Detection of dry body fluids by inherent short wavelength UV luminescence: preliminary results

Springer, Eliot; Almog, Joseph; Frank, Arie; Ziv, Z.; Bergman, Peder; Qiang, Wu

doi:10.1016/0379-0738(94)90332-8

Cited by 21 publications

(14 citation statements)

References 2 publications

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“…In none of the cases investigated was this statement relevant under legal or financial aspects. UV fluorescence has been known for detection of biological traces for a long time [6,7,8]. In the present study the use of an alternative light source (ALS, Polilight) has turned out to be a reasonable screening method for the examination of the airbags.…”

Section: Discussionmentioning

confidence: 99%

Airbag contact in traffic accidents: DNA detection to determine the driver identity

Grubwieser

Pavlic

Günther

et al. 2004

International Journal of Legal Medicine

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A total of 34 deployed driver and passenger airbags from altogether 20 vehicles after frontal collisions were investigated. In 80% of the airbags possible biological traces could be located with an alternative light source (ALS, Polilight) at a wavelength of 450-470 nm. These traces were swabbed, a part of them additionally cut and subjected to DNA analysis, which led to comparable SGMplus profiles in about 60%. In the 20% of the airbags on which no possible biological traces could be located, the whole surfaces were swabbed. In these cases subsequent DNA profiling mostly led to non-interpretable results. For the evaluation and interpretation of the data, buccal swab samples provided by drivers and co-drivers were analysed. The results and conclusions from DNA analyses and the declarations from the involved passengers were always concordant. Thus, molecular biological analysis of deployed airbags can help to determine the occupants positions within a vehicle (driver or passenger status) at the time of impact.

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Section: Discussionmentioning

confidence: 99%

Airbag contact in traffic accidents: DNA detection to determine the driver identity

Grubwieser

Pavlic

Günther

et al. 2004

International Journal of Legal Medicine

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“…[8] Researches in the field of biophysics after fluorescent spectroscopy have brought revolution in the field of forensic science. Fluorescence spectroscopy is widely used to analyze structure, dynamics and functional interactions of proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Short UV luminescence using Nd:YAG laser emitting at 266 nm has also been tried in detecting saliva stains invisible to the naked eye in a preliminary study, which has certain disadvantages like risk of burning one’s hand, conjunctivitis and lack of portability. [8] Recent studies done by the same research group concerning the use of mercury xenon lamp and CCD camera for detection of the fluorescence of different body fluids including saliva did not show any clear data. [12]…”

Section: Discussionmentioning

confidence: 99%

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A rapid and noninvasive method to detect dried saliva stains from human skin using fluorescent spectroscopy

Nanda

Ranganathan

Umadevi

et al. 2011

J Oral Maxillofac Pathol

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Objective:Saliva is one of the vital fluids secreted in human beings. Significant amount of saliva is deposited on the skin during biting, sucking or licking, and can act as an important source in forensic evidence. An enzyme, α amylase, gives a characteristic emission spectrum at 345–355 nm when excited at 282 nm and this can be identified by using fluorescent spectroscopy and can help in forensic identification. This study describes a rapid method to detect dried saliva on the human skin by fluorescent spectroscopy.Materials and Methods:This study included 10 volunteers, who deposited their own saliva on skin of their ventral forearm by licking and water on the contralateral arm as control. This study was carried out at Central Leather Research Institute, Chennai.Study design:Ten volunteers deposited their own saliva on skin of their ventral forearm by licking. A control sample of water was deposited at the contralateral arm. Each sample was excited at 282 nm and emission spectrum was recorded.Results:The emission spectra of 10 swab samples taken from dried saliva were characterized at the primary peak of 345 to 355 nm whereas the emission spectrum of water as a control was recorded at 362 nm.Conclusion:The presence of emission spectrum at 345–355 nm with excitation at 282 nm proves to be a strong indicator of saliva deposited on human skin.

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“…Springer et al (136,137) have performed the forensic detection of stains of body fluids such as blood, semen, and saliva invisible to the naked eye using short-wavelength luminescence and a CCD camera. They used a frequency-quadrupled Nd:YAG laser emitting at 266 nm or a mercury-xenon lamp under deep-UV region as the excitation source, and the autofluorescence images were recorded due to the presence of amino acids in stains of body fluids.…”

Section: Detection Of Latent Fingerprints Petroleum Accelerants Andmentioning

confidence: 99%

Autofluorescence Detection in Analytical Chemistry and Biochemistry

Seeger

2010

Applied Spectroscopy Reviews

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Label-free detection of molecules and particles based on native fluorescence excited at the ultraviolet (UV) region of the electromagnetic spectrum shows great potential for molecular analysis in life sciences. It offers simple, low-cost, and fast methods for sensitive detection of important biological analytes in nature forms. This article reviews the most significant research on label-free native fluorescence detection in analytical chemistry and biochemistry, with particular focus on the instrumental requirements and applications that cover papers published to the beginning of 2009. Future development directions for label-free optical biosensors based on native fluorescence are discussed.

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Detection of dry body fluids by inherent short wavelength UV luminescence: preliminary results

Cited by 21 publications

References 2 publications

Airbag contact in traffic accidents: DNA detection to determine the driver identity

Airbag contact in traffic accidents: DNA detection to determine the driver identity

A rapid and noninvasive method to detect dried saliva stains from human skin using fluorescent spectroscopy

Autofluorescence Detection in Analytical Chemistry and Biochemistry

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