Detection of elastin in the human fetal membranes: Proposed molecular basis for elasticity

Hieber, A. David; Martinez, Diana; Motosue, J.; Sandberg, Lawrence B.; Roos, Philip J.; Yu, Shiu Yeh; Csiszár, Katalin; Kagan, Herbert M.; Boyd, Charles D.; Bryant‐Greenwood, Gillian D.

doi:10.1016/s0143-4004(97)80065-3

Cited by 51 publications

(32 citation statements)

References 30 publications

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“…The AM has proper viscoelasticity to be employed in biomedical engineering [10]. Matrix of the AM contains a large amount of elastin and collagen that give it viscoelastic property [64]. All layers of the AM include collagens type I, III, V, and VI [32].…”

Section: Evidencementioning

confidence: 99%

Extract of fetal membrane would inhibit thrombosis and hemolysis

2015

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Section: Evidencementioning

confidence: 99%

Extract of fetal membrane would inhibit thrombosis and hemolysis

2015

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“…It was reported that Young's modulus of the AM is between 2.29 and 3.6 MPa, where this modulus decreases as the fetal is reaching term [85]. Elastin has been proposed to provide the molecular basis for the aforementioned elasticity in the AM [86].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

et al. 2010

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“…An intensity factor was defined for 84 each image as the sum of pixels intensity normalized for its dimensions and grey scale. An FM [34]. Histological sections stained with resorcin fuchsin showed few thin layers of elastin, the 144 most distinct of which is below the epithelium.…”

mentioning

confidence: 96%

Second harmonic generation microscopy of fetal membranes under deformation: Normal and altered morphology

et al. 2013

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INTRODUCTION: Insight into the microstructure of fetal membrane and its response to deformation is important for understanding causes of preterm premature rupture of the membrane. However, the microstructure of fetal membranes under deformation has not been visualized yet. Second harmonic generation microscopy, combined with an in-situ stretching device, can provide this valuable information. METHODS: Eight fetal membranes were marked over the cervix with methylene blue during elective caesarean section. One sample per membrane of reflected tissue, between the placenta and the cervical region, was cyclically stretched with a custom built inflation device. Samples were mounted on an in-situ stretching device and imaged with a multiphoton microscope at different deformation levels. Microstructural parameters such as thickness and collagen orientation were determined. Image entropy was evaluated for the spongy layer. RESULTS: The spongy layer consistently shows an altered collagen structure in the cervical and cycled tissue compared with the reflected membrane, corresponding to a significantly higher image entropy. An increased thickness of collagenous layers was found in cervical and stretched samples in comparison to the reflected tissue. Significant collagen fibre alignment was found to occur already at moderate deformation in all samples. CONCLUSIONS: For the first time, second harmonic generation microscopy has been used to visualize the microstructure of fetal membranes. Repeated mechanical loading was shown to affect the integrity of the amnion-chorion interface which might indicate an increased risk of premature rupture of fetal membrane. Moreover, mechanical loading might contribute to morphological alterations of the fetal membrane over the cervical region. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT Second Harmonic Generation Microscopy of Foetal Membranes under

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Detection of elastin in the human fetal membranes: Proposed molecular basis for elasticity

Cited by 51 publications

References 30 publications

Extract of fetal membrane would inhibit thrombosis and hemolysis

Extract of fetal membrane would inhibit thrombosis and hemolysis

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

Second harmonic generation microscopy of fetal membranes under deformation: Normal and altered morphology

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