Studies of the in vivo radiosensitivity of human skin fibroblasts

Hill, Richard P.; Kaspler, Pavel; Griffin, Anthony M.; O’Sullivan, Brian; Catton, Charles; Alasti, Hamideh; Abbas, Ahmar; Heydarian, Moustafa; Ferguson, Peter C.; Wunder, Jay S.; Bell, Robert S.

doi:10.1016/j.radonc.2007.05.025

Cited by 16 publications

(14 citation statements)

References 48 publications

(61 reference statements)

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“…These doses were also chosen to cover a range approximately equivalent to the fractionated treatment received by human skin in our previous study (1). The animals were irradiated at 9 weeks of age, and we assayed the skin 1 day or 2 months after irradiation.…”

Section: Resultsmentioning

confidence: 99%

“…The animals were irradiated at 9 weeks of age, and we assayed the skin 1 day or 2 months after irradiation. These times were chosen based on our previous studies in lung (1 day) and in human skin (1), where it represents the approximate time at which most of the skin specimens were analyzed relative to the end of the course of fractionated radiation treatment. The results of these studies are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In a recent paper (1) we reported that dermal fibroblasts obtained from human skin during surgery for soft tissue sarcoma at 2-9 weeks after the end of a course of fractionated radiotherapy (50 Gy in 25 fractions) demonstrated substantial levels of DNA damage when examined at their first division in culture after removal from the patient. Estimates of the dose received by the skin samples demonstrated that the levels of micronuclei observed were dependent on dose.…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of Micronuclei in Rat Skin Fibroblasts after X Irradiation

2009

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In a previous study, we demonstrated DNA damage, expressed as micronuclei, in binucleate dermal fibroblasts obtained from human skin 2-9 weeks after fractionated radiotherapy. Here we assessed micronuclei in X-irradiated skin fibroblasts from 9-14-week-old female Lewis rats as a function of time after a single dose of radiation to determine the lifetime of such damage in the skin. After irradiation with 5, 10, 15 and 18 Gy, formation of micronuclei at 1 day or 2 months postirradiation increased up to about 10 Gy, with evidence for a plateau at higher doses. The time course of micronuclei present in the skin fibroblasts demonstrated a plateau region (approximately 20 days after 18 Gy and about 2 months after 10 Gy) before the number of micronuclei started to decline. Residual micronuclei were observed for more than 1 year after irradiation. Monomicronucleated cells predominated in fibroblasts from nonirradiated skin, whereas in fibroblasts from irradiated skin, multimicronucleated cells predominated and persisted (together with monomicronucleated cells) in the residual levels of damage at late times. The results suggest that DNA damage in dermal fibroblasts can be assayed by the micronucleus assay in samples from irradiated skin up to 1 month after irradiation for doses up to at least 10 Gy. Further studies are needed to define the dose-response relationship in detail.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dynamics of Micronuclei in Rat Skin Fibroblasts after X Irradiation

2009

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“…Radiosensitivity in different cell types is a limiting factor for use of radiotherapy in many clinical complications including cancers [28][29][30] . Therefore, much interest in normal tissue response to ionizing radiation has emerged.…”

Section: Discussionmentioning

confidence: 99%

Intrinsic radiosensitivity correlated with radiation-induced ROS and cell cycle regulation

Seong

Kim

Jeon

et al. 2010

Mol. Cell. Toxicol.

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“…For example, micronuclei in skin fibroblasts are dependent on radiation dose (34). Cytogenetic analysis using skinpunch biopsies at various locations can define the extent of local radiation exposure.…”

Section: Cutaneous Bioindicators and Medical Managementmentioning

confidence: 99%

Synopsis of Partial-Body Radiation Diagnostic Biomarkers and Medical Management of Radiation Injury Workshop

et al. 2010

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Radiation exposures from accidents, nuclear detonations or terrorist incidents are unlikely to be homogeneous; however, current biodosimetric approaches are developed and validated primarily in whole-body irradiation models. A workshop was held at the Armed Forces Radiobiology Research Institute in May 2008 to draw attention to the need for partial-body biodosimetry, to discuss current knowledge, and to identify the gaps to be filled. A panel of international experts and the workshop attendees discussed the requirements and concepts for a path forward. This report addresses eight key areas identified by the Workshop Program Committee for future focus: (1) improved cytogenetics, (2) clinical signs and symptoms, (3) cutaneous bioindicators, (4) organ-specific biomarkers, (5) biophysical markers of dose, (6) integrated diagnostic approaches, (7) confounding factors, and (8) requirements for post-event medical follow-up. For each area, the status, advantages and limitations of existing approaches and suggestions for new directions are presented.

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Studies of the in vivo radiosensitivity of human skin fibroblasts

Cited by 16 publications

References 48 publications

Dynamics of Micronuclei in Rat Skin Fibroblasts after X Irradiation

Dynamics of Micronuclei in Rat Skin Fibroblasts after X Irradiation

Intrinsic radiosensitivity correlated with radiation-induced ROS and cell cycle regulation

Synopsis of Partial-Body Radiation Diagnostic Biomarkers and Medical Management of Radiation Injury Workshop

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