Detection of exosomes by ZnO nanowires coated three-dimensional scaffold chip device

Chen, Zhe; Cheng, Shi‐Bo; Cao, Pan; Qiu, Quan-Fa; Chen, Yan; Xie, Min; Yu, Xu; Huang, Wei‐Hua

doi:10.1016/j.bios.2018.09.033

Cited by 106 publications

(66 citation statements)

References 39 publications

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“…[240] In this regard, different technologies can be implemented to bridge this gap, including ultracentrifugation/gradient techniques, sizeexclusion chromatography, ultrafiltration, microfluidics chips, ultimately improving the quality (i.e., size, density, potential, and biochemical composition), and the yield of EVs. In particular, novel technologies based on innovative 1D [241][242][243][244] and 2D materials [245][246][247] may largely facilitate the capture, detection, quantification and quality control of purified EVs, helping to gain a larger foothold in the healthcare and nanomedicine market.…”

Section: Productionmentioning

confidence: 99%

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Leggio

Arrabito

Ferrara

et al. 2020

Adv Healthcare Materials

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Naturally occurring extracellular vesicles and artificially made vesicles represent important tools in nanomedicine for the efficient delivery of biomolecules and drugs. Since its first appearance in the literature 50 years ago, the research on vesicles is progressing at a fast pace, with the main goal of developing carriers able to protect cargoes from degradation, as well as to deliver them in a time‐ and space‐controlled fashion. While natural occurring vesicles have the advantage of being fully compatible with their host, artificial vesicles can be easily synthetized and functionalized according to the target to reach. Research is striving to merge the advantages of natural and artificial vesicles, in order to provide a new generation of highly performing vesicles, which would improve the therapeutic index of transported molecules. This progress report summarizes current manufacturing techniques used to produce both natural and artificial vesicles, exploring the promises and pitfalls of the different production processes. Finally, pros and cons of natural versus artificial vesicles are discussed and compared, with special regard toward the current applications of both kinds of vesicles in the healthcare field.

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Section: Productionmentioning

confidence: 99%

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Leggio

Arrabito

Ferrara

et al. 2020

Adv Healthcare Materials

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“…Another microfluidic design, in which there is an integration of several steps, was presented by Chen et al [105]. It consisted of a 3D macroporous chip device with integrated ZnO nanowires to effectively isolate exosomes, as well as quantify them by colorimetric assay ( Figure 4B).…”

Section: Platforms Based On Exosomes Analysismentioning

confidence: 99%

“…It consisted of a 3D macroporous chip device with integrated ZnO nanowires to effectively isolate exosomes, as well as quantify them by colorimetric assay ( Figure 4B). The ZnO nanowires together with the 3D interconnected macropores confer a great surface area as well as chaotic mixing, increasing the efficiency to capture the exosomes [105].…”

Section: Platforms Based On Exosomes Analysismentioning

confidence: 99%

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

Sierra

Marrugo-Ramírez

Rodriguez-Trujíllo

et al. 2020

Sensors

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Cancer represents one of the conditions with the most causes of death worldwide. Common methods for its diagnosis are based on tissue biopsies—the extraction of tissue from the primary tumor, which is used for its histological analysis. However, this technique represents a risk for the patient, along with being expensive and time-consuming and so it cannot be frequently used to follow the progress of the disease. Liquid biopsy is a new cancer diagnostic alternative, which allows the analysis of the molecular information of the solid tumors via a body fluid draw. This fluid-based diagnostic method displays relevant advantages, including its minimal invasiveness, lower risk, use as often as required, it can be analyzed with the use of microfluidic-based platforms with low consumption of reagent, and it does not require specialized personnel and expensive equipment for the diagnosis. In recent years, the integration of sensors in microfluidics lab-on-a-chip devices was performed for liquid biopsies applications, granting significant advantages in the separation and detection of circulating tumor nucleic acids (ctNAs), circulating tumor cells (CTCs) and exosomes. The improvements in isolation and detection technologies offer increasingly sensitive and selective equipment’s, and the integration in microfluidic devices provides a better characterization and analysis of these biomarkers. These fully integrated systems will facilitate the generation of fully automatized platforms at low-cost for compact cancer diagnosis systems at an early stage and for the prediction and prognosis of cancer treatment through the biomarkers for personalized tumor analysis.

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“…Using this nanomaterialmodified microfluidic device, Chen et al successfully isolated a large number of exosomes (2.2 × 10 4 exosomes µL −1 ) from µL-volumes of plasma samples, and highlighted the applicability of the miniaturized platform for clinical sample analysis (whereby number of exosomes remained significantly higher in cancer patients as compared to healthy individuals). [32] More recently, Zhang et al developed a nanoherringbone chip by patterning silica nanoparticles within a microfluidic chip for purification of extremely low number of exosomes from plasma samples. [84] The patterned nanostructures within the microfluidic device facilitated microscale mass transfer, increased micromixing, and permitted easy passing of boundary fluids through the pores of nanoherringbone structures to minimize near-surface hydrodynamic resistance.…”

Section: Cancer-associated Ev Preparationmentioning

confidence: 99%

Section: Na Preparationmentioning

confidence: 99%

The Growing Impact of Micro/Nanomaterial‐Based Systems in Precision Oncology: Translating “Multiomics” Technologies

Wuethrich

Dey

et al. 2020

Adv Funct Materials

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The field of precision oncology is rapidly progressing toward integrated “multiomics” analysis of multiple molecular species (such as DNA, RNA, or proteins) to provide a more complete profile of tumor heterogeneity. Micro/nanomaterial‐based systems, which leverage the unique properties of miniature materials, are currently well positioned to expand beyond rudimentary biomarker detection toward multiomics signature analysis. To enable clinical translation, the rational design and implementation of miniaturized systems should be driven by the unique clinical challenges present at various crucial cancer stages. This review features micro/nanomaterial‐based systems that are robustly tested on real patient samples for molecular biomarker detection at i) initial cancer screening and/or diagnosis, ii) cancer prognosis and risk stratification, and iii) longitudinal treatment/recurrence monitoring. Furthermore, this review discusses the use of micro/nanomaterials to facilitate sample preparation for different molecular biomarker species. Finally, this review deliberates on the recent paradigm shift of micro/nanomaterial‐based system innovation toward integrated multiomics cancer signature analysis and puts forth insights and perspectives on existing challenges. It is anticipated that this review could stimulate the propagation of new concepts and approaches to kick‐start a new generation of clinically translational technologies that capitalize on multiomics cancer signatures.

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Detection of exosomes by ZnO nanowires coated three-dimensional scaffold chip device

Cited by 106 publications

References 39 publications

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Mastering the Tools: Natural versus Artificial Vesicles in Nanomedicine

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

The Growing Impact of Micro/Nanomaterial‐Based Systems in Precision Oncology: Translating “Multiomics” Technologies

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