Detection of Escherichia coli in a cattle manure composting process by selective cultivation and colony polymerase chain reaction

Asano, Ryoki; Kubori, Kiyohide; Ozutsumi, Yuhei; Yamamoto, Nozomi; Otrawa, Kenichi; Nakai, Yutaka

doi:10.1080/03601234.2011.534972

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Meanwhile, colony PCR has been widely used in the identi cation of positive clones (Woodman et al, 2016).Colony PCR directly uses the DNA exposed after thermal hydrolysis of bacterial bodies as a template for PCR ampli cation, which saves a series of complex processes such as extraction of bacterial body DNA (Asano et al, 2011). Compared with the traditional method of extraction of bacterial body DNA and then PCR ampli cation, it is more time-saving and labor-saving and suitable for rapid batch identi cation of bacterial strains (Kong et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Diversity Analysis of Rhizosphere Soil Microbial Population Structure and Screening and Identification of Dominant Salt-Tolerant Strains of JunCao"Oasis No. 1"

Xiao

Zhifang

2022

Preprint

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Purpose: As a newly cultivated grass species widely used in saline-alkali soil treatment, Juncao"Oasis No.1" can effectively enhance the microbial population abundance in saline-alkali soil. It has a good effect on improving saline-alkali soil.Methods: In order to further study the microbial community in the rhizosphere soil of Juncao"Oasis No.1" , the dominant strains with important ecological value were screened out. In this paper, soil samples were collected from saline-alkali soil planted with Juncao"Oasis No. 1" and unplanted Juncao"Oasis No. 1", and the microbial populations were sequenced by 16SrDNA high-throughput sequencing, in order to identify the difference of microbial population, the dominant bacteria in rhizosphere soil of Juncao"Oasis No. 1" was selected, and the dominant bacteria with important ecological value were isolated and cultured and identified by Colony PCR.Results: The results showed that Pseudomonas fluorescens and Bacillus thuringiensis. were the dominant strains with important ecological value in rhizosphere soil of Juncao"Oasis No.1" . After isolation and culture, Colony PCR identification showed that the dominant strain was successfully isolated and cultured.Conclusions: In this paper, the dominant strains with important ecological value in rhizosphere soil of Juncao" Oasis No. 1" were screened and cultured, which provided theoretical basis for the improvement of saline-alkali soil by using Juncao"Oasis No. 1", it is of great guiding significance.

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Section: Introductionmentioning

confidence: 99%

Diversity Analysis of Rhizosphere Soil Microbial Population Structure and Screening and Identification of Dominant Salt-Tolerant Strains of JunCao"Oasis No. 1"

Xiao

Zhifang

2022

Preprint

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“…A number of previous studies have shown that E. coli can survive for up to several months in experimentally inoculated animal feces (Kudva et al, 1998;Lung et al, 2001;Asano et al, 2011). The disinfection of manure during composting is based on the time-temperature relationship that kills the pathogens plus the action of antagonistic microorganisms during this process (Himathongkham and Rieman, 1999;Turner, 2002;Larney et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Fate of non O157 Shigatoxigenic Escherichia coli in ovine manure composting

Marin

Maluta

Borges

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Livestock manure may contain pathogenic microorganisms which pose a risk to the health of animal or humans if the manure is not adequately treated or disposed of. To determine the fate of Shiga toxigenic Escherichia coli (STEC) non O157 in composted manure from naturally colonized sheep, fresh manure was obtained from animals carrying bacterial cells with stx1/ stx2 genes. Two composting systems were used, aerated and non-aerated, and the experiments were done in Dracena city, São Paulo State. Every week, for seven weeks, one manure sample from six different points in both systems was collected and cultured to determine the presence of E. coli, the presence of the virulence genes in the cells, and also the susceptibility to 10 antimicrobial drugs. The temperature was verified at each sampling. STEC non-O157 survived for 49 days in both composting systems. E. coli non-STEC showing a high degree of antibiotic resistance was recovered all long the composting period. No relationship was established between the presence of virulence genes and antibiotic resistance. The presence of virulence genes and multiple antibiotic resistances in E. coli implicates a potential risk for these genes spread in the human food chain, which is a reason for concern.Keywords: bacterial elimination, VTEC, environment, compost, virulence gene RESUMO

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Effects of humic acids derived from lignite and cattle manure on antioxidant enzymatic activities of barley root

Nagasawa

Wang

Nishiya

et al. 2015

Journal of Environmental Science and Health, Part B

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To investigate the effects of humic acids (HAs) on the ability of plants to defend themselves against oxidative stress, barley was hydroponically cultured in the absence and presence of HAs, and the antioxidant enzymatic activities (catalase, superoxide dismutase, ascorbate and glutathione peroxidases) of root tissue were evaluated. Auxin-like structures in HAs, which were extracted from an oxidation product of lignite (LHA) and compost derived from cattle manure (CHA), were identified by pyrolysis-gas chromatography/mass spectrometry (GC/MS) with tetramethylammonium hydroxide. The LHA, which had the lower molecular weight, was more effective in promoting the growth of barley root than CHA. However, the amounts of auxin-like structures in the CHA were much higher than those for LHA. The antioxidant enzymatic activities were initially decreased in the presence of LHA and CHA at the first day after refreshing the culture solution, but were significantly increased on the second day. The CHA sample, which contained relatively high levels of phenolic acids that contained auxin-like structures, was effective in increasing four types of enzymatic activities, while the activities of catalase and ascorbate peroxidase were increased in the presence of LHA, which contains naphthalene derivatives. These results indicate that using HAs as a supplement can be effective in enhancing antioxidation enzymatic activities, while the appearance of the effects is retarded because of the decomposition and release of auxin-like compounds from HAs by organic acids from the plant roots.

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Detection of Escherichia coli in a cattle manure composting process by selective cultivation and colony polymerase chain reaction

Cited by 3 publications

References 31 publications

Diversity Analysis of Rhizosphere Soil Microbial Population Structure and Screening and Identification of Dominant Salt-Tolerant Strains of JunCao"Oasis No. 1"

Diversity Analysis of Rhizosphere Soil Microbial Population Structure and Screening and Identification of Dominant Salt-Tolerant Strains of JunCao"Oasis No. 1"

Fate of non O157 Shigatoxigenic Escherichia coli in ovine manure composting

Effects of humic acids derived from lignite and cattle manure on antioxidant enzymatic activities of barley root

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