Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia

Saïd, Lamjed Ben; Jouini, Ahlem; Klibi, Naouel; Dziri, Raoudha; Alonso, Carla Andrea; Boudabous, Abdellatif; Slama, Karim Ben; Torres, Carmén

doi:10.1016/j.ijfoodmicro.2015.02.023

Cited by 109 publications

(72 citation statements)

References 38 publications

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“…To date, very few studies have performed an in-depth analysis of mobile elements associated with clinically important bla genes in soil ecosystems. Ultimately, both our study and Ben Said and colleagues (2015) study reflect that the bla-harbouring isolates detected share their molecular background with isolates from human and/or animal sources, pointing to contamination of agricultural soils (Ben Said et al, 2015).…”

Section: Discussionsupporting

confidence: 71%

Influence of agricultural practice on mobile bla genes: IncI1‐bearing CTX‐M, SHV, CMY and TEM in Escherichia coli from intensive farming soils

Jones-Dias

Manageiro

Caniça

2015

Environmental Microbiology

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Many calls have been made to address antibiotic resistance in an environmental perspective. With this study, we showed the widespread presence of high-level antibiotic resistant isolates on a collection of non-susceptible Gram-negative bacteria (n = 232) recovered from soils. Bacteria were selected using amoxicillin, cefotaxime and imipenem, from sites representing different agricultural practices (extensive, intensive and organic). Striking levels of non-susceptibility were noticed in intensive soils for norfloxacin (74%), streptomycin (50.7%) and tetracycline (46.6%); indeed, the exposure to intensive agricultural practices constituted a risk factor for non-susceptibility to many antibiotics, multidrug resistance and production of extended-spectrum β-lactamases (ESBL). Analyses of non-susceptibility highlighted that environmental and clinical bacteria from the same species might not share the same intrinsic resistance patterns, raising concerns for therapy choices in environment-borne infections. The multiple sequence-type IncI1-driven spread of penicillinases (blaTEM-1, blaTEM-135), ESBL (blaSHV-12 and blaCTX-M-1) and plasmid-mediated AmpC β-lactamases (blaCMY-2), produced by isolates that share their molecular features with isolates from humans and animals, suggests contamination of agricultural soils. This is also the first appearance of IncI1/ST28-harbouring blaCTX-M-1, which should be monitored to prevent their establishment as successfully dispersed plasmids. This research may help disclose paths of contamination by mobile antibiotic resistance determinants and the risks for their dissemination.

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Section: Discussionsupporting

confidence: 71%

Influence of agricultural practice on mobile bla genes: IncI1‐bearing CTX‐M, SHV, CMY and TEM in Escherichia coli from intensive farming soils

Jones-Dias

Manageiro

Caniça

2015

Environmental Microbiology

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“…Both ESBL-EC and ESBL-KP infections and colonization have been associated with each of these sources. 19–28 …”

Section: Discussionmentioning

confidence: 99%

Increasing Incidence of Extended-Spectrum β-Lactamase-ProducingEscherichia coliin Community Hospitals throughout the Southeastern United States

Thaden

Fowler

Sexton

et al. 2015

Infect. Control Hosp. Epidemiol.

115

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OBJECTIVE To describe the epidemiology of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) and Klebsiella pneumoniae (ESBL-KP) infections DESIGN Retrospective cohort SETTING Inpatient care at community hospitals PATIENTS All patients with ESBL-EC or ESBL-KP infections METHODS ESBL-EC and ESBL-KP infections from 26 community hospitals were prospectively entered into a centralized database from January 2009 to December 2014. RESULTS A total of 925 infections caused by ESBL-EC (10.5 infections per 100,000 patient days) and 463 infections caused by ESBL-KP (5.3 infections per 100,000 patient days) were identified during 8,791,243 patient days of surveillance. The incidence of ESBL-EC infections increased from 5.28 to 10.5 patients per 100,000 patient days during the study period (P =.006). The number of community hospitals with ESBL-EC infections increased from 17 (65%) in 2009 to 20 (77%) in 2014. The median ESBL-EC infection rates among individual hospitals with ≥1 ESBL-EC infection increased from 11.1 infections/100,000 patient days (range, 2.2–33.9 days) in 2009 to 22.1 infections per 100,000 patient days (range, 0.66–134 days) in 2014 (P =.05). The incidence of ESBL-KP infections remained constant over the study period (P = .14). Community-associated and healthcare-associated ESBL-EC infections trended upward (P =.006 and P = .02, respectively), while hospital-onset infections remained stable (P = .07). ESBL-EC infections were more common in females (54% vs 44%, P < .001) and Caucasians (50% vs 40%, P < .0001), and were more likely to be isolated from the urinary tract (61% vs 52%, P < .0001) than ESBL-KP infections. CONCLUSIONS The incidence of ESBL-EC infection has increased in community hospitals throughout the southeastern United States, while the incidence of ESBL-KP infection has remained stable. Community- and healthcare-associated ESBL-EC infections are driving the upward trend.

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“…The β-lactamase enzymes are derived from mutations in temoneira (TEM), sulfhydryl variable (SHV), and CTX-M genes located on bacterial plasmids or chromosomes (Ben Said et al, 2015;Legese et al, 2017;Adelowo et al, 2018). These genes can easily be horizontally transferred from one bacterial strain to another including across bacterial species (Lavilla et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

et al. 2020

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Extended-spectrum β-lactamase (ESBL)-producing bacteria constitute an emerging global health issue with food products being vehicles of transmission and the aquatic environments serving as potential reservoirs. This study aimed to characterize ESBLproducing Escherichia coli in Nile perch and water from Lake Victoria in Tanzania. A total of 180 samples of Nile perch and 60 water samples were screened for ESBL-producing E. coli on MacConkey agar supplemented with 2 µg/ml of cefotaxime and confirmed by bla CTX−M and bla TEM PCR. Antimicrobial resistance was determined by the disk diffusion method, and the ESBL-producing isolates were whole genome sequencing (WGS). ESBL-producing E. coli were detected in eight of the 180 analyzed Nile perch samples, and only one water sample was positive (1.7%, n = 60). Isolates were resistant to sulfamethoxazole-trimethoprim (100%), ampicillin/cloxacillin (100%), erythromycin 72.7% (8/11), tetracycline 90.9% (10/11), and nalidixic acid 63.6% (7/11). This mostly corroborates the resistance genes that they carried for sulfonamides (sul1 and sul2), trimethoprim (dfrA and dfrB), aminoglycosides [aac(3)-IId, strA, and strB], tetracycline [tet(B) and tet(D)], and fluoroquinolones (qepA4). They harbored plasmid replicon types IncF, IncX, IncQ, and Col and carried bla CTX−M−15 and bla TEM−1B genes generally found on the same contigs as the IncF plasmid replicon. Although epidemiologically unrelated, the strains formed three separate sequence type-phylogroup-serotypespecific clusters: C1, C2, and C3. Cluster C1 included five strains (3 to 13 SNPs) belonging to ST167, phylogroup A, and serotype O9:H21; the two C2 strains (11 SNPs) belong to ST156, phylogroup B1, and serotype ONT:H28; and C3 was made up of four strains (SNPs ranged from 4 to 17) of ST636, phylogroup B2, and serotype O45:H7. The common virulence gene gad was reported in all strains. In addition, strains in C2 and C3 possessed iss, lpfA, and nfaE virulence genes, and the vat gene was found only in C3. The present study reports the occurrence of multidrug-resistant ESBL-producing Frontiers in Microbiology | www.frontiersin.org February 2020 | Volume 11 | Article 108 Baniga et al.Genomics of ESBL-E. coli in Lake Victoria E. coli carrying plasmid-mediated ESBL genes in offshore water and Nile perch in Lake Victoria. Strains formed three clonal clusters of unknown origin. This study reveals that the Lake may serve as reservoir for ESBL-producing bacteria that can be transmitted by fish as a food chain hazard of One-Health concern.

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Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia

Cited by 109 publications

References 38 publications

Influence of agricultural practice on mobile bla genes: IncI1‐bearing CTX‐M, SHV, CMY and TEM in Escherichia coli from intensive farming soils

Influence of agricultural practice on mobile bla genes: IncI1‐bearing CTX‐M, SHV, CMY and TEM in Escherichia coli from intensive farming soils

Increasing Incidence of Extended-Spectrum β-Lactamase-ProducingEscherichia coliin Community Hospitals throughout the Southeastern United States

Genome-Based Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Aquatic Environment and Nile Perch (Lates niloticus) of Lake Victoria, Tanzania

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