Detection of DNA-Psoralen Photoadducts in Mammalian Skin

Pathak, Madhu A.; Zarebska, Z; Mihm, Martín C.; Jarzabek-Chorzelska, M; Chorzelski, Tadeusz P.; Jabłońska, Stefania

doi:10.1111/1523-1747.ep12285506

Cited by 19 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 A,B) but not at 4 mm or 10 mm (data not shown). The dose at 1 mm depth is likely to be the biologicallyrelevant dose because the depth of the dermis (from where fibroblasts are primarily being analyzed) is approximately 0.1 to 1.5 mm from the surface [14,18,24,30,41]. Furthermore, a depth of 1-2 mm has been reported in the literature as relevant for skin irradiation [19,37].…”

Section: Resultsmentioning

confidence: 99%

Studies of the in vivo radiosensitivity of human skin fibroblasts

Hill

Kaspler

Griffin

et al. 2007

Radiotherapy and Oncology

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Studies of the in vivo radiosensitivity of human skin fibroblasts

Hill

Kaspler

Griffin

et al. 2007

Radiotherapy and Oncology

View full text Add to dashboard Cite

show abstract

“…Furthermore, Pathak et al . reported that PUVA produced deoxyribonucleic acid psoralen photoadducts in mammalian skin, indicating nuclear damage of both the keratinocytes and the melanocytes 12 …”

Section: Discussionmentioning

confidence: 99%

Case of leukoderma punctata after topical PUVA treatment

Park

Lee

2004

Int J Dermatology

View full text Add to dashboard Cite

An 11‐year‐old Korean girl presented with a 2‐year history of asymptomatic multiple punctiform, hypopigmented and achromic spots predominantly on the outer aspect of her right forearm. She had been treated for segmental vitiligo on her right forearm with topical PUVA six times a week for 4 years at a local clinic. The lesions were very distinct, round hypopigmented macules 1–2 mm in diameter, predominantly in nonfollicular distribution (Fig. 1). Not only the site where topical psolaren had been applied but also areas on her right forearm where UVA was exposed for a long time for treatment of vitiligo developed multiple punctiform hypopigmented spots. She also had PUVA lentigenes on her right forearm. Laboratory studies including complete blood cell count, biochemistry, and endocrinologic studies were all within normal limits. There was no history of inflammatory lesions antedating the hypopigmentation. Skin biopsy was taken from punctiform hypopigmented macules. There were no specific findings in the hematoxylin‐eosin stained sections. However, marked reduction of melanin and melanocytes was noted with the Fontana‐Masson stain (Fig. 2) and the S‐100 stain (Fig. 3). With these clinical and histological findings the patient was diagnosed as having leukoderma punctata. 1 Multiple, hypopigmented, and achromic spots on the right forearm 2 Microscopic examination of a hypopigmented macule showing marked reduction of melanin (Fontana‐Masson stain, ×200) 3 S‐100 stain showing marked reduction of melanocytes in the basal layer (S‐100 stain, ×200)

show abstract

“…After 20 rain in a dimly lit environment, 35 lal.cm -2 of the 8-MOP or CPZ solution (2.27 • IO -3 M) was applied onto both skin parts. (The amount of 8-MOP applied per cm 2 is in the same order of magnitude as that used by Pathak et al (1985) on human and guinea pig skin. As it appeared that the absorption of radiation in the spectral region of th UV-A lamps for equimolar solutions of CPZ and 8-MOP does not differ significantly, the CPZ concentration was chosen the same).…”

Section: Methodsmentioning

confidence: 99%

Effect of alpha-tocopherol and di-butyl-hydroxytoluene (BHT) on UV-A-induced photobinding of 8-methoxypsoralen to Wistar rat epidermal biomacromolecules in vivo

1991

View full text Add to dashboard Cite

The possible formation of singlet oxygen via photoexcited psoralens has been associated with the occurrence of, amongst others, erythema. Therefore it has been suggested to combine PUVA with the topical or systemic administration of antioxidants. However, the effect of these antioxidants on the photobinding of psoralens to DNA, which is held responsible for the anti-proliferative effect, should be taken into account. In the present study the effect of two phenolic antioxidants, alpha-tocopherol (AT) and butylated hydroxytoluene (BHT), on the in vivo photobinding of 8-methoxypsoralen (8-MOP) to not only epidermal DNA, but also proteins and lipids was determined. After topical application of an ethanolic antioxidant solution onto the shaven skin of Wistar rats, labeled 8-MOP was applied using the same solvent. After this the rats were exposed to UV-A. By separating epidermal lipids, DNA/RNA and proteins by a selective extraction method, irreversible binding of 8-MOP to each of these biomacromolecules was determined. Both AT and BHT caused a decrease in the photobinding of 8-MOP to epidermal DNA and proteins. To investigate the underlying mechanism of this protection, the effect of AT was compared with that of AT-acetate. It also proved helpful to study the effects of the antioxidants on the photobinding of another photosensitizer, namely chlorpromazine. From these experiments it was concluded that AT and BHT affect 8-MOP photobinding by quenching reactive 8-MOP intermediates, involving the phenolic hydroxyl group of the antioxidants. BHT offered protection against lipid binding of 8-MOP but AT, especially at high concentrations, enhanced the UV-A-induced binding of 8-MOP to lipids.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Detection of DNA-Psoralen Photoadducts in Mammalian Skin

Cited by 19 publications

References 21 publications

Studies of the in vivo radiosensitivity of human skin fibroblasts

Studies of the in vivo radiosensitivity of human skin fibroblasts

Case of leukoderma punctata after topical PUVA treatment

Effect of alpha-tocopherol and di-butyl-hydroxytoluene (BHT) on UV-A-induced photobinding of 8-methoxypsoralen to Wistar rat epidermal biomacromolecules in vivo

Contact Info

Product

Resources

About