Objective:Saliva is one of the vital fluids secreted in human beings. Significant amount of saliva is deposited on the skin during biting, sucking or licking, and can act as an important source in forensic evidence. An enzyme, α amylase, gives a characteristic emission spectrum at 345–355 nm when excited at 282 nm and this can be identified by using fluorescent spectroscopy and can help in forensic identification. This study describes a rapid method to detect dried saliva on the human skin by fluorescent spectroscopy.Materials and Methods:This study included 10 volunteers, who deposited their own saliva on skin of their ventral forearm by licking and water on the contralateral arm as control. This study was carried out at Central Leather Research Institute, Chennai.Study design:Ten volunteers deposited their own saliva on skin of their ventral forearm by licking. A control sample of water was deposited at the contralateral arm. Each sample was excited at 282 nm and emission spectrum was recorded.Results:The emission spectra of 10 swab samples taken from dried saliva were characterized at the primary peak of 345 to 355 nm whereas the emission spectrum of water as a control was recorded at 362 nm.Conclusion:The presence of emission spectrum at 345–355 nm with excitation at 282 nm proves to be a strong indicator of saliva deposited on human skin.
Aim:Sister chromatid exchange (SCE) test is a sensitive, biomarker of genotoxic substances. The present study was conducted to observe the frequency of SCEs in peripheral blood lymphocytes of 30 males with and without the habit of cigarette smoking and alcohol consumption.Materials and Methods:Subjects for this study were males aged between 25-50 years and were selected from the students, employees and the patients attending the outpatient department of Ragas dental college and Hospital, Chennai.Results:Controls, smokers, and smokers with alcohol habit were divided into two age groups as ≤30 years and ≥30 years. In controls the mean frequency of SCEs/cell in ≤30 years and ≥30 year's age group was 5.80 and 6.05, respectively. In Smokers SCEs/cell in ≤30 years and ≥30 year's age group was 7.7 and 8.8, respectively. In Smokers with alcohol habit SCEs/cell in ≤30 years and ≥30 years age group was 10.1 and 12.8, respectively.Conclusions:In this study, the duration of the smoking habit has shown a positive correlation with the mean SCE frequency. Whereas, frequency of the habit did not show any influence on the SCE levels. In smokers with alcohol habit, both the duration and frequency of their smoking habit has shown a significant effect on the SCE levels suggesting a synergistic effect of alcohol and smoking leading to excessive DNA damage.
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