Detection of food-born allergens with aptamer-based biosensors

Khedri, Mostafa; Ramezani, Mohammad; Rafatpanah, Houshang; Abnous, Khalil

doi:10.1016/j.trac.2018.04.001

Cited by 48 publications

(13 citation statements)

References 70 publications

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“…Aptamers can fold into various stable secondary structures (stem, bugle, loop, and G‑quadruplex) and form unique three‐dimensional structures with target allergens through the pairing of complementary bases (Yan et al., 2019) and Electrostatic and hydrogen bonding. Currently, aptamers attracted a great attention and several aptasensing detection strategies have been investigated for food allergen detection because of their small size, low cost, and ease of manufacturing (Khedri, Ramezani, Rafatpanah, & Abnous, 2018). Aptamers are designed as probes in fluorescence sensors, while QDs and nanomagnetic bead are usually employed to embellish the probe.…”

Section: Fluorescence Aptamer Sensorsmentioning

confidence: 99%

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Qian

Zhou

et al. 2020

Comp Rev Food Sci Food Safe

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Food allergies are global health issue with an increasing prevalence that affect food safety; hence, food allergen detection, labeling, and management are considered to be important priorities in the food industry. In this critical review, we provide a comprehensive overview of several fluorescence-based platforms based on different biorecognition ligands, such as antibodies, DNA, aptamers, and cells, for food allergen quantification. Traditional analytical methods are generally unsuitable for food manufacturers to accomplish the real-time identification of food allergens in food products. Therefore, it is important to develop simple, rapid, inexpensive, accurate, and sensitive methods to improve user accessibility. A fluorescence-based quantitative platform provides an excellent detection platform for food allergens because of its high sensitivity. This review summarizes the traditional antibody-based fluorescent techniques for food allergen detection, such as the time-resolved fluoroimmunoassay , immunofluorescence imaging, fluorescence enzyme-linked immune sorbent assay, flow injection fluoroimmunoassay, and fluorescence immunosensors. However, these methods suffer from disadvantages such as the significant rate of false-positive and false-negative results due to antibody cross-reactivity with nontarget food components in the complex food matrix and epitope degradation during food processing. Hence, different types of fluorescence-based immunoassays are suitable for standardization and quantification of allergens in fresh foods. In addition, we summarize new fluorescence-based quantitative platforms, including fluorescence genosensors, fluorescence cell sensors, and fluorescence aptamer sensors. With the advantages of high sensitivity and simple operation, fluorescence biosensors will have great potential in the future and could provide portable methods for multiallergen real-time detection in complex food systems.

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Section: Fluorescence Aptamer Sensorsmentioning

confidence: 99%

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Qian

Zhou

et al. 2020

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

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“…The binding event leads to change of electrical or optical analytical signal at transducer surface and therefore could be easily monitored. Nowadays, aptamers are replacing other biorecognition elements, especially antibodies with similar functionality due to enormous benefits in terms of more stability, easy and cheaper production, lasting longer, and availability of more modification choices [113][114][115][116][117]. Although the use of antibodies as biological reagents is very common, there, however, are several issues associated with antibodies such as ethical issues in their production inside animals, time-consuming process, and high-cost manufacturing.…”

Section: Aptasensorsmentioning

confidence: 99%

“…Over the past few decades, the significant work can be seen on production of aptamers against various targets via systematic evolution of ligands by exponential enrichment (SELEX) [30,114]. Especially for milk allergens, silver decahedral nanoparticles and fluorescence activated cell sorting (AgNPsFACS)-based SELEX has been reported for highly sensitive and specific screening of aptamers against respective milk allergens [119].…”

Section: Aptasensorsmentioning

confidence: 99%

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Nehra

Lettieri

Dilbaghi

et al. 2019

Sensors

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Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins’ presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

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“…The reactions can be caused when even little quantities of food are consumed, usually between 10 and 100 mg [4,8]. It is known that this kind of hypersensitivity is having an impact especially in developed countries: between 1% and 3% of adults and between 3% and 8% of children are affected all over the world [9,10]. Symptoms vary from person to person, but can involve, for example, digestive disorders, circulatory or respiratory symptoms or skin irritations, among others.…”

Section: Introductionmentioning

confidence: 99%

Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes

Amor-Gutiérrez

Selvolini

Fernández‐Abedul

et al. 2020

Sensors

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Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because of this, the development of point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become of outstanding importance. According to this, electrochemical aptasensors have been demonstrated as useful tools to quantify a broad variety of targets. In this work, we develop a simple methodology for the determination of β-lactoglobulin (β-LG) in food samples using a folding-based electrochemical aptasensor built on poly-L-lysine modified graphite screen-printed electrodes (GSPEs) and an anti-β-lactoglobulin aptamer tagged with methylene blue (MB). This aptamer changes its conformation when the sample contains β-LG, and due to this, the spacing between MB and the electrode surface (and therefore the electron transfer efficiency) also changes. The response of this biosensor was linear for concentrations of β-LG within the range 0.1-10 ng·mL −1 , with a limit of detection of 0.09 ng·mL −1 . The biosensor was satisfactorily employed for the determination of spiked β-LG in real food samples.incidence of the most popular food allergies throughout Europe [13] revealed that allergy to cow's milk is, nowadays, the most frequent food allergy, especially in early-age children, implying a percentage of around 6% in the youngest population [14,15]. Immunoglobulin E (IgE) normally mediates cow's milk allergy, and the symptoms usually occur within 2 h after milk intake [16]. In whey from cow's milk, one of the most important proteins considered to be an allergen is β-lactoglobulin (β-LG), which is classified within the lipocalins, a group of binding proteins with small ligands [17]. Its quaternary structure, identified in 1997 using X-Ray diffraction [18], is dependent on the pH. For example, at pH values between 5.2 and 7.0, it is a dimer, and its molecular weight (MW) is around 37 kDa. However, at pH values over 8.0, it exists as a monomer, and its MW is approximately 18 kDa [19]. This protein has a great potential as milk allergen because it is remarkably stable and it is not present in human milk [20], and, apart from that, it is regularly used as an additive in lots of food products [21].The development of analytical approaches used to detect and quantify food allergens has become increasingly significant [22] because labeling in food is crucial for the consumers, in order to prevent major health problems. The analysis of allergens in food samples [23] is usually carried out by immunoassay-based approaches, such as enzyme-linked immunosorbent assays (ELISAs) [11,14,24] or lateral-flow immunoassays (LFIAs) [25,26], as well as DNA-based methods, which detect the genes encoding for the proteins using amplifying methods, for instance, the polymerase chain reaction (PCR) [27,28]. Proteomic tests have been also used for the determination of allergens [29]. Although these methods are very sensitive, they are time-consuming and the instrumenta...

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Detection of food-born allergens with aptamer-based biosensors

Cited by 48 publications

References 70 publications

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes

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