-Sehrawy

Morsy

et al. 2022

Sci Rep

Self Cite

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show abstract

“…impedance, capacitance, permittivity, and conductivity) of the tested sample using a MATLAB algorithm that solves a set of equations (explained below) based on the measured amplitude of the other electrode of the dual spiral microelectrode through the readout circuit and the phase shift between the input (applied)/the output (measured) signals. However, these electrical aspects are preferred to achieve the best performance of the proposed sensor without using a passivation layer which affect the measuring of the dielectric properties of the tested samples as mentioned in previous work 17,19 .…”

Section: Methodsmentioning

confidence: 99%

A new label free spiral sensor using impedance spectroscopy to characterize hepatocellular carcinoma in tissue and serum samples

Abdelbaset,

Shawky,

Abdullah

et al. 2024

Sci Rep

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Hepatocellular carcinoma (HCC) stands as the most prevalent form of primary liver cancer, predominantly affecting patients with chronic liver diseases such as hepatitis B or C-induced cirrhosis. Diagnosis typically involves blood tests (assessing liver functions and HCC biomarkers), imaging procedures such as Computed Tomography (CT) and Magnetic Resonance Imaging (MRI), and liver biopsies requiring the removal of liver tissue for laboratory analysis. However, these diagnostic methods either entail lengthy lab processes, require expensive imaging equipment, or involve invasive techniques like liver biopsies. Hence, there exists a crucial need for rapid, cost-effective, and noninvasive techniques to characterize HCC, whether in serum or tissue samples. In this study, we developed a spiral sensor implemented on a printed circuit board (PCB) technology that utilizes impedance spectroscopy and applied it to 24 tissues and sera samples as proof of concept. This newly devised circuit has successfully characterized HCC and normal tissue and serum samples. Utilizing the distinct dielectric properties between HCC cells and serum samples versus the normal samples across a specific frequency range, the differentiation between normal and HCC samples is achieved. Moreover, the sensor effectively characterizes two HCC grades and distinguishes cirrhotic/non-cirrhotic samples from tissue specimens. In addition, the sensor distinguishes cirrhotic/non-cirrhotic samples from serum specimens. This pioneering study introduces Electrical Impedance Spectroscopy (EIS) spiral sensor for diagnosing HCC and liver cirrhosis in clinical serum—an innovative, low-cost, rapid (< 2 min), and precise PCB-based technology without elaborate sample preparation, offering a novel non-labeled screening approach for disease staging and liver conditions.

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Dielectrophoresis: Measurement technologies and auxiliary sensing applications

Hu,

Ji,

Chen

et al. 2024

Electrophoresis

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Dielectrophoresis (DEP), which arises from the interaction between dielectric particles and an aqueous solution in a nonuniform electric field, contributes to the manipulation of nano and microparticles in many fields, including colloid physics, analytical chemistry, molecular biology, clinical medicine, and pharmaceutics. The measurement of the DEP force could provide a more complete solution for verifying current classical DEP theories. This review reports various imaging, fluidic, optical, and mechanical approaches for measuring the DEP forces at different amplitudes and frequencies. The integration of DEP technology into sensors enables fast response, high sensitivity, precise discrimination, and label‐free detection of proteins, bacteria, colloidal particles, and cells. Therefore, this review provides an in‐depth overview of DEP‐based fabrication and measurements. Depending on the measurement requirements, DEP manipulation can be classified into assistance and integration approaches to improve sensor performance. To this end, an overview is dedicated to developing the concept of trapping‐on‐sensing, improving its structure and performance, and realizing fully DEP‐assisted lab‐on‐a‐chip systems.

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Detection of foodborne pathogens using novel vertical capacitive sensors

Cited by 6 publications

References 28 publications

CMOS based capacitive sensor matrix for characterizing and tracking of biological cells

CMOS based capacitive sensor matrix for characterizing and tracking of biological cells

A new label free spiral sensor using impedance spectroscopy to characterize hepatocellular carcinoma in tissue and serum samples

Dielectrophoresis: Measurement technologies and auxiliary sensing applications

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