Detection of florfenicol resistance genes in Riemerella anatipestifer isolated from ducks and geese

Chen, Yen-Ping; Lee, Shu-Hwae; Chou, Chung‐Hsi; Tsai, Hsiang‐Jung

doi:10.1016/j.vetmic.2011.07.012

Cited by 56 publications

(34 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An apparent exception to this general trend appears to be the duck pathogen R. anatipestifer, 431 particularly isolates that are circulating in farmed ducks in China (Chen et al 2012;Sun et al 2012). veterinary pathogens is required to inform robust risk assessments in this highly important area.…”

Section: Mof25p and Bgarf1 Respectively 316mentioning

confidence: 99%

Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?

Verner–Jeffreys

Brazier

Perez

et al. 2017

FEMS Microbiology Ecology

View full text Add to dashboard Cite

22Bacteria from the genus Flavobacteriaceae often show low susceptibility to antibiotics. With the 23 exception of two Chryseobacterium spp. isolates that were positive for the florfenicol resistance 24 gene floR, no clinical resistance genes were identified by microarray in 36 Flavobacteriaceae isolates 25 from salmonid fish that could grow in ≥ 4 mg/L florfenicol. Whole genome sequence analysis of the 26 floR positive isolates revealed the presence of a region that contained the antimicrobial resistance 27 (AMR) genes floR, a tet(X) tetracycline resistance gene, a streptothricin resistance gene and a 28 chloramphenicol acetyltransferase gene. In silico analysis of 377 published genomes for 29Flavobacteriaceae isolates from a range of sources, confirmed that well-characterized resistance 30 gene cassettes were not widely distributed in bacteria from this group. Efflux pump-mediated 31 decreased susceptibility to a range of antimicrobials was confirmed in both floR positive isolates 32 using an efflux pump inhibitor (phenylalanine-arginine β-naphthylamide) assay. The floR isolates 33 possessed putative virulence factors, including production of siderophores and, haemolysins, and 34 were mildly pathogenic in rainbow trout. Results support the suggestion that, despite the detection 35 of floR, susceptibility to antimicrobials in Flavobacteriaceae is mostly mediated via intrinsic 36 mechanisms rather than the horizontally acquired resistance genes more normally associated with 37Gram-negative bacterial pathogens such as Enterobacteriaceae. 38 39

show abstract

Section: Mof25p and Bgarf1 Respectively 316mentioning

confidence: 99%

Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?

Verner–Jeffreys

Brazier

Perez

et al. 2017

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…Various antibiotics are currently used to prevent and control R. anatipestifer infection in ducks, but they accelerate the emergence of drug-resistant strains (6,7).The resistance of R. anatipestifer to many antibiotics has increased greatly, and antibiotic residues have been detected in duck-related products (3). Therefore, immunization should be the ideal way to control the disease.…”

mentioning

confidence: 99%

Development and Evaluation of a Trivalent Riemerella anatipestifer-Inactivated Vaccine

Hai-wen¹,

Wang²,

Ding³

et al. 2013

Clin. Vaccine Immunol.

View full text Add to dashboard Cite

b Riemerella anatipestifer infections cause major economic losses in the duck industry. In this study, a trivalent inactivated vaccine of R. anatipestifer, including strains CH3 (serotype 1), NJ3 (serotype 2), and HXb2 (serotype 10), was developed. Animal experiments showed that the ducks that received two immunizations with the vaccine were 100% protected from challenge with strains from any of the three serotypes (1, 2, or 10). No death or clinical signs of diarrhea, tremors, or limb swelling were shown in the protected ducks. Also, no R. anatipestifer bacteria were isolated from the livers or brains of the protected ducks. Furthermore, no histopathological changes were observed in the liver, spleen, or brain samples from the protected ducks during histological examination. The ducks that received two immunizations with the vaccine generated high antibody titers of 1:3,200 to 1:6,400 against the three serotypes of strains. The vaccine significantly enhanced the production of gamma interferon (IFN-␥) and interleukin 2 (IL-2) after one immunization and enhanced the production of IL-4 and IL-10 after two immunizations. In addition, real-time PCR indicated that the expression of major histocompatibility complex I (MHC-I), as well as that of CD40 and CD154 molecules, was significantly increased after one immunization, and the expressions of both MHC-I and MHC-II molecules were increased after two immunizations. Our study indicates that the vaccine can induce both humoral and cellular immunities in ducks and offer effective protection against R. anatipestifer infection.

show abstract

“…Various antibiotics are currently used to prevent and to control R. anatipestifer infections in ducks, but the increasing prevalence of drug-resistant strains is posing a serious challenge for antibiotic treatment (29,30). Therefore, immunization should be the ideal way to prevent ducks from being infected with R. anatipestifer.…”

Section: Discussionmentioning

confidence: 99%

The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor

Wang

Liu

Dou

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Riemerella anatipestifer is a major bacterial pathogen that causes septicemic and exudative diseases in domestic ducks. In our previous study, we found that deletion of the AS87_01735 gene significantly decreased the bacterial virulence of R. anatipestifer strain Yb2 (mutant RA625). The AS87_01735 gene was predicted to encode a nicotinamidase (PncA), a key enzyme that catalyzes the conversion of nicotinamide to nicotinic acid, which is an important reaction in the NAD ؉ salvage pathway. In this study, the AS87_01735 gene was expressed and identified as the PncA-encoding gene, using an enzymatic assay. Western blot analysis demonstrated that R. anatipestifer PncA was localized to the cytoplasm. The mutant strain RA625 (named Yb2⌬pncA in this study) showed a similar growth rate but decreased NAD ؉ quantities in both the exponential and stationary phases in tryptic soy broth culture, compared with the wild-type strain Yb2. In addition, Yb2⌬pncA-infected ducks showed much lower bacterial loads in their blood, and no visible histological changes were observed in the heart, liver, and spleen. Furthermore, Yb2⌬pncA immunization of ducks conferred effective protection against challenge with the virulent wild-type strain Yb2. Our results suggest that the R. anatipestifer AS87_01735 gene encodes PncA, which is an important virulence factor, and that the Yb2⌬pncA mutant can be used as a novel live vaccine candidate. IMPORTANCERiemerella anatipestifer is reported worldwide as a cause of septicemic and exudative diseases of domestic ducks. The pncA gene encodes a nicotinamidase (PncA), a key enzyme that catalyzes the conversion of nicotinamide to nicotinic acid, which is an important reaction in the NAD ؉ salvage pathway. In this study, we identified and characterized the pncA-homologous gene AS87_01735 in R. anatipestifer strain Yb2. R. anatipestifer PncA is a cytoplasmic protein that possesses similar PncA activity, compared with other organisms. Generation of the pncA mutant Yb2⌬pncA led to a decrease in the NAD ؉ content, which was associated with decreased capacity for invasion and attenuated virulence in ducks. Furthermore, Yb2⌬pncA immunization of ducks conferred effective protection against challenge with the virulent wild-type strain Yb2. Altogether, these results suggest that PncA contributes to the virulence of R. anatipestifer and that the Yb2⌬pncA mutant can be used as a novel live vaccine candidate.

show abstract

Detection of florfenicol resistance genes in Riemerella anatipestifer isolated from ducks and geese

Cited by 56 publications

References 18 publications

Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?

Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?

Development and Evaluation of a Trivalent Riemerella anatipestifer-Inactivated Vaccine

The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor

Contact Info

Product

Resources

About