Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal

Abstract

Aim:The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal.Materials and Methods:The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of blaCTX-M, blaTEM, blaSHV, blaVIM, tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance.Results:In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, blaCTX-M was detected in 18 (36%) isolates, and 6 (12%) harbored blaTEM genes in PCR. None of the isolates carried blaSHV genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates.Conclusion:This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India.

Characterization of Escherichia coli Isolated from Bovine Subclinical Mastitis for Virulence Genes, Phylogenetic Groups and ESBL Production

“…Farm animals are considered as established reservoirs for ESBL type Enterobacteriaceae. In India, few investigators have studied the ESBL type E. coli associated with bovine mastitis (Ghatak et al, 2013 andDas et al, 2017). To the best of our knowledge, this is first report from India on detection of E. coli pathotypes, their phylogrouping and ESBL production in the strains from bovine mastitis.…”

“…Present study affirm that cattle are one of the predominant reservoirs of pathogenic E. coli. Isolation of E. coli from cases of bovine mastitis has been reported from India (Das et al, 2017), however, virulent genes and E. coli phylogenetic groups were not detected earlier except few In this regards you may please reply of the reviewer comments mail.…”

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

“…In India CTX-M-15, CTX-M-63, CTX-M, SHV-180, SHV and TEM in E-ESBL from cattle have already been described. Faecal origin and mastitis, being detected in E. coli and K. pneumoniae (Borah et al, 2014;Das et al, 2017;Koovapra et al, 2016). The presence of CTX-M-15 and SHV in E-ESBL in India has been reported in humans (Hawkey, 2008).…”

TESTE DE HODGE MODIFICADO EM ÁGAR CLED PARA TRIAGEM DE Proteus mirabilis PRODUTORES DE CARBAPENEMASE

“…Outros mecanismos podem estar relacionados à resistência observada aos carbapenemas nesses isolados, como a diminuição da permeabilidade da membrana bacteriana ou a coprodução de enzimas (TSAI;WANG;HSUEH, 2015;MOHAMMED et al, 2016;DAS et al, 2017). Esses dados são fundamentais para a escolha apropriada da antibioticoterapia que será utilizada no tratamento de infecções causadas por essas bactérias e para o monitoramento das cepas de Proteus resistentes circulantes (HARADA et al, 2014).…”