Detection of East/Central/South African genotype Chikungunya virus during an outbreak in a southeastern state of Brazil

Lessa-Aquino, Carolina; Trinta, Karen Soares; Pestana, Cristiane Pinheiro; Ribeiro, Marisa; Sucupira, Michel Vergne; Bóia, Márcio Neves; Baptista, P. A.; Cunha, Rivaldo Venâncio da; Medeiros, Marco Alberto

doi:10.1017/s0950268818002467

Cited by 11 publications

(9 citation statements)

References 11 publications

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“…aegypti was first documented with natural infection by CHIKV in 2017 in Brazil and the Americas, attributed to the ECSA genotype [5], the same found in the present study. This finding aligns with the widespread prevalence of the ECSA genotype since its introduction in 2014 [45], frequently detected in humans CHIK cases in Brazil [46,47], included those in the state of Minas Gerais [48]. Despite its importance, there are few reports on genomic CHIKV surveillance in mosquitoes from Brazil [6,[12][13][14][49][50][51][52].…”

Section: Discussionsupporting

confidence: 66%

Instagram Helped to Reveal That Aedes aegypti (Diptera: Culicidae) Was the Main Vector of Chikungunya Virus (ECSA-Genotype) and Presented High Frequencies of kdr Mutations during an Explosive Outbreak in Minas Gerais, Brazil, 2023

Almeida-Souza,

Oliveira,

Brito

et al. 2024

Preprint

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The Chikungunya virus (CHIKV) presents global health challenges, with Brazil witnessing out-breaks since its introduction in 2014. In 2023, Minas Gerais (MG) experienced a CHIKV outbreak, prompting entomological investigations to identify vectors, viral lineages, and assess insecticide resistance. This study utilized social media platforms and citizen science concept to conduct a comprehensive entomological survey. Following Instagram posts, residents with CHIKV sus-pected cases were able to schedule mosquito aspirations. During the aspirations, 421 mosquitoes (165 Aedes aegypti and 256 Culex quinquefasciatus) were captured from 40 households in Salinas city (MG) and tested for Dengue, Zika and Chikungunya viruses through RT-qPCR. Twelve out 57 pools (10 of Ae. aegypti and 2 of Cx. quinquefasciatus) tested positive for CHIKV RNA. Viral RNA was also detected in the heads of 9 Ae. aegypti individuals (but not in Cx. quinquefasciatus). Genome sequencing revealed that CHIKV belonged to the East-Central-South-Africa (ECSA) genotype. Additionally, genetic analyses revealed high frequencies of kdr alleles, suggesting resistant to pyrethroids insecticides in this Ae. aegypti population. These findings emphasize the urgent need for innovative vector study and control strategies to combat arbovirus transmission, and inter-disciplinary approaches in public health interventions.

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Section: Discussionsupporting

confidence: 66%

Instagram Helped to Reveal That Aedes aegypti (Diptera: Culicidae) Was the Main Vector of Chikungunya Virus (ECSA-Genotype) and Presented High Frequencies of kdr Mutations during an Explosive Outbreak in Minas Gerais, Brazil, 2023

Almeida-Souza,

Oliveira,

Brito

et al. 2024

Preprint

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“…The CHIKV ECSA genotype was first reported in Brazil in 2014, in Feira de Santana, Bahia, in the Northeast region and, in the following years, spread to other states, being identified in Bahia (2014–2017) [ 9 , 48 , 49 ], Alagoas (2016) [ 46 , 50 ], Piauí (2016–2017) [ 51 ], Sergipe (2016) [ 38 ], and Maranhão (2017) [ 40 ]. It was also identified in the Southeast region, in Rio de Janeiro (2016–2018) [ 52 , 53 ] and Minas Gerais (2017) [ 54 ]. Moreover, it has been suggested that the probable introduction of the ECSA genotype in the North occurred from the Northeast in mid-2015 [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Chikungunya virus Detection in Aedes aegypti and Culex quinquefasciatus during an Outbreak in the Amazon Region

Cruz

Neto

Silva

et al. 2020

Viruses

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Chikungunya virus (CHIKV) was first reported in Brazil in 2014 and, after it spread countrywide, an outbreak of febrile illness with reports of arthralgia happened in the municipality of Xinguara, Pará, Brazil in 2017, indicating the virus’ circulation. Here, we aimed to investigate CHIKV in mosquito vectors collected during an active surveillance of virus isolation in cell culture by using molecular detection and viral genome sequencing. A total of 492 Aedes, Culex and Mansonia mosquitoes were collected and separated in 36 pools according to the species and sex, and 22.2% (8/36) were positive. CHIKV was indentified in pools of Ae. aegypti females (n = 5), an Ae. aegypti male (n = 1) and in Culex quinquefasciatus females (n = 2). However, as the mosquitoes’ whole bodies were macerated and used for detection, one cannot suggest the role of the latter in the viral transmission. Despite this, vector competence studies must be carried out in the different species to investigate long-term adaptations. Viral genome sequencing has characterized the East-Central-South-African (ECSA) genotype in all positive pools analyzed, corroborating previous reports for the Amazon region.

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“…aegypti in Brazil are restricted to few groups. The available data about CHIKV circulation are obtained from the human cases that show, until now, the circulation of the Asian genotype, in the Northern (state of Amapá [15]) and Southeast (Rio de Janeiro [91,92]) regions, and the ECSA genotype, reported in the Northern (Amazônia [93]), Northeast (Alagoas [88,89], Bahia [15,88,90], Paraíba [88], and Sergipe [90]), and Southeast (in Rio de Janeiro [90,94] and Minas Gerais [95]).…”

Section: Discussionmentioning

confidence: 99%

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

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Detection of East/Central/South African genotype Chikungunya virus during an outbreak in a southeastern state of Brazil

Cited by 11 publications

References 11 publications

Instagram Helped to Reveal That Aedes aegypti (Diptera: Culicidae) Was the Main Vector of Chikungunya Virus (ECSA-Genotype) and Presented High Frequencies of kdr Mutations during an Explosive Outbreak in Minas Gerais, Brazil, 2023

Instagram Helped to Reveal That Aedes aegypti (Diptera: Culicidae) Was the Main Vector of Chikungunya Virus (ECSA-Genotype) and Presented High Frequencies of kdr Mutations during an Explosive Outbreak in Minas Gerais, Brazil, 2023

Chikungunya virus Detection in Aedes aegypti and Culex quinquefasciatus during an Outbreak in the Amazon Region

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

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